Regulation of membrane-associated mucins in the human corneal epithelial cells by dexamethasone

Kyoung Yul Seo, So Hyang Chung, Joon H. Lee, Mi Young Park, Eung Kweon Kim

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

PURPOSE: To study the influence of dexamethasone on membrane-associated mucins produced by human corneal epithelial cells. METHODS: Human corneal epithelial cells were cultured in medium supplemented with various concentrations of dexamethasone (ranging from 10 to 10 M). Reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis using monoclonal antibodies specific for human MUC1 (HMFG-1), MUC4 (1G8), and MUC16 (OC125) were performed to evaluate the effect of dexamethasone on membrane-associated mucin expression. The effect of glucocorticoid receptor antagonist (RU38486) on dexamethasone-induced mucin expression was estimated. RESULTS: RT-PCR revealed that MUC1 and MUC16 gene expression were upregulated 48 hours after addition of dexamethasone and that MUC4 gene expression was downregulated in the same condition. Western blot analysis showed that MUC1 and MUC16 proteins were increased after addition of dexamethasone. However, MUC4 was not detected by anti-MUC4 monoclonal antibody (1G8) for ASGP-2 under our conditions. Treatment with RU38486 inhibited the changes of MUC1, MUC4, and MUC16 by dexamethasone; thus, the effect of dexamethasone on mucin expression is mediated by glucocorticoid receptors. CONCLUSIONS: This study shows that MUC1, MUC4, and MUC16 are regulated differently by dexamethasone in human corneal epithelial cells. External application of dexamethasone might affect the precorneal mucin.

Original languageEnglish
Pages (from-to)709-714
Number of pages6
JournalCornea
Volume26
Issue number6
DOIs
Publication statusPublished - 2007 Jul 1

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Mucins
Dexamethasone
Epithelial Cells
Membranes
Glucocorticoid Receptors
Reverse Transcription
Mucin-4
Western Blotting
Monoclonal Antibodies
Gene Expression
Polymerase Chain Reaction
Down-Regulation

All Science Journal Classification (ASJC) codes

  • Ophthalmology

Cite this

Seo, Kyoung Yul ; Chung, So Hyang ; Lee, Joon H. ; Park, Mi Young ; Kim, Eung Kweon. / Regulation of membrane-associated mucins in the human corneal epithelial cells by dexamethasone. In: Cornea. 2007 ; Vol. 26, No. 6. pp. 709-714.
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Regulation of membrane-associated mucins in the human corneal epithelial cells by dexamethasone. / Seo, Kyoung Yul; Chung, So Hyang; Lee, Joon H.; Park, Mi Young; Kim, Eung Kweon.

In: Cornea, Vol. 26, No. 6, 01.07.2007, p. 709-714.

Research output: Contribution to journalArticle

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AU - Seo, Kyoung Yul

AU - Chung, So Hyang

AU - Lee, Joon H.

AU - Park, Mi Young

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N2 - PURPOSE: To study the influence of dexamethasone on membrane-associated mucins produced by human corneal epithelial cells. METHODS: Human corneal epithelial cells were cultured in medium supplemented with various concentrations of dexamethasone (ranging from 10 to 10 M). Reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis using monoclonal antibodies specific for human MUC1 (HMFG-1), MUC4 (1G8), and MUC16 (OC125) were performed to evaluate the effect of dexamethasone on membrane-associated mucin expression. The effect of glucocorticoid receptor antagonist (RU38486) on dexamethasone-induced mucin expression was estimated. RESULTS: RT-PCR revealed that MUC1 and MUC16 gene expression were upregulated 48 hours after addition of dexamethasone and that MUC4 gene expression was downregulated in the same condition. Western blot analysis showed that MUC1 and MUC16 proteins were increased after addition of dexamethasone. However, MUC4 was not detected by anti-MUC4 monoclonal antibody (1G8) for ASGP-2 under our conditions. Treatment with RU38486 inhibited the changes of MUC1, MUC4, and MUC16 by dexamethasone; thus, the effect of dexamethasone on mucin expression is mediated by glucocorticoid receptors. CONCLUSIONS: This study shows that MUC1, MUC4, and MUC16 are regulated differently by dexamethasone in human corneal epithelial cells. External application of dexamethasone might affect the precorneal mucin.

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