Regulatory effect of humoral milieu on the viral DNA and surface antigen expression of hepatitis B virus (HBV) in vitro

Hong Jai Lee; Bo Young Shin; Jae Seung Moon; Chun Chang Ho; Jin Su Shin; Jee Sang Yoon; Jee Eun Yang; Soo Ki Kim; Sang Kyou Lee

doi:10.1007/s13273-019-0015-y

Regulatory effect of humoral milieu on the viral DNA and surface antigen expression of hepatitis B virus (HBV) in vitro

Hong Jai Lee, Bo Young Shin, Jae Seung Moon, Chun Chang Ho, Jin Su Shin, Jee Sang Yoon, Jee Eun Yang, Soo Ki Kim, Sang Kyou Lee

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Backgrounds: While a few virions of hepatitis B virus (HBV) are sufficient to liver infection in vivo, even a large quantity of virion is unable to enter into hepatoma cells in vitro. Methods: To solve this paradox, we explored if humoral milieu such as serum or culture media, and its constituents, and pH would regulate the viral DNA and surface antigen expression of HBV in vitro. Results: The viral DNA and surface antigen expression of HBV are significantly more stable in human serum than in mouse serum, and Dulbecco’s Modified eagle Medium (DMEM). In line, the increase of anti-HBcAg antibody in mouse serum, and DMEM implies that the virion was cleared in non-host humoral milieu. Further, lipid analysis revealed higher levels of lipids in human serum than those in mouse serum, and DMEM. Lipid removal analysis showed the decreased level of HBV DNA and surface antigen expression in human and mouse serum. In electrolytes analysis, human serum contained lower level of potassium than mouse serum. Last, on the viral DNA and surface antigen expression of HBV, the alkalinity of DMEM was more effective than neutral pH. Conclusion: Collectively, this study indicates that humoral milieu would regulate the viral DNA and surface antigen expression of HBV in vitro. This is the first report to regulate HBV growth/viability via simple manipulation of host humoral milieu.

Original language	English
Pages (from-to)	123-128
Number of pages	6
Journal	Molecular and Cellular Toxicology
Volume	15
Issue number	2
DOIs	https://doi.org/10.1007/s13273-019-0015-y
Publication status	Published - 2019 Apr 1

Bibliographical note

Funding Information:
Acknowledgements This work was partially supported by the research grant from Good T Cells; the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. NRF-2017R1A2A1A17 069807; NRF-2018R1D1A1B07048194); Global Research Laboratory (GRL) Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF-2016K1A1A2912755).

Publisher Copyright:
© 2019, The Korean Society of Toxicogenomics and Toxicoproteomics and Springer Nature B.V.

All Science Journal Classification (ASJC) codes

Pathology and Forensic Medicine
Toxicology
Pharmacology, Toxicology and Pharmaceutics(all)
Public Health, Environmental and Occupational Health
Health, Toxicology and Mutagenesis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s13273-019-0015-y

Cite this

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title = "Regulatory effect of humoral milieu on the viral DNA and surface antigen expression of hepatitis B virus (HBV) in vitro",

abstract = "Backgrounds: While a few virions of hepatitis B virus (HBV) are sufficient to liver infection in vivo, even a large quantity of virion is unable to enter into hepatoma cells in vitro. Methods: To solve this paradox, we explored if humoral milieu such as serum or culture media, and its constituents, and pH would regulate the viral DNA and surface antigen expression of HBV in vitro. Results: The viral DNA and surface antigen expression of HBV are significantly more stable in human serum than in mouse serum, and Dulbecco{\textquoteright}s Modified eagle Medium (DMEM). In line, the increase of anti-HBcAg antibody in mouse serum, and DMEM implies that the virion was cleared in non-host humoral milieu. Further, lipid analysis revealed higher levels of lipids in human serum than those in mouse serum, and DMEM. Lipid removal analysis showed the decreased level of HBV DNA and surface antigen expression in human and mouse serum. In electrolytes analysis, human serum contained lower level of potassium than mouse serum. Last, on the viral DNA and surface antigen expression of HBV, the alkalinity of DMEM was more effective than neutral pH. Conclusion: Collectively, this study indicates that humoral milieu would regulate the viral DNA and surface antigen expression of HBV in vitro. This is the first report to regulate HBV growth/viability via simple manipulation of host humoral milieu.",

author = "Lee, {Hong Jai} and Shin, {Bo Young} and Moon, {Jae Seung} and Ho, {Chun Chang} and Shin, {Jin Su} and Yoon, {Jee Sang} and Yang, {Jee Eun} and Kim, {Soo Ki} and Lee, {Sang Kyou}",

note = "Funding Information: Acknowledgements This work was partially supported by the research grant from Good T Cells; the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. NRF-2017R1A2A1A17 069807; NRF-2018R1D1A1B07048194); Global Research Laboratory (GRL) Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF-2016K1A1A2912755). Publisher Copyright: {\textcopyright} 2019, The Korean Society of Toxicogenomics and Toxicoproteomics and Springer Nature B.V.",

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doi = "10.1007/s13273-019-0015-y",

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AU - Lee, Hong Jai

AU - Shin, Bo Young

AU - Moon, Jae Seung

AU - Ho, Chun Chang

AU - Shin, Jin Su

AU - Yoon, Jee Sang

AU - Yang, Jee Eun

AU - Kim, Soo Ki

AU - Lee, Sang Kyou

N1 - Funding Information: Acknowledgements This work was partially supported by the research grant from Good T Cells; the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. NRF-2017R1A2A1A17 069807; NRF-2018R1D1A1B07048194); Global Research Laboratory (GRL) Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF-2016K1A1A2912755). Publisher Copyright: © 2019, The Korean Society of Toxicogenomics and Toxicoproteomics and Springer Nature B.V.

PY - 2019/4/1

Y1 - 2019/4/1

N2 - Backgrounds: While a few virions of hepatitis B virus (HBV) are sufficient to liver infection in vivo, even a large quantity of virion is unable to enter into hepatoma cells in vitro. Methods: To solve this paradox, we explored if humoral milieu such as serum or culture media, and its constituents, and pH would regulate the viral DNA and surface antigen expression of HBV in vitro. Results: The viral DNA and surface antigen expression of HBV are significantly more stable in human serum than in mouse serum, and Dulbecco’s Modified eagle Medium (DMEM). In line, the increase of anti-HBcAg antibody in mouse serum, and DMEM implies that the virion was cleared in non-host humoral milieu. Further, lipid analysis revealed higher levels of lipids in human serum than those in mouse serum, and DMEM. Lipid removal analysis showed the decreased level of HBV DNA and surface antigen expression in human and mouse serum. In electrolytes analysis, human serum contained lower level of potassium than mouse serum. Last, on the viral DNA and surface antigen expression of HBV, the alkalinity of DMEM was more effective than neutral pH. Conclusion: Collectively, this study indicates that humoral milieu would regulate the viral DNA and surface antigen expression of HBV in vitro. This is the first report to regulate HBV growth/viability via simple manipulation of host humoral milieu.

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Regulatory effect of humoral milieu on the viral DNA and surface antigen expression of hepatitis B virus (HBV) in vitro

Abstract

Bibliographical note

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UN SDGs

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