Relationship between Epstein-Barr virus-encoded RNA expression, apoptosis and lymphocytic infiltration in gastric carcinoma with lymphoid-rich stroma

Mee Yon Cho, Tae Heon Kim, Sang Yeop Yi, Woo Hee Jung, Kwang Hwa Park

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Abstract

Objective: To investigate the association of apoptosis with apoptotic proteins and Epstein-Barr virus (EBV) expression in gastric carcinoma with lymphoid-rich stroma (GCLRS) in order to clarify the relationship between lymphocytic infi ltration, EBV expression and apoptosis in gastric carcinoma. Subjects and Methods: We performed immunohistochemical stains for bcl-2, bax and in situ hybridization for EBV-encoded RNA and the terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end labeling technique using the 24 cases of GCLRS and 23 cases of gastric carcinoma with lymphoid-poor stroma (GCLPS) out of 1,635 cases of gastric carcinoma. Results: The prevalence of GCLRS was 1.47% (24/1,635). The prevalence of EBV involvement in GCLRS (79%, 19/24) was significantly higher than in GCLPS (8.7%, 2/23). Apoptosis was revealed in all cases and there was no statistical difference between GCLRS and GCLPS. Immunohistochemical expression for bcl-2 was revealed only in the cytoplasm of lymphocytes in all cases, but bax showed distinct expression in the cytoplasm of neoplastic cells in all cases. There was a significant correlation between bax expression and the apoptotic index (p < 0.05). Lymphocytic infiltration was significantly correlated with the expression of EBV (p < 0.05) but not with survival rate and apoptosis. Most of the GCLRS cases were rated as low clinical stage (stage IA or B) and seemed to have a good prognosis. Conclusions: These results support the hypothesis that EBV infection plays an important role in the tumorigenesis of GCLRS, which has a better prognosis than usual gastric adenocarcinoma although there was no significant difference in the apoptotic index between GCLRS and GCLPS.

Original languageEnglish
Pages (from-to)353-360
Number of pages8
JournalMedical Principles and Practice
Volume13
Issue number6
DOIs
Publication statusPublished - 2004 Oct 27

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Human Herpesvirus 4
Stomach
RNA
Apoptosis
Carcinoma
Cytoplasm
Digoxigenin
Epstein-Barr Virus Infections
DNA Nucleotidylexotransferase
In Situ Hybridization
Carcinogenesis
Adenocarcinoma
Coloring Agents
Survival Rate

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

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title = "Relationship between Epstein-Barr virus-encoded RNA expression, apoptosis and lymphocytic infiltration in gastric carcinoma with lymphoid-rich stroma",
abstract = "Objective: To investigate the association of apoptosis with apoptotic proteins and Epstein-Barr virus (EBV) expression in gastric carcinoma with lymphoid-rich stroma (GCLRS) in order to clarify the relationship between lymphocytic infi ltration, EBV expression and apoptosis in gastric carcinoma. Subjects and Methods: We performed immunohistochemical stains for bcl-2, bax and in situ hybridization for EBV-encoded RNA and the terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end labeling technique using the 24 cases of GCLRS and 23 cases of gastric carcinoma with lymphoid-poor stroma (GCLPS) out of 1,635 cases of gastric carcinoma. Results: The prevalence of GCLRS was 1.47{\%} (24/1,635). The prevalence of EBV involvement in GCLRS (79{\%}, 19/24) was significantly higher than in GCLPS (8.7{\%}, 2/23). Apoptosis was revealed in all cases and there was no statistical difference between GCLRS and GCLPS. Immunohistochemical expression for bcl-2 was revealed only in the cytoplasm of lymphocytes in all cases, but bax showed distinct expression in the cytoplasm of neoplastic cells in all cases. There was a significant correlation between bax expression and the apoptotic index (p < 0.05). Lymphocytic infiltration was significantly correlated with the expression of EBV (p < 0.05) but not with survival rate and apoptosis. Most of the GCLRS cases were rated as low clinical stage (stage IA or B) and seemed to have a good prognosis. Conclusions: These results support the hypothesis that EBV infection plays an important role in the tumorigenesis of GCLRS, which has a better prognosis than usual gastric adenocarcinoma although there was no significant difference in the apoptotic index between GCLRS and GCLPS.",
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Relationship between Epstein-Barr virus-encoded RNA expression, apoptosis and lymphocytic infiltration in gastric carcinoma with lymphoid-rich stroma. / Cho, Mee Yon; Kim, Tae Heon; Yi, Sang Yeop; Jung, Woo Hee; Park, Kwang Hwa.

In: Medical Principles and Practice, Vol. 13, No. 6, 27.10.2004, p. 353-360.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Relationship between Epstein-Barr virus-encoded RNA expression, apoptosis and lymphocytic infiltration in gastric carcinoma with lymphoid-rich stroma

AU - Cho, Mee Yon

AU - Kim, Tae Heon

AU - Yi, Sang Yeop

AU - Jung, Woo Hee

AU - Park, Kwang Hwa

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N2 - Objective: To investigate the association of apoptosis with apoptotic proteins and Epstein-Barr virus (EBV) expression in gastric carcinoma with lymphoid-rich stroma (GCLRS) in order to clarify the relationship between lymphocytic infi ltration, EBV expression and apoptosis in gastric carcinoma. Subjects and Methods: We performed immunohistochemical stains for bcl-2, bax and in situ hybridization for EBV-encoded RNA and the terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end labeling technique using the 24 cases of GCLRS and 23 cases of gastric carcinoma with lymphoid-poor stroma (GCLPS) out of 1,635 cases of gastric carcinoma. Results: The prevalence of GCLRS was 1.47% (24/1,635). The prevalence of EBV involvement in GCLRS (79%, 19/24) was significantly higher than in GCLPS (8.7%, 2/23). Apoptosis was revealed in all cases and there was no statistical difference between GCLRS and GCLPS. Immunohistochemical expression for bcl-2 was revealed only in the cytoplasm of lymphocytes in all cases, but bax showed distinct expression in the cytoplasm of neoplastic cells in all cases. There was a significant correlation between bax expression and the apoptotic index (p < 0.05). Lymphocytic infiltration was significantly correlated with the expression of EBV (p < 0.05) but not with survival rate and apoptosis. Most of the GCLRS cases were rated as low clinical stage (stage IA or B) and seemed to have a good prognosis. Conclusions: These results support the hypothesis that EBV infection plays an important role in the tumorigenesis of GCLRS, which has a better prognosis than usual gastric adenocarcinoma although there was no significant difference in the apoptotic index between GCLRS and GCLPS.

AB - Objective: To investigate the association of apoptosis with apoptotic proteins and Epstein-Barr virus (EBV) expression in gastric carcinoma with lymphoid-rich stroma (GCLRS) in order to clarify the relationship between lymphocytic infi ltration, EBV expression and apoptosis in gastric carcinoma. Subjects and Methods: We performed immunohistochemical stains for bcl-2, bax and in situ hybridization for EBV-encoded RNA and the terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end labeling technique using the 24 cases of GCLRS and 23 cases of gastric carcinoma with lymphoid-poor stroma (GCLPS) out of 1,635 cases of gastric carcinoma. Results: The prevalence of GCLRS was 1.47% (24/1,635). The prevalence of EBV involvement in GCLRS (79%, 19/24) was significantly higher than in GCLPS (8.7%, 2/23). Apoptosis was revealed in all cases and there was no statistical difference between GCLRS and GCLPS. Immunohistochemical expression for bcl-2 was revealed only in the cytoplasm of lymphocytes in all cases, but bax showed distinct expression in the cytoplasm of neoplastic cells in all cases. There was a significant correlation between bax expression and the apoptotic index (p < 0.05). Lymphocytic infiltration was significantly correlated with the expression of EBV (p < 0.05) but not with survival rate and apoptosis. Most of the GCLRS cases were rated as low clinical stage (stage IA or B) and seemed to have a good prognosis. Conclusions: These results support the hypothesis that EBV infection plays an important role in the tumorigenesis of GCLRS, which has a better prognosis than usual gastric adenocarcinoma although there was no significant difference in the apoptotic index between GCLRS and GCLPS.

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