Rengyolone inhibits inducible nitric oxide synthase expression and nitric oxide production by down-regulation of NF-κB and p38 MAP kinase activity in LPS-stimulated RAW 264.7 cells

Jin Hee Kim, Dong Hyun Kim, Seung Hwa Baek, Ho Jae Lee, Mee Ree Kim, Ho Jeong Kwon, Choong Hwan Lee

Research output: Contribution to journalArticlepeer-review

64 Citations (Scopus)

Abstract

Nitric oxide (NO) is recognized as a mediator and regulator of inflammatory responses. Rengyolone, a cyclohexylethanoid isolated from the fruits of Forsythia koreana, exhibits anti-inflammatory activity with unknown mechanism. In this study, we found that rengyolone has a strong inhibitory effect on the production of nitric oxide (NO) and tumor necrosis factor-α (TNF-α). Rengyolone also inhibited inducible nitric oxide synthase (iNOS) gene expression and cyclooxygenase 2 (COX-2) by lipopolysaccharide (LPS). In order to explore the mechanism responsible for the inhibition of iNOS gene expression by rengyolone, we investigated its effect on LPS-induced nuclear factor-κB (NF-κB) activation. The LPS-induced DNA binding activity of NF-κB was significantly inhibited by rengyolone, and this effect was mediated through inhibition of the degradation of inhibitory factor-κBα and phosphorylation of p38 MAP kinase. Furthermore, rengyolone suppressed the expression of ICE protein in IL-1β-treated D10S cells. Taken together, these results suggest that rengyolone attenuates the inflammation through inhibition of NO production and iNOS expression by blockade of NF-κB and p38 MAPK activation in LPS-stimulated RAW 264.7 cells.

Original languageEnglish
Pages (from-to)1198-1205
Number of pages8
JournalBiochemical Pharmacology
Volume71
Issue number8
DOIs
Publication statusPublished - 2006 Apr 14

Bibliographical note

Funding Information:
This research was supported by a grant from the Plant Diversity Research Center of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology (MOST) of the Korean government.

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Pharmacology

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