Regulation of the cell expansion gene RHD3 during Arabidopsis development

Haiyang Wang, Myeong Min Lee, John W. Schiefelbein

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The RHD3 (ROOT HAIR DEFECTIVE3) gene encodes a putative GTP-binding protein required for appropriate cell enlargement in Arabidopsis. To obtain insight into the mechanisms of RHD3 regulation, we conducted a molecular genetic dissection of RHD3 gene expression and function. Gene fusion and complementation studies show that the RHD3 gene is highly expressed throughout Arabidopsis development and is controlled by two major regulatory regions. One regulatory region is located between -1,500 and -600 bp upstream of the RHD3 gene and is required for vascular tissue expression. The other region is intragenically located and includes the 558-bp first intron, which is responsible for high-level expression of RHD3 throughout the plant. The presence and location of this intron is essential for gene function because constructs lacking this intron or constructs with the intron in an abnormal position are unable to functionally complement the rhd3 mutations. We also analyzed the role of other RHD genes and the plant hormones auxin and ethylene in RHD3 regulation, and we determined that these act downstream or independently from the RHD3 pathway. This study shows that multiple levels of regulation are employed to ensure the appropriate expression of RHD3 throughout Arabidopsis development.

Original languageEnglish
Pages (from-to)638-649
Number of pages12
JournalPlant Physiology
Volume129
Issue number2
DOIs
Publication statusPublished - 2002 Jan 1

Fingerprint

Arabidopsis
Introns
introns
Nucleic Acid Regulatory Sequences
Genes
genes
Cell Enlargement
cells
Indoleacetic Acids
Plant Growth Regulators
gene fusion
Gene Fusion
Essential Genes
vascular tissues
G-proteins
GTP-Binding Proteins
molecular genetics
plant hormones
Blood Vessels
Dissection

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

Cite this

Wang, Haiyang ; Lee, Myeong Min ; Schiefelbein, John W. / Regulation of the cell expansion gene RHD3 during Arabidopsis development. In: Plant Physiology. 2002 ; Vol. 129, No. 2. pp. 638-649.
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Regulation of the cell expansion gene RHD3 during Arabidopsis development. / Wang, Haiyang; Lee, Myeong Min; Schiefelbein, John W.

In: Plant Physiology, Vol. 129, No. 2, 01.01.2002, p. 638-649.

Research output: Contribution to journalArticle

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