Regulation of inositol phospholipid binding and signaling through syndecan-4

John R. Couchman, Susan Vogt, Ssang Taek Lim, Yangmi Lim, Eok Soo Oh, Glenn D. Prestwich, Anne Theibert, Weon Tae Lee, Anne Woods

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Syndecan-4 is a transmembrane heparan sulfate proteoglycan that can regulate cell-matrix interactions and is enriched in focal adhesions. Its cytoplasmic domain contains a central region unlike that of any other vertebrate or invertebrate syndecan core protein with a cationic motif that binds inositol phospholipids. In turn, lipid binding stabilizes the syndecan in oligomeric form, with subsequent binding and activation of protein kinase C. The specificity of phospholipid binding and its potential regulation are investigated here. Highest affinity of the syndecan-4 cytoplasmic domain was seen with phosphatidylinositol 4,5-bisphosphate (PtdIns-(4,5P)2) and phosphatidylinositol 4-phosphate, and both promoted syndecan-4 oligomerization. Affinity was much reduced for 3-phosphorylated inositides while no binding of diacylglycerol was detected. Syndecan-2 cytoplasmic domain had negligible affinity for any lipid examined. Inositol hexakisphosphate, but not inositol tetrakisphosphate, also had high affinity for the syndecan-4 cytoplasmic domain and could compete effectively with PtdIns(4,5)P2. Since inositol hexaphosphate binding to syndecan-4 does not promote oligomer formation, it is a potential down-regulator of syndecan-4 signaling. Similarly, phosphorylation of serine 183 in syndecan-4 cytoplasmic domain reduced PtdIns(4,5)P2 binding affinity by over 100-fold, although interaction could still be detected by nuclear magnetic resonance spectroscopy. Only protein kinaseCα was up-regulated in activity by the combination of syndecan-4 and PtdIns(4,5)P2, with all other isoforms tested showing minimal response. This is consistent with the codistribution of syndecan-4 with the α isoform of protein kinase C in focal adhesions.

Original languageEnglish
Pages (from-to)49296-49303
Number of pages8
JournalJournal of Biological Chemistry
Volume277
Issue number51
DOIs
Publication statusPublished - 2002 Dec 20

Fingerprint

Syndecan-4
Phosphatidylinositols
Phosphatidylinositol 4,5-Diphosphate
Syndecans
Phytic Acid
Focal Adhesions
Protein Kinase C
Protein Isoforms
Syndecan-2
Adhesion
Lipids
Heparan Sulfate Proteoglycans
Oligomerization
Phosphorylation
Diglycerides
Inositol
Invertebrates
Oligomers
Cell Communication
Serine

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Couchman, J. R., Vogt, S., Lim, S. T., Lim, Y., Oh, E. S., Prestwich, G. D., ... Woods, A. (2002). Regulation of inositol phospholipid binding and signaling through syndecan-4. Journal of Biological Chemistry, 277(51), 49296-49303. https://doi.org/10.1074/jbc.M209679200
Couchman, John R. ; Vogt, Susan ; Lim, Ssang Taek ; Lim, Yangmi ; Oh, Eok Soo ; Prestwich, Glenn D. ; Theibert, Anne ; Lee, Weon Tae ; Woods, Anne. / Regulation of inositol phospholipid binding and signaling through syndecan-4. In: Journal of Biological Chemistry. 2002 ; Vol. 277, No. 51. pp. 49296-49303.
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Couchman, JR, Vogt, S, Lim, ST, Lim, Y, Oh, ES, Prestwich, GD, Theibert, A, Lee, WT & Woods, A 2002, 'Regulation of inositol phospholipid binding and signaling through syndecan-4', Journal of Biological Chemistry, vol. 277, no. 51, pp. 49296-49303. https://doi.org/10.1074/jbc.M209679200

Regulation of inositol phospholipid binding and signaling through syndecan-4. / Couchman, John R.; Vogt, Susan; Lim, Ssang Taek; Lim, Yangmi; Oh, Eok Soo; Prestwich, Glenn D.; Theibert, Anne; Lee, Weon Tae; Woods, Anne.

In: Journal of Biological Chemistry, Vol. 277, No. 51, 20.12.2002, p. 49296-49303.

Research output: Contribution to journalArticle

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Couchman JR, Vogt S, Lim ST, Lim Y, Oh ES, Prestwich GD et al. Regulation of inositol phospholipid binding and signaling through syndecan-4. Journal of Biological Chemistry. 2002 Dec 20;277(51):49296-49303. https://doi.org/10.1074/jbc.M209679200