Regulation of pluripotency and differentiation by deubiquitinating enzymes

B. Suresh, Jongeun Lee, H. Kim, S. Ramakrishna

Research output: Contribution to journalReview article

11 Citations (Scopus)

Abstract

Post-translational modifications (PTMs) of stemness-related proteins are essential for stem cell maintenance and differentiation. In stem cell self-renewal and differentiation, PTM of stemness-related proteins is tightly regulated because the modified proteins execute various stem cell fate choices. Ubiquitination and deubiquitination, which regulate protein turnover of several stemness-related proteins, must be carefully coordinated to ensure optimal embryonic stem cell maintenance and differentiation. Deubiquitinating enzymes (DUBs), which specifically disassemble ubiquitin chains, are a central component in the ubiquitin-proteasome pathway. These enzymes often control the balance between ubiquitination and deubiquitination. To maintain stemness and achieve efficient differentiation, the ubiquitination and deubiquitination molecular switches must operate in a balanced manner. Here we summarize the current information on DUBs, with a focus on their regulation of stem cell fate determination and deubiquitinase inhibition as a therapeutic strategy. Furthermore, we discuss the possibility of using DUBs with defined stem cell transcription factors to enhance cellular reprogramming efficiency and cell fate conversion. Our review provides new insight into DUB activity by emphasizing their cellular role in regulating stem cell fate. This role paves the way for future research focused on specific DUBs or deubiquitinated substrates as key regulators of pluripotency and stem cell differentiation.

Original languageEnglish
Pages (from-to)1257-1264
Number of pages8
JournalCell Death and Differentiation
Volume23
Issue number8
DOIs
Publication statusPublished - 2016 Aug 1

Fingerprint

Stem Cells
Ubiquitination
Cell Differentiation
Post Translational Protein Processing
Ubiquitin
Proteins
Maintenance
Stem Cell Factor
Proteasome Endopeptidase Complex
Embryonic Stem Cells
Transcription Factors
Deubiquitinating Enzymes
Enzymes
Therapeutics

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Suresh, B. ; Lee, Jongeun ; Kim, H. ; Ramakrishna, S. / Regulation of pluripotency and differentiation by deubiquitinating enzymes. In: Cell Death and Differentiation. 2016 ; Vol. 23, No. 8. pp. 1257-1264.
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Regulation of pluripotency and differentiation by deubiquitinating enzymes. / Suresh, B.; Lee, Jongeun; Kim, H.; Ramakrishna, S.

In: Cell Death and Differentiation, Vol. 23, No. 8, 01.08.2016, p. 1257-1264.

Research output: Contribution to journalReview article

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