Relationship between gonadotropin-releasing hormone/gonadotropin-releasing hormone receptor signaling and progesterone receptors in human trophoblasts

Bang Hyun Lee, Jae-Hoon Kim, Tae Chul Park, Hee Joong Lee

Research output: Contribution to journalArticle

Abstract

OBJECTIVE: To investigate the relationship between gonadotropin-releasing hormone (GnRH)/gonadotropinreleasing hormone receptor (GnRHR) signaling and progesterone receptors (PGRs). STUDY DESIGN: HTR-8/SV neo cells and JEG-3 cells were incubated for 24 hours with GnRH-I or -II. The expression of PGRs was measured by quantitative realtime reverse transcriptase-polymerase chain reaction and Western blotting. RESULTS: In both cells lines after incubation with 1-1,000 nM/L GnRH-I or -II the ratio of PGR-B to PGR-AB mRNA level did not differ in any of the groups as compared to the control. Relatively higher expression of PGR-B than PGR-A mRNA and expression of only PGR-B protein without PGR-A protein were observed in all specimens. There were no significant differences in the expression of PGR-B protein in each group when compared to the control group after incubation with GnRH-I or -II at 0.001-1,000 mM/L in both cell lines. CONCLUSION: PGRs are expressed regardless of GnRH/GnRHR signaling in human trophoblasts, in which PGR-B expression is more dominant than PGR-A expression.

Original languageEnglish
Pages (from-to)275-281
Number of pages7
JournalJournal of Reproductive Medicine
Volume61
Issue number3
Publication statusPublished - 2016 Jun 1

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LHRH Receptors
Trophoblasts
Progesterone Receptors
Gonadotropin-Releasing Hormone
Hormones
Cell Line
Messenger RNA
Proteins
Reverse Transcriptase Polymerase Chain Reaction
Western Blotting
progesterone receptor B
Control Groups
progesterone receptor A

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Obstetrics and Gynaecology

Cite this

@article{f4bd629755b1433d80875e56d6e5eafc,
title = "Relationship between gonadotropin-releasing hormone/gonadotropin-releasing hormone receptor signaling and progesterone receptors in human trophoblasts",
abstract = "OBJECTIVE: To investigate the relationship between gonadotropin-releasing hormone (GnRH)/gonadotropinreleasing hormone receptor (GnRHR) signaling and progesterone receptors (PGRs). STUDY DESIGN: HTR-8/SV neo cells and JEG-3 cells were incubated for 24 hours with GnRH-I or -II. The expression of PGRs was measured by quantitative realtime reverse transcriptase-polymerase chain reaction and Western blotting. RESULTS: In both cells lines after incubation with 1-1,000 nM/L GnRH-I or -II the ratio of PGR-B to PGR-AB mRNA level did not differ in any of the groups as compared to the control. Relatively higher expression of PGR-B than PGR-A mRNA and expression of only PGR-B protein without PGR-A protein were observed in all specimens. There were no significant differences in the expression of PGR-B protein in each group when compared to the control group after incubation with GnRH-I or -II at 0.001-1,000 mM/L in both cell lines. CONCLUSION: PGRs are expressed regardless of GnRH/GnRHR signaling in human trophoblasts, in which PGR-B expression is more dominant than PGR-A expression.",
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Relationship between gonadotropin-releasing hormone/gonadotropin-releasing hormone receptor signaling and progesterone receptors in human trophoblasts. / Lee, Bang Hyun; Kim, Jae-Hoon; Park, Tae Chul; Lee, Hee Joong.

In: Journal of Reproductive Medicine, Vol. 61, No. 3, 01.06.2016, p. 275-281.

Research output: Contribution to journalArticle

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T1 - Relationship between gonadotropin-releasing hormone/gonadotropin-releasing hormone receptor signaling and progesterone receptors in human trophoblasts

AU - Lee, Bang Hyun

AU - Kim, Jae-Hoon

AU - Park, Tae Chul

AU - Lee, Hee Joong

PY - 2016/6/1

Y1 - 2016/6/1

N2 - OBJECTIVE: To investigate the relationship between gonadotropin-releasing hormone (GnRH)/gonadotropinreleasing hormone receptor (GnRHR) signaling and progesterone receptors (PGRs). STUDY DESIGN: HTR-8/SV neo cells and JEG-3 cells were incubated for 24 hours with GnRH-I or -II. The expression of PGRs was measured by quantitative realtime reverse transcriptase-polymerase chain reaction and Western blotting. RESULTS: In both cells lines after incubation with 1-1,000 nM/L GnRH-I or -II the ratio of PGR-B to PGR-AB mRNA level did not differ in any of the groups as compared to the control. Relatively higher expression of PGR-B than PGR-A mRNA and expression of only PGR-B protein without PGR-A protein were observed in all specimens. There were no significant differences in the expression of PGR-B protein in each group when compared to the control group after incubation with GnRH-I or -II at 0.001-1,000 mM/L in both cell lines. CONCLUSION: PGRs are expressed regardless of GnRH/GnRHR signaling in human trophoblasts, in which PGR-B expression is more dominant than PGR-A expression.

AB - OBJECTIVE: To investigate the relationship between gonadotropin-releasing hormone (GnRH)/gonadotropinreleasing hormone receptor (GnRHR) signaling and progesterone receptors (PGRs). STUDY DESIGN: HTR-8/SV neo cells and JEG-3 cells were incubated for 24 hours with GnRH-I or -II. The expression of PGRs was measured by quantitative realtime reverse transcriptase-polymerase chain reaction and Western blotting. RESULTS: In both cells lines after incubation with 1-1,000 nM/L GnRH-I or -II the ratio of PGR-B to PGR-AB mRNA level did not differ in any of the groups as compared to the control. Relatively higher expression of PGR-B than PGR-A mRNA and expression of only PGR-B protein without PGR-A protein were observed in all specimens. There were no significant differences in the expression of PGR-B protein in each group when compared to the control group after incubation with GnRH-I or -II at 0.001-1,000 mM/L in both cell lines. CONCLUSION: PGRs are expressed regardless of GnRH/GnRHR signaling in human trophoblasts, in which PGR-B expression is more dominant than PGR-A expression.

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