Regulation of stomatal development by stomatal lineage miRNAs

Jiali Zhu, Ji Hwan Park, Seulbee Lee, Jae Ho Lee, Daehee Hwang, June M. Kwak, Yun Ju Kim

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Stomata in the plant epidermis play a critical role in growth and survival by controlling gas exchange, transpiration, and immunity to pathogens. Plants modulate stomatal cell fate and patterning through key transcriptional factors and signaling pathways. MicroRNAs (miRNAs) are known to contribute to developmental plasticity in multicellular organisms; however, no miRNAs appear to target the known regulators of stomatal development. It remains unclear as to whether miRNAs are involved in stomatal development. Here, we report highly dynamic, developmentally stage-specific miRNA expression profiles from stomatal lineage cells. We demonstrate that stomatal lineage miRNAs positively and negatively regulate stomatal formation and patterning to avoid clustered stomata. Target prediction of stomatal lineage miRNAs implicates potential cellular processes in stomatal development. We show that miR399-mediated PHO2 regulation, involved in phosphate homeostasis, contributes to the control of stomatal development. Our study demonstrates that miRNAs constitute a critical component in the regulatory mechanisms controlling stomatal development.

Original languageEnglish
Pages (from-to)6237-6245
Number of pages9
JournalProceedings of the National Academy of Sciences of the United States of America
Volume117
Issue number11
DOIs
Publication statusPublished - 2020 Mar 17

Bibliographical note

Funding Information:
ACKNOWLEDGMENTS. We thank Myeong Min Lee for critical reading of the manuscript, Jiyul Jung for the pho2 seeds, Juan Dong for the ML1p::YFP-RCI2A and SPCHp::SPCH-GFP transgenic plants, Dominique C. Bergmann for the MUTEp::nucGFP transgenic plants, Yunde Zhao for the MIR mutant seeds, and Seung-Jin Lee for the technical support. This work was supported by Grant IBS-R013-G2 from the Institute for Basic Science and in part by a grant from National Research Foundation (2019R1A2C3007376) and start-up funds from DGIST to J.M.K., and in part by Grant IBS‐R013‐D1 from the Institute for Basic Science.

Funding Information:
We thank Myeong Min Lee for critical reading of the manuscript, Jiyul Jung for the pho2 seeds, Juan Dong for the ML1p::YFPRCI2A and SPCHp::SPCH-GFP transgenic plants, Dominique C. Bergmann for the MUTEp::nucGFP transgenic plants, Yunde Zhao for the MIR mutant seeds, and Seung-Jin Lee for the technical support. This work was supported by Grant IBS-R013-G2 from the Institute for Basic Science and in part by a grant from National Research Foundation (2019R1A2C3007376) and start-up funds from DGIST to J.M.K., and in part by Grant IBS?R013?D1 from the Institute for Basic Science.

Publisher Copyright:
© 2020 National Academy of Sciences. All rights reserved.

All Science Journal Classification (ASJC) codes

  • General

Fingerprint

Dive into the research topics of 'Regulation of stomatal development by stomatal lineage miRNAs'. Together they form a unique fingerprint.

Cite this