Renoprotective mechanism of remote ischemic preconditioning based on transcriptomic analysis in a porcine renal ischemia reperfusion injury model

Young Eun Yoon, Kyung Hwa Choi, Sook Young Kim, Young In Cho, Kwang Suk Lee, Kwang Hyun Kim, Seung Choul Yang, Woong Kyu Han

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Ischemic preconditioning (IPC) is a well-known phenomenon in which tissues are exposed to a brief period of ischemia prior to a longer ischemic event. This technique produces tissue tolerance to ischemia reperfusion injury (IRI). Currently, IPC's mechanism of action is poorly understood. Using a porcine single kidney model, we performed remote IPC with renal IRI and evaluated the IPC mechanism of action. Following left nephrectomy, 15 female Yorkshire pigs were divided into three groups: no IPC and 90 minutes of warm ischemia (control), remote IPC immediately followed by 90 minutes of warm ischemia (rIPCe), and remote IPC with 90 minutes of warm ischemia performed 24 hours later (rIPCl). Differential gene expression analysis was performed using a porcine-specific microarray. The microarray analysis of porcine renal tissues identified 1,053 differentially expressed probes in preconditioned pigs. Among these, 179 genes had altered expression in both the rIPCe and rIPCl groups. The genes were largely related to oxidation reduction, apoptosis, and inflammatory response. In the rIPCl group, an additional 848 genes had altered expression levels. These genes were primarily related to immune response and inflammation, including those coding for cytokines and cytokine receptors and those that play roles in the complement system and coagulation cascade. In the complement system, the membrane attack complex was determined to be sublytic, because it colocalized with phosphorylated extracellular signalregulated kinase. Furthermore, alpha 2 macroglobulin, tissue plasminogen activator, uterine plasmin trypsin inhibitor, and arginase-1 mRNA levels were elevated in the rIPCl group. These findings indicate that remote IPC produces renoprotective effects through multiple mechanisms, and these effects develop over a long timeframe rather than immediately following IPC.

Original languageEnglish
Article numbere0141099
JournalPloS one
Volume10
Issue number10
DOIs
Publication statusPublished - 2015 Oct 21

Fingerprint

Ischemic Preconditioning
ischemia
Reperfusion Injury
transcriptomics
Swine
Genes
kidneys
Kidney
swine
Tissue
Microarrays
Warm Ischemia
Complement Membrane Attack Complex
alpha-Macroglobulins
Arginase
Cytokine Receptors
Trypsin Inhibitors
Plasminogen Activators
Fibrinolysin
mechanism of action

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Yoon, Young Eun ; Choi, Kyung Hwa ; Kim, Sook Young ; Cho, Young In ; Lee, Kwang Suk ; Kim, Kwang Hyun ; Yang, Seung Choul ; Han, Woong Kyu. / Renoprotective mechanism of remote ischemic preconditioning based on transcriptomic analysis in a porcine renal ischemia reperfusion injury model. In: PloS one. 2015 ; Vol. 10, No. 10.
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Renoprotective mechanism of remote ischemic preconditioning based on transcriptomic analysis in a porcine renal ischemia reperfusion injury model. / Yoon, Young Eun; Choi, Kyung Hwa; Kim, Sook Young; Cho, Young In; Lee, Kwang Suk; Kim, Kwang Hyun; Yang, Seung Choul; Han, Woong Kyu.

In: PloS one, Vol. 10, No. 10, e0141099, 21.10.2015.

Research output: Contribution to journalArticle

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