Release of heat shock protein 70 (Hsp70) and the effects of extracellular Hsp70 on matric metalloproteinase-9 expression in human monocytic U937 cells

Kyoung Jin Lee, Yoo Mih Kim, Dae Young Kim, Dooil Jeoung, Kyuhyung Han, Seung-Taek Lee, Yun Sil Lee, Kyeong Han Park, Jeong Hyun Park, Dae Joong Kim, Jang Hee Hahn

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Abstract

Heat shock protein 70 (Hsp70) release and its effects on pro-inflammatory cytokine production have been controversial. In this study, we investigated whether Hsp70 could be released from monocytes and activates matrix metalloproteinase-9 (MMP-9) gene expression. Hsp70 overexpression in human monocytic cell line U937 was found to increase PMA-induced MMP-9 expression and enhance cell motility. Hsp70 cDNA transfectants released Hsp70 protein into culture supernatants, and a part of released Hsp70 subsequently was bound to the surface of U937 cells. Addition of culture medium containing the extracelluar Hsp70 led to an increase not only in proMMP-9 secretion, but also the invasiveness of U937 cells through Matrigel or human umbilical vascular endothelial cells (HUVEC) in vitro. Immunodepletion of Hsp70 abolished its effect on MMP-9 expression. The released Hsp70 activated nuclear factor kappa B (NF-κB) and activating protein-1 (AP-1), which led to the activation of MMP-9 transcription. Taken together, these results suggest that extracellular Hsp70 induces the expression of MMP-9 gene through activation of NF-κB and AP-1.

Original languageEnglish
Pages (from-to)364-374
Number of pages11
JournalJournal of the Chinese Medical Association
Volume38
Issue number4
Publication statusPublished - 2006 Aug 31

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U937 Cells
HSP70 Heat-Shock Proteins
Metalloproteases
Matrix Metalloproteinase 9
NF-kappa B
Umbilicus
Proteins
Transcriptional Activation
Cell Movement
Culture Media
Monocytes
Endothelial Cells
Complementary DNA
Cytokines
Gene Expression
Cell Line

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Lee, Kyoung Jin ; Kim, Yoo Mih ; Kim, Dae Young ; Jeoung, Dooil ; Han, Kyuhyung ; Lee, Seung-Taek ; Lee, Yun Sil ; Park, Kyeong Han ; Park, Jeong Hyun ; Kim, Dae Joong ; Hahn, Jang Hee. / Release of heat shock protein 70 (Hsp70) and the effects of extracellular Hsp70 on matric metalloproteinase-9 expression in human monocytic U937 cells. In: Journal of the Chinese Medical Association. 2006 ; Vol. 38, No. 4. pp. 364-374.
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Release of heat shock protein 70 (Hsp70) and the effects of extracellular Hsp70 on matric metalloproteinase-9 expression in human monocytic U937 cells. / Lee, Kyoung Jin; Kim, Yoo Mih; Kim, Dae Young; Jeoung, Dooil; Han, Kyuhyung; Lee, Seung-Taek; Lee, Yun Sil; Park, Kyeong Han; Park, Jeong Hyun; Kim, Dae Joong; Hahn, Jang Hee.

In: Journal of the Chinese Medical Association, Vol. 38, No. 4, 31.08.2006, p. 364-374.

Research output: Contribution to journalArticle

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