Regulation of thioredoxin peroxidase activity by C-terminal truncation

K. H. Koo, S. Lee, S. Y. Jeong, E. T. Kim, H. J. Kim, K. Kim, Kiwon Song, H. Z. Chae

Research output: Contribution to journalArticle

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Abstract

Thioredoxin peroxidase is a member of peroxiredoxin (Prx) family, which uses a thioredoxin (Trx) as an immediate electron donor for the reduction of peroxide. We have identified C-terminal truncated TPx from Schizosaccharomyces pombe and also have found the truncated form is significantly tenacious against the inactivation of H2O2 than the intact form. Peroxidase assay of a series of recombinant C-terminal truncation mutants (Δ192, Δ191, Δ188, Δ184, Δ176, and Δ165) revealed that TPx could be inactivated (Δ192), reactivated (Δ191-Δ176) and reinactivated (Δ165) by serial truncation from C-terminus. We did not find any significant kinetic difference among reactivated forms; however, distinctive loss of affinity to H2O2 (Km = 5 μM) than that of the intact form (< <5 μM, undeterminable) was monitored. Characterization of a series of Lys191 point mutants manifested that the loss of affinity caused by a deprivation of positive charge born in Lys191 and the loss of affinity resulted in the resistibility to H2O2. Disk inhibition assay with S. pombe cells overexpressing wild-type, Δ192 and Δ191 mutants evidenced that the truncated forms functioning in vitro as well as in vivo.

Original languageEnglish
Pages (from-to)312-318
Number of pages7
JournalArchives of Biochemistry and Biophysics
Volume397
Issue number2
DOIs
Publication statusPublished - 2002 Jan 15

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Peroxiredoxins
Schizosaccharomyces
Thioredoxins
Assays
Peroxides
Peroxidase
Electrons
Kinetics
In Vitro Techniques
Oraconal
Inhibition (Psychology)

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Koo, K. H., Lee, S., Jeong, S. Y., Kim, E. T., Kim, H. J., Kim, K., ... Chae, H. Z. (2002). Regulation of thioredoxin peroxidase activity by C-terminal truncation. Archives of Biochemistry and Biophysics, 397(2), 312-318. https://doi.org/10.1006/abbi.2001.2700
Koo, K. H. ; Lee, S. ; Jeong, S. Y. ; Kim, E. T. ; Kim, H. J. ; Kim, K. ; Song, Kiwon ; Chae, H. Z. / Regulation of thioredoxin peroxidase activity by C-terminal truncation. In: Archives of Biochemistry and Biophysics. 2002 ; Vol. 397, No. 2. pp. 312-318.
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Koo, KH, Lee, S, Jeong, SY, Kim, ET, Kim, HJ, Kim, K, Song, K & Chae, HZ 2002, 'Regulation of thioredoxin peroxidase activity by C-terminal truncation', Archives of Biochemistry and Biophysics, vol. 397, no. 2, pp. 312-318. https://doi.org/10.1006/abbi.2001.2700

Regulation of thioredoxin peroxidase activity by C-terminal truncation. / Koo, K. H.; Lee, S.; Jeong, S. Y.; Kim, E. T.; Kim, H. J.; Kim, K.; Song, Kiwon; Chae, H. Z.

In: Archives of Biochemistry and Biophysics, Vol. 397, No. 2, 15.01.2002, p. 312-318.

Research output: Contribution to journalArticle

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