Remifentanil protects myocardium through activation of anti-apoptotic pathways of survival in ischemia-reperfused rat heart

H. S. Kim, J. E. Cho, S. W. Hong, S. O. Kim, J. K. Shim, Younglan Kwak

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Abstract

Remifentanil is a commonly used opioid in anesthesia with cardioprotective effect in ischemia-reperfused (I/R) heart. We evaluated the influence of remifentanil on myocardial infarct size and expressions of proteins involved in apoptosis in I/R rat heart following various time protocols of remifentanil administration. Artificially ventilated anesthetized Sprague-Dawley rats were subjected to a 30 min of left anterior descending coronary artery occlusion followed by 2 h of reperfusion. Rats were randomly assigned to one of five groups; Sham, I/R only, remifentanil preconditioning, postconditioning and continuous infusion group. Myocardial infarct size, the phosphorylation of ERK1/2, Bcl2, Bax and cytochrome c and the expression of genes influencing Ca 2+ homeostasis were assessed. In remifentanil-administered rat hearts, regardless of the timing and duration of administration, infarct size was consistently reduced compared to I/R only rats. Remifentanil improved expression of ERK 1/2 and anti-apoptotic protein Bcl2, and expression of sarcoplasmic reticulum genes which were significantly reduced in the I/R rats only. Remifentanil reduced expression of pro-apoptotic protein, Bax and cytochrome c. These suggested that remifentanil produced cardioprotective effect by preserving the expression of proteins involved in anti-apoptotic pathways, and the expression of sarcoplasmic reticulum genes in I/R rat heart, regardless of the timing of administration.

Original languageEnglish
Pages (from-to)347-356
Number of pages10
JournalPhysiological Research
Volume59
Issue number3
Publication statusPublished - 2010 Sep 7

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Myocardium
Ischemia
Apoptosis Regulatory Proteins
Sarcoplasmic Reticulum
Cytochromes c
Myocardial Infarction
remifentanil
Coronary Occlusion
Opioid Analgesics
Genes
Reperfusion
Sprague Dawley Rats
Coronary Vessels
Proteins
Homeostasis
Anesthesia
Phosphorylation
Apoptosis
Gene Expression

All Science Journal Classification (ASJC) codes

  • Physiology

Cite this

Kim, H. S. ; Cho, J. E. ; Hong, S. W. ; Kim, S. O. ; Shim, J. K. ; Kwak, Younglan. / Remifentanil protects myocardium through activation of anti-apoptotic pathways of survival in ischemia-reperfused rat heart. In: Physiological Research. 2010 ; Vol. 59, No. 3. pp. 347-356.
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Remifentanil protects myocardium through activation of anti-apoptotic pathways of survival in ischemia-reperfused rat heart. / Kim, H. S.; Cho, J. E.; Hong, S. W.; Kim, S. O.; Shim, J. K.; Kwak, Younglan.

In: Physiological Research, Vol. 59, No. 3, 07.09.2010, p. 347-356.

Research output: Contribution to journalArticle

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