Regulation of MIR165/166 by class II and class III homeodomain leucine zipper proteins establishes leaf polarity

Paz Merelo, Hathi Ram, Monica Pia Caggiano, Carolyn Ohno, Felix Ott, Daniel Straub, Moritz Graeff, Seok Keun Cho, Seong Wook Yang, Stephan Wenkel, Marcus G. Heisler

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

A defining feature of plant leaves is their flattened shape. This shape depends on an antagonism between the genes that specify adaxial (top) and abaxial (bottom) tissue identity; however, the molecular nature of this antagonism remains poorly understood. Class III homeodomain leucine zipper (HD-ZIP) transcription factors are key mediators in the regulation of adaxial-abaxial patterning. Their expression is restricted adaxially during early development by the abaxially expressed microRNA (MIR)165/166, yet the mechanism that restricts MIR165/166 expression to abaxial leaf tissues remains unknown. Here, we show that class III and class II HD-ZIP proteins act together to repress MIR165/166 via a conserved cis-element in their promoters. Organ morphology and tissue patterning in plants, therefore, depend on a bidirectional repressive circuit involving a set of miRNAs and its targets.

Original languageEnglish
Pages (from-to)11973-11978
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume113
Issue number42
DOIs
Publication statusPublished - 2016 Oct 18

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Leucine Zippers
MicroRNAs
Proteins
Plant Leaves
Transcription Factors
Genes

All Science Journal Classification (ASJC) codes

  • General

Cite this

Merelo, Paz ; Ram, Hathi ; Caggiano, Monica Pia ; Ohno, Carolyn ; Ott, Felix ; Straub, Daniel ; Graeff, Moritz ; Cho, Seok Keun ; Yang, Seong Wook ; Wenkel, Stephan ; Heisler, Marcus G. / Regulation of MIR165/166 by class II and class III homeodomain leucine zipper proteins establishes leaf polarity. In: Proceedings of the National Academy of Sciences of the United States of America. 2016 ; Vol. 113, No. 42. pp. 11973-11978.
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Regulation of MIR165/166 by class II and class III homeodomain leucine zipper proteins establishes leaf polarity. / Merelo, Paz; Ram, Hathi; Caggiano, Monica Pia; Ohno, Carolyn; Ott, Felix; Straub, Daniel; Graeff, Moritz; Cho, Seok Keun; Yang, Seong Wook; Wenkel, Stephan; Heisler, Marcus G.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, No. 42, 18.10.2016, p. 11973-11978.

Research output: Contribution to journalArticle

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AU - Straub, Daniel

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