Abstract
Nitric oxide (NO) is recognized as a mediator and regulator of inflammatory responses. Rengyolone, a cyclohexylethanoid isolated from the fruits of Forsythia koreana, exhibits anti-inflammatory activity with unknown mechanism. In this study, we found that rengyolone has a strong inhibitory effect on the production of nitric oxide (NO) and tumor necrosis factor-α (TNF-α). Rengyolone also inhibited inducible nitric oxide synthase (iNOS) gene expression and cyclooxygenase 2 (COX-2) by lipopolysaccharide (LPS). In order to explore the mechanism responsible for the inhibition of iNOS gene expression by rengyolone, we investigated its effect on LPS-induced nuclear factor-κB (NF-κB) activation. The LPS-induced DNA binding activity of NF-κB was significantly inhibited by rengyolone, and this effect was mediated through inhibition of the degradation of inhibitory factor-κBα and phosphorylation of p38 MAP kinase. Furthermore, rengyolone suppressed the expression of ICE protein in IL-1β-treated D10S cells. Taken together, these results suggest that rengyolone attenuates the inflammation through inhibition of NO production and iNOS expression by blockade of NF-κB and p38 MAPK activation in LPS-stimulated RAW 264.7 cells.
| Original language | English |
|---|---|
| Pages (from-to) | 1198-1205 |
| Number of pages | 8 |
| Journal | Biochemical Pharmacology |
| Volume | 71 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 2006 Apr 14 |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Pharmacology
Cite this
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Rengyolone inhibits inducible nitric oxide synthase expression and nitric oxide production by down-regulation of NF-κB and p38 MAP kinase activity in LPS-stimulated RAW 264.7 cells. / Kim, Jin Hee; Kim, Dong Hyun; Baek, Seung Hwa; Lee, Ho Jae; Kim, Mee Ree; Kwon, Ho Jeong; Lee, Choong Hwan.
In: Biochemical Pharmacology, Vol. 71, No. 8, 14.04.2006, p. 1198-1205.Research output: Contribution to journal › Article
TY - JOUR
T1 - Rengyolone inhibits inducible nitric oxide synthase expression and nitric oxide production by down-regulation of NF-κB and p38 MAP kinase activity in LPS-stimulated RAW 264.7 cells
AU - Kim, Jin Hee
AU - Kim, Dong Hyun
AU - Baek, Seung Hwa
AU - Lee, Ho Jae
AU - Kim, Mee Ree
AU - Kwon, Ho Jeong
AU - Lee, Choong Hwan
PY - 2006/4/14
Y1 - 2006/4/14
N2 - Nitric oxide (NO) is recognized as a mediator and regulator of inflammatory responses. Rengyolone, a cyclohexylethanoid isolated from the fruits of Forsythia koreana, exhibits anti-inflammatory activity with unknown mechanism. In this study, we found that rengyolone has a strong inhibitory effect on the production of nitric oxide (NO) and tumor necrosis factor-α (TNF-α). Rengyolone also inhibited inducible nitric oxide synthase (iNOS) gene expression and cyclooxygenase 2 (COX-2) by lipopolysaccharide (LPS). In order to explore the mechanism responsible for the inhibition of iNOS gene expression by rengyolone, we investigated its effect on LPS-induced nuclear factor-κB (NF-κB) activation. The LPS-induced DNA binding activity of NF-κB was significantly inhibited by rengyolone, and this effect was mediated through inhibition of the degradation of inhibitory factor-κBα and phosphorylation of p38 MAP kinase. Furthermore, rengyolone suppressed the expression of ICE protein in IL-1β-treated D10S cells. Taken together, these results suggest that rengyolone attenuates the inflammation through inhibition of NO production and iNOS expression by blockade of NF-κB and p38 MAPK activation in LPS-stimulated RAW 264.7 cells.
AB - Nitric oxide (NO) is recognized as a mediator and regulator of inflammatory responses. Rengyolone, a cyclohexylethanoid isolated from the fruits of Forsythia koreana, exhibits anti-inflammatory activity with unknown mechanism. In this study, we found that rengyolone has a strong inhibitory effect on the production of nitric oxide (NO) and tumor necrosis factor-α (TNF-α). Rengyolone also inhibited inducible nitric oxide synthase (iNOS) gene expression and cyclooxygenase 2 (COX-2) by lipopolysaccharide (LPS). In order to explore the mechanism responsible for the inhibition of iNOS gene expression by rengyolone, we investigated its effect on LPS-induced nuclear factor-κB (NF-κB) activation. The LPS-induced DNA binding activity of NF-κB was significantly inhibited by rengyolone, and this effect was mediated through inhibition of the degradation of inhibitory factor-κBα and phosphorylation of p38 MAP kinase. Furthermore, rengyolone suppressed the expression of ICE protein in IL-1β-treated D10S cells. Taken together, these results suggest that rengyolone attenuates the inflammation through inhibition of NO production and iNOS expression by blockade of NF-κB and p38 MAPK activation in LPS-stimulated RAW 264.7 cells.
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U2 - 10.1016/j.bcp.2005.12.031
DO - 10.1016/j.bcp.2005.12.031
M3 - Article
VL - 71
SP - 1198
EP - 1205
JO - Biochemical Pharmacology
JF - Biochemical Pharmacology
SN - 0006-2952
IS - 8
ER -