Relaxin modulates the expression of MMPs and TIMPs in fibroblasts of patients with carpal tunnel syndrome

Young Mi Kang, Hwan Mo Lee, Seong Hwan Moon, Ho Kang, Yun Rak Choi

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Purpose: The aim of this study was to investigate the anti-fibrotic effect of relaxin in subsynovial fibroblasts activated by transforming growth factor beta (TGF-β). Materials and Methods: To test the anti-fibrotic effect of an adenovirus-relaxin construct (Ad-RLN) on subsynovial fibroblasts in vitro, cells from subsynovial connective tissue of patients with carpal tunnel syndrome were activated with TGF-β1 and exposed to Ad-RLN (as a therapeutic gene) or adenovirus-lacZ construct (as a marker gene) for four hours. Subsynovial fibroblast cultures without adenoviral exposure served as controls. Results: We observed induction of gene expressions of collagen I, III and IV, as well as the abatement of alpha-smooth muscle actin (a-SMA) synthesis, Smad2 phosphorylation, and fibronectin at the protein level, in comparison to controls. In addition, protein expressions of matrix metalloproteinase (MMP) I was significantly induced, whereas the protein expressions of tissue inhibitor of metalloproteinases (TIMP) I and IV were reduced due to relaxin expression. Conclusion: RLN prevents excessive synthesis of extracellular matrix by reducing the expressions of its components, such as fibronectin, a-SMA, and phosphorylated Smad2, by increasing the expression of MMPs; and by decreasing the expression of TIMPs.

Original languageEnglish
Pages (from-to)415-422
Number of pages8
JournalYonsei medical journal
Volume58
Issue number2
DOIs
Publication statusPublished - 2017 Mar

Bibliographical note

Funding Information:
We would like to thank Chae-Ok Yun, Ph.D. (Department of Bioengineering, College of Engineering, Hanyang University, Seoul, Korea) for providing the adenovirus-relaxin construct. This work was supported, in part, by the Brain Korea 21 PLUS Project for Medical Science at Yonsei University. This study was supported by a faculty research program from Yonsei University College of Medicine (6-2014-0098), as well as the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Science, ICT & Future Planning (grant number: 2011-0014399).

Publisher Copyright:
© Yonsei University College of Medicine 2017.

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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