Regulation of Escherichia coli glnB, prsA, and speA by the purine repressor

B. He, Kang-Yell Choi, H. Zalkin

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

A strategy was devised to identify Escherichia coli genes subject to coregulation by purR. From a data base search, similarities to the pur regulon cis-acting control site were found in 26 E. coli genes. Of five genes examined in which the putative pur operator is upstream of the coding sequence, glnB, prsA, and speA bound purified purine repressor in vitro. Binding of the repressor to a pur operator in these genes was dependent upon a corepressor. The pur operator in glnB is located between two major transcription start sites that were located by primer extension mapping. The effect of purR on expression of glnB, prsA, and speA was examined by using a lacZ reporter. The results indicated two- to threefold repression of these genes by purR. Coregulation by purR provides evidence that expands the pur regulon to include glnB, prsA, and speA. These genes have functions related to nucleotide metabolism.

Original languageEnglish
Pages (from-to)3598-3606
Number of pages9
JournalJournal of Bacteriology
Volume175
Issue number11
DOIs
Publication statusPublished - 1993 Jan 1

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Escherichia coli
Genes
Regulon
Co-Repressor Proteins
Transcription Initiation Site
purine
Nucleotides
Databases

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

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abstract = "A strategy was devised to identify Escherichia coli genes subject to coregulation by purR. From a data base search, similarities to the pur regulon cis-acting control site were found in 26 E. coli genes. Of five genes examined in which the putative pur operator is upstream of the coding sequence, glnB, prsA, and speA bound purified purine repressor in vitro. Binding of the repressor to a pur operator in these genes was dependent upon a corepressor. The pur operator in glnB is located between two major transcription start sites that were located by primer extension mapping. The effect of purR on expression of glnB, prsA, and speA was examined by using a lacZ reporter. The results indicated two- to threefold repression of these genes by purR. Coregulation by purR provides evidence that expands the pur regulon to include glnB, prsA, and speA. These genes have functions related to nucleotide metabolism.",
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Regulation of Escherichia coli glnB, prsA, and speA by the purine repressor. / He, B.; Choi, Kang-Yell; Zalkin, H.

In: Journal of Bacteriology, Vol. 175, No. 11, 01.01.1993, p. 3598-3606.

Research output: Contribution to journalArticle

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