Regulation of hepatitis C virus replication by the core protein through its interaction with viral RNA polymerase

Su Min Kang, Jin Kyu Choi, Seong Jun Kim, Jung Hee Kim, Dae Gyun Ahn, Jong-Won Oh

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The hepatitis C virus (HCV) core protein is a structural component of the nucleocapsid and has been shown to modulate cellular signaling pathways by interaction with various cellular proteins. In the present study, we investigated the role of HCV core protein in viral RNA replication. Immunoprecipitation experiments demonstrated that the core protein binds to the amino-terminal region of RNA-dependent RNA polymerase (RdRp), which encompasses the finger and palm domains. Direct interaction between HCV RdRp and core protein led to inhibition of RdRp RNA synthesis activity of in vitro. Furthermore, over-expression of core protein, but not its derivatives lacking the RdRp-interacting domain, suppressed HCV replication in a hepatoma cell line harboring an HCV subgenomic replicon RNA. Collectively, our results suggest that the core protein, through binding to RdRp and inhibiting its RNA synthesis activity, is a viral regulator of HCV RNA replication.

Original languageEnglish
Pages (from-to)55-59
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume386
Issue number1
DOIs
Publication statusPublished - 2009 Aug 14

Fingerprint

RNA Replicase
Viral RNA
DNA-Directed RNA Polymerases
Virus Replication
Viruses
Hepacivirus
RNA
Proteins
Cell signaling
Nucleocapsid
Replicon
Immunoprecipitation
Protein Binding
Fingers
Hepatocellular Carcinoma
Cells
Hepatitis C virus nucleocapsid protein
Derivatives
Cell Line
Experiments

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Kang, Su Min ; Choi, Jin Kyu ; Kim, Seong Jun ; Kim, Jung Hee ; Ahn, Dae Gyun ; Oh, Jong-Won. / Regulation of hepatitis C virus replication by the core protein through its interaction with viral RNA polymerase. In: Biochemical and Biophysical Research Communications. 2009 ; Vol. 386, No. 1. pp. 55-59.
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Regulation of hepatitis C virus replication by the core protein through its interaction with viral RNA polymerase. / Kang, Su Min; Choi, Jin Kyu; Kim, Seong Jun; Kim, Jung Hee; Ahn, Dae Gyun; Oh, Jong-Won.

In: Biochemical and Biophysical Research Communications, Vol. 386, No. 1, 14.08.2009, p. 55-59.

Research output: Contribution to journalArticle

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