Release of heat shock protein 70 (Hsp70) and the effects of extracellular Hsp70 on matric metalloproteinase-9 expression in human monocytic U937 cells

Kyoung Jin Lee, Mih Kim Yoo, Young Kim Dae, Dooil Jeoung, Kyuhyung Han, Seung-Taek Lee, Yun Sil Lee, Han Park Kyeong, Hyun Park Jeong, Joong Kim Dae, Jang Hee Hahn

Research output: Contribution to journalArticle

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Abstract

Heat shock protein 70 (Hsp70) release and its effects on pro-inflammatory cytokine production have been controversial. In this study, we investigated whether Hsp70 could be released from monocytes and activates matrix metalloproteinase-9 (MMP-9) gene expression. Hsp70 overexpression in human monocytic cell line U937 was found to increase PMA-induced MMP-9 expression and enhance cell motility. Hsp70 cDNA transfectants released Hsp70 protein into culture supernatants, and a part of released Hsp70 subsequently was bound to the surface of U937 cells. Addition of culture medium containing the extracelluar Hsp70 led to an increase not only in proMMP-9 secretion, but also the invasiveness of U937 cells through Matrigel or human umbilical vascular endothelial cells (HUVEC) in vitro. Immunodepletion of Hsp70 abolished its effect on MMP-9 expression. The released Hsp70 activated nuclear factor kappa B (NF-κB) and activating protein-1 (AP-1), which led to the activation of MMP-9 transcription. Taken together, these results suggest that extracellular Hsp70 induces the expression of MMP-9 gene through activation of NF-κB and AP-1.

Original languageEnglish
Pages (from-to)364-374
Number of pages11
JournalExperimental and Molecular Medicine
Volume38
Issue number4
DOIs
Publication statusPublished - 2006 Aug 31

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U937 Cells
HSP70 Heat-Shock Proteins
Metalloproteases
Matrix Metalloproteinase 9
NF-kappa B
Chemical activation
Umbilicus
Proteins
Endothelial cells
Transcription
Gene expression
Transcriptional Activation
Cell Movement
Culture Media
Monocytes
Endothelial Cells
Complementary DNA
Genes
Cells
Cytokines

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

Cite this

Lee, Kyoung Jin ; Yoo, Mih Kim ; Dae, Young Kim ; Jeoung, Dooil ; Han, Kyuhyung ; Lee, Seung-Taek ; Lee, Yun Sil ; Kyeong, Han Park ; Jeong, Hyun Park ; Dae, Joong Kim ; Hahn, Jang Hee. / Release of heat shock protein 70 (Hsp70) and the effects of extracellular Hsp70 on matric metalloproteinase-9 expression in human monocytic U937 cells. In: Experimental and Molecular Medicine. 2006 ; Vol. 38, No. 4. pp. 364-374.
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Release of heat shock protein 70 (Hsp70) and the effects of extracellular Hsp70 on matric metalloproteinase-9 expression in human monocytic U937 cells. / Lee, Kyoung Jin; Yoo, Mih Kim; Dae, Young Kim; Jeoung, Dooil; Han, Kyuhyung; Lee, Seung-Taek; Lee, Yun Sil; Kyeong, Han Park; Jeong, Hyun Park; Dae, Joong Kim; Hahn, Jang Hee.

In: Experimental and Molecular Medicine, Vol. 38, No. 4, 31.08.2006, p. 364-374.

Research output: Contribution to journalArticle

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