Reperfusion cellular injury in an animal model of transient ischemia

Seung Koo Lee, Dong Ik Kim, Si Yeon Kim, Dong Joon Kim, Jongeun Lee, Jae Hwan Kim

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

BACKGROUND AND PURPOSE: Early thrombolytic therapy is encouraged in hyperacute stroke, although this might result in delayed reperfusion injury. Our purpose was to investigate the serial changes in cerebral perfusion following transient ischemia by means of MR imaging and to correlate them with the histologic findings obtained by using the TdT-mediated dUTP-biotin nick-end labeling (TUNEL) assay. METHODS: One-hour transient occlusion of the middle cerebral artery was produced in 10 cats. Serial perfusion-weighted MR imaging was performed for 3 days after reperfusion. The reperfusion characteristics in each region of the brain were classified into four groups according to the serial perfusion MR imaging status: normal perfusion (N), continuous hyperperfusion (I), early hyperperfusion and gradual decrease (II), and persistent hypoperfusion (III). After the last imaging session, a specimen was obtained, and TUNEL staining was performed. TUNEL-positive cells were counted under a high-power-field (HPF) light microscope (×200). The degree of TUNEL positivity was compared among the four reperfusion groups classified on the basis of serial perfusion-weighted imaging findings. RESULTS: Group N had 16.8 ± 5.1 TUNEL-positive cells per HPF. Groups I and II had a statistically significant increase in TUNEL positivity (39.5 ± 13.4 and 43.6 ± 16.7 cells per HPF; P < .01, one-way analysis of variance), whereas group III had a statistically insignificant increase in TUNEL positivity (23.3 ± 6.9 cells per HPF). CONCLUSION: Reperfusion followed by hyperperfusion induced cellular damage, although the initial MR imaging findings were normal. The inclusion of anti-reperfusion injury therapy should be considered in thrombolytic treatment.

Original languageEnglish
Pages (from-to)1342-1347
Number of pages6
JournalAmerican Journal of Neuroradiology
Volume25
Issue number8
Publication statusPublished - 2004 Sep 1

Fingerprint

Biotin
Reperfusion Injury
Ischemia
Animal Models
Reperfusion
Perfusion Imaging
Perfusion
Middle Cerebral Artery Infarction
Thrombolytic Therapy
Secondary Prevention
Analysis of Variance
Cats
Stroke
Staining and Labeling
Light
Brain

All Science Journal Classification (ASJC) codes

  • Radiology Nuclear Medicine and imaging
  • Clinical Neurology

Cite this

Lee, S. K., Kim, D. I., Kim, S. Y., Kim, D. J., Lee, J., & Kim, J. H. (2004). Reperfusion cellular injury in an animal model of transient ischemia. American Journal of Neuroradiology, 25(8), 1342-1347.
Lee, Seung Koo ; Kim, Dong Ik ; Kim, Si Yeon ; Kim, Dong Joon ; Lee, Jongeun ; Kim, Jae Hwan. / Reperfusion cellular injury in an animal model of transient ischemia. In: American Journal of Neuroradiology. 2004 ; Vol. 25, No. 8. pp. 1342-1347.
@article{d4ce1cc40abf40fea5efec1a78ac6c16,
title = "Reperfusion cellular injury in an animal model of transient ischemia",
abstract = "BACKGROUND AND PURPOSE: Early thrombolytic therapy is encouraged in hyperacute stroke, although this might result in delayed reperfusion injury. Our purpose was to investigate the serial changes in cerebral perfusion following transient ischemia by means of MR imaging and to correlate them with the histologic findings obtained by using the TdT-mediated dUTP-biotin nick-end labeling (TUNEL) assay. METHODS: One-hour transient occlusion of the middle cerebral artery was produced in 10 cats. Serial perfusion-weighted MR imaging was performed for 3 days after reperfusion. The reperfusion characteristics in each region of the brain were classified into four groups according to the serial perfusion MR imaging status: normal perfusion (N), continuous hyperperfusion (I), early hyperperfusion and gradual decrease (II), and persistent hypoperfusion (III). After the last imaging session, a specimen was obtained, and TUNEL staining was performed. TUNEL-positive cells were counted under a high-power-field (HPF) light microscope (×200). The degree of TUNEL positivity was compared among the four reperfusion groups classified on the basis of serial perfusion-weighted imaging findings. RESULTS: Group N had 16.8 ± 5.1 TUNEL-positive cells per HPF. Groups I and II had a statistically significant increase in TUNEL positivity (39.5 ± 13.4 and 43.6 ± 16.7 cells per HPF; P < .01, one-way analysis of variance), whereas group III had a statistically insignificant increase in TUNEL positivity (23.3 ± 6.9 cells per HPF). CONCLUSION: Reperfusion followed by hyperperfusion induced cellular damage, although the initial MR imaging findings were normal. The inclusion of anti-reperfusion injury therapy should be considered in thrombolytic treatment.",
author = "Lee, {Seung Koo} and Kim, {Dong Ik} and Kim, {Si Yeon} and Kim, {Dong Joon} and Jongeun Lee and Kim, {Jae Hwan}",
year = "2004",
month = "9",
day = "1",
language = "English",
volume = "25",
pages = "1342--1347",
journal = "American Journal of Neuroradiology",
issn = "0195-6108",
publisher = "American Society of Neuroradiology",
number = "8",

}

Lee, SK, Kim, DI, Kim, SY, Kim, DJ, Lee, J & Kim, JH 2004, 'Reperfusion cellular injury in an animal model of transient ischemia', American Journal of Neuroradiology, vol. 25, no. 8, pp. 1342-1347.

Reperfusion cellular injury in an animal model of transient ischemia. / Lee, Seung Koo; Kim, Dong Ik; Kim, Si Yeon; Kim, Dong Joon; Lee, Jongeun; Kim, Jae Hwan.

In: American Journal of Neuroradiology, Vol. 25, No. 8, 01.09.2004, p. 1342-1347.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Reperfusion cellular injury in an animal model of transient ischemia

AU - Lee, Seung Koo

AU - Kim, Dong Ik

AU - Kim, Si Yeon

AU - Kim, Dong Joon

AU - Lee, Jongeun

AU - Kim, Jae Hwan

PY - 2004/9/1

Y1 - 2004/9/1

N2 - BACKGROUND AND PURPOSE: Early thrombolytic therapy is encouraged in hyperacute stroke, although this might result in delayed reperfusion injury. Our purpose was to investigate the serial changes in cerebral perfusion following transient ischemia by means of MR imaging and to correlate them with the histologic findings obtained by using the TdT-mediated dUTP-biotin nick-end labeling (TUNEL) assay. METHODS: One-hour transient occlusion of the middle cerebral artery was produced in 10 cats. Serial perfusion-weighted MR imaging was performed for 3 days after reperfusion. The reperfusion characteristics in each region of the brain were classified into four groups according to the serial perfusion MR imaging status: normal perfusion (N), continuous hyperperfusion (I), early hyperperfusion and gradual decrease (II), and persistent hypoperfusion (III). After the last imaging session, a specimen was obtained, and TUNEL staining was performed. TUNEL-positive cells were counted under a high-power-field (HPF) light microscope (×200). The degree of TUNEL positivity was compared among the four reperfusion groups classified on the basis of serial perfusion-weighted imaging findings. RESULTS: Group N had 16.8 ± 5.1 TUNEL-positive cells per HPF. Groups I and II had a statistically significant increase in TUNEL positivity (39.5 ± 13.4 and 43.6 ± 16.7 cells per HPF; P < .01, one-way analysis of variance), whereas group III had a statistically insignificant increase in TUNEL positivity (23.3 ± 6.9 cells per HPF). CONCLUSION: Reperfusion followed by hyperperfusion induced cellular damage, although the initial MR imaging findings were normal. The inclusion of anti-reperfusion injury therapy should be considered in thrombolytic treatment.

AB - BACKGROUND AND PURPOSE: Early thrombolytic therapy is encouraged in hyperacute stroke, although this might result in delayed reperfusion injury. Our purpose was to investigate the serial changes in cerebral perfusion following transient ischemia by means of MR imaging and to correlate them with the histologic findings obtained by using the TdT-mediated dUTP-biotin nick-end labeling (TUNEL) assay. METHODS: One-hour transient occlusion of the middle cerebral artery was produced in 10 cats. Serial perfusion-weighted MR imaging was performed for 3 days after reperfusion. The reperfusion characteristics in each region of the brain were classified into four groups according to the serial perfusion MR imaging status: normal perfusion (N), continuous hyperperfusion (I), early hyperperfusion and gradual decrease (II), and persistent hypoperfusion (III). After the last imaging session, a specimen was obtained, and TUNEL staining was performed. TUNEL-positive cells were counted under a high-power-field (HPF) light microscope (×200). The degree of TUNEL positivity was compared among the four reperfusion groups classified on the basis of serial perfusion-weighted imaging findings. RESULTS: Group N had 16.8 ± 5.1 TUNEL-positive cells per HPF. Groups I and II had a statistically significant increase in TUNEL positivity (39.5 ± 13.4 and 43.6 ± 16.7 cells per HPF; P < .01, one-way analysis of variance), whereas group III had a statistically insignificant increase in TUNEL positivity (23.3 ± 6.9 cells per HPF). CONCLUSION: Reperfusion followed by hyperperfusion induced cellular damage, although the initial MR imaging findings were normal. The inclusion of anti-reperfusion injury therapy should be considered in thrombolytic treatment.

UR - http://www.scopus.com/inward/record.url?scp=16544371180&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=16544371180&partnerID=8YFLogxK

M3 - Article

VL - 25

SP - 1342

EP - 1347

JO - American Journal of Neuroradiology

JF - American Journal of Neuroradiology

SN - 0195-6108

IS - 8

ER -