Replacing carbohydrate with protein and fat in prediabetes or type-2 diabetes

Greater effect on metabolites in PBMC than plasma

Minjoo Kim, Gayoung Song, Miso Kang, Hye Jin Yoo, Tae Sook Jeong, Sang Hyun Lee, Jong Ho Lee

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: Active metabolism of peripheral blood mononuclear cells (PBMC) could suggest their suitability for metabolomics studies. This study examined whether reductions in PBMCs and plasma lipoprotein-associated phospholipase A2 (Lp-PLA2) activities induced by dietary intervention affected the overall metabolic profiles of PBMC and plasma. Methods: Eighty nonobese subjects aged 40-70 years (18.5 & BMI < 30 kg/mbsupesup) with prediabetes or newly-diagnosed type-2 diabetes were assigned to consume either the usual refined-rice diet (control group, n = 40) or to replace refined rice with whole grains and legumes as carbohydrates (whole-grain group, n = 40) for three meals per day during the 12-week intervention. Fasting PBMC and plasma metabolomes were profiled using UPLC-LTQ-Orbitrap mass spectrometry. Results: After 12 weeks, changes in fasting glucose, HbAbsubesub, HOMA-IR, MDA, ox-LDL, LDL particle size, plasma Lp-PLA bsubesub activity, and PBMC enzyme activity in the whole-grain group were significantly different from those in the control group before and after adjusting for baseline levels. The PBMC levels of L-leucine, oleamide, lysoPC (16:0), and lysoPC (18:0) in the whole-grain group showed greater reductions compared with those of the control group. Changes in plasma metabolites were not significantly different between the two groups. Changes in PBMC Lp-PLAbsubesub activity positively correlated with changes in L-leucine, oleamide, lysoPC (16:0), lysoPC (18:0), glucose, and ox-LDL, and negatively correlated with changes in LDL particle size. Conclusions: This study showed that dietary intervention in prediabetic or type-2 diabetic patients had a greater effect on PBMC Lp-PLAbsubesub activity and metabolites compared with those of plasma metabolites.

Original languageEnglish
Article number3
JournalNutrition and Metabolism
Volume13
Issue number1
DOIs
Publication statusPublished - 2016 Jan 19

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Prediabetic State
Type 2 Diabetes Mellitus
Blood Cells
Fats
Carbohydrates
Proteins
Metabolome
Particle Size
Leucine
Control Groups
Fasting
1-Alkyl-2-acetylglycerophosphocholine Esterase
Glucose
Metabolomics
Fabaceae
Meals
Mass Spectrometry
Diet
Whole Grains
Enzymes

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Kim, Minjoo ; Song, Gayoung ; Kang, Miso ; Yoo, Hye Jin ; Jeong, Tae Sook ; Lee, Sang Hyun ; Lee, Jong Ho. / Replacing carbohydrate with protein and fat in prediabetes or type-2 diabetes : Greater effect on metabolites in PBMC than plasma. In: Nutrition and Metabolism. 2016 ; Vol. 13, No. 1.
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abstract = "Background: Active metabolism of peripheral blood mononuclear cells (PBMC) could suggest their suitability for metabolomics studies. This study examined whether reductions in PBMCs and plasma lipoprotein-associated phospholipase A2 (Lp-PLA2) activities induced by dietary intervention affected the overall metabolic profiles of PBMC and plasma. Methods: Eighty nonobese subjects aged 40-70 years (18.5 & BMI < 30 kg/mbsupesup) with prediabetes or newly-diagnosed type-2 diabetes were assigned to consume either the usual refined-rice diet (control group, n = 40) or to replace refined rice with whole grains and legumes as carbohydrates (whole-grain group, n = 40) for three meals per day during the 12-week intervention. Fasting PBMC and plasma metabolomes were profiled using UPLC-LTQ-Orbitrap mass spectrometry. Results: After 12 weeks, changes in fasting glucose, HbAbsubesub, HOMA-IR, MDA, ox-LDL, LDL particle size, plasma Lp-PLA bsubesub activity, and PBMC enzyme activity in the whole-grain group were significantly different from those in the control group before and after adjusting for baseline levels. The PBMC levels of L-leucine, oleamide, lysoPC (16:0), and lysoPC (18:0) in the whole-grain group showed greater reductions compared with those of the control group. Changes in plasma metabolites were not significantly different between the two groups. Changes in PBMC Lp-PLAbsubesub activity positively correlated with changes in L-leucine, oleamide, lysoPC (16:0), lysoPC (18:0), glucose, and ox-LDL, and negatively correlated with changes in LDL particle size. Conclusions: This study showed that dietary intervention in prediabetic or type-2 diabetic patients had a greater effect on PBMC Lp-PLAbsubesub activity and metabolites compared with those of plasma metabolites.",
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Replacing carbohydrate with protein and fat in prediabetes or type-2 diabetes : Greater effect on metabolites in PBMC than plasma. / Kim, Minjoo; Song, Gayoung; Kang, Miso; Yoo, Hye Jin; Jeong, Tae Sook; Lee, Sang Hyun; Lee, Jong Ho.

In: Nutrition and Metabolism, Vol. 13, No. 1, 3, 19.01.2016.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Replacing carbohydrate with protein and fat in prediabetes or type-2 diabetes

T2 - Greater effect on metabolites in PBMC than plasma

AU - Kim, Minjoo

AU - Song, Gayoung

AU - Kang, Miso

AU - Yoo, Hye Jin

AU - Jeong, Tae Sook

AU - Lee, Sang Hyun

AU - Lee, Jong Ho

PY - 2016/1/19

Y1 - 2016/1/19

N2 - Background: Active metabolism of peripheral blood mononuclear cells (PBMC) could suggest their suitability for metabolomics studies. This study examined whether reductions in PBMCs and plasma lipoprotein-associated phospholipase A2 (Lp-PLA2) activities induced by dietary intervention affected the overall metabolic profiles of PBMC and plasma. Methods: Eighty nonobese subjects aged 40-70 years (18.5 & BMI < 30 kg/mbsupesup) with prediabetes or newly-diagnosed type-2 diabetes were assigned to consume either the usual refined-rice diet (control group, n = 40) or to replace refined rice with whole grains and legumes as carbohydrates (whole-grain group, n = 40) for three meals per day during the 12-week intervention. Fasting PBMC and plasma metabolomes were profiled using UPLC-LTQ-Orbitrap mass spectrometry. Results: After 12 weeks, changes in fasting glucose, HbAbsubesub, HOMA-IR, MDA, ox-LDL, LDL particle size, plasma Lp-PLA bsubesub activity, and PBMC enzyme activity in the whole-grain group were significantly different from those in the control group before and after adjusting for baseline levels. The PBMC levels of L-leucine, oleamide, lysoPC (16:0), and lysoPC (18:0) in the whole-grain group showed greater reductions compared with those of the control group. Changes in plasma metabolites were not significantly different between the two groups. Changes in PBMC Lp-PLAbsubesub activity positively correlated with changes in L-leucine, oleamide, lysoPC (16:0), lysoPC (18:0), glucose, and ox-LDL, and negatively correlated with changes in LDL particle size. Conclusions: This study showed that dietary intervention in prediabetic or type-2 diabetic patients had a greater effect on PBMC Lp-PLAbsubesub activity and metabolites compared with those of plasma metabolites.

AB - Background: Active metabolism of peripheral blood mononuclear cells (PBMC) could suggest their suitability for metabolomics studies. This study examined whether reductions in PBMCs and plasma lipoprotein-associated phospholipase A2 (Lp-PLA2) activities induced by dietary intervention affected the overall metabolic profiles of PBMC and plasma. Methods: Eighty nonobese subjects aged 40-70 years (18.5 & BMI < 30 kg/mbsupesup) with prediabetes or newly-diagnosed type-2 diabetes were assigned to consume either the usual refined-rice diet (control group, n = 40) or to replace refined rice with whole grains and legumes as carbohydrates (whole-grain group, n = 40) for three meals per day during the 12-week intervention. Fasting PBMC and plasma metabolomes were profiled using UPLC-LTQ-Orbitrap mass spectrometry. Results: After 12 weeks, changes in fasting glucose, HbAbsubesub, HOMA-IR, MDA, ox-LDL, LDL particle size, plasma Lp-PLA bsubesub activity, and PBMC enzyme activity in the whole-grain group were significantly different from those in the control group before and after adjusting for baseline levels. The PBMC levels of L-leucine, oleamide, lysoPC (16:0), and lysoPC (18:0) in the whole-grain group showed greater reductions compared with those of the control group. Changes in plasma metabolites were not significantly different between the two groups. Changes in PBMC Lp-PLAbsubesub activity positively correlated with changes in L-leucine, oleamide, lysoPC (16:0), lysoPC (18:0), glucose, and ox-LDL, and negatively correlated with changes in LDL particle size. Conclusions: This study showed that dietary intervention in prediabetic or type-2 diabetic patients had a greater effect on PBMC Lp-PLAbsubesub activity and metabolites compared with those of plasma metabolites.

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