Requirement for Pak3 in Rac1-induced organization of actin and myosin during Drosophila larval wound healing

Seung Hee Baek, Hae Weon Cho, Young Chang Kwon, Ji Hyun Lee, Moon Jong Kim, Hyangkyu Lee, Kwang-Min Choe

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Rho-family small GTPases regulate epithelial cell sheet migration by organizing actin and myosin during wound healing. Here, we report that Pak3, but not Pak1, is a downstream target protein for Rac1 in wound closure of the Drosophila larval epidermis. Pak3-deficient larvae failed to close a wound hole and this defect was not rescued by Pak1 expression, indicating differential functions of the two proteins. Pak3 localized to the wound margin, which selectively required Rac1. Pak3-deficient larvae showed severe defects in actin-myosin organization at the wound margin and in submarginal cells, which was reminiscent of the phenotypes of Rac1-deficient larvae. These results suggest that Pak3 specifically mediates Rac1 signaling in organizing actin and myosin during Drosophila epidermal wound healing.

Original languageEnglish
Pages (from-to)772-777
Number of pages6
JournalFEBS Letters
Volume586
Issue number6
DOIs
Publication statusPublished - 2012 Mar 23

Fingerprint

Myosins
Wound Healing
Drosophila
Actins
Larva
Wounds and Injuries
rac1 GTP-Binding Protein
Defects
Monomeric GTP-Binding Proteins
Epidermis
Cell Movement
Epithelial Cells
Phenotype
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Baek, Seung Hee ; Cho, Hae Weon ; Kwon, Young Chang ; Lee, Ji Hyun ; Kim, Moon Jong ; Lee, Hyangkyu ; Choe, Kwang-Min. / Requirement for Pak3 in Rac1-induced organization of actin and myosin during Drosophila larval wound healing. In: FEBS Letters. 2012 ; Vol. 586, No. 6. pp. 772-777.
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abstract = "Rho-family small GTPases regulate epithelial cell sheet migration by organizing actin and myosin during wound healing. Here, we report that Pak3, but not Pak1, is a downstream target protein for Rac1 in wound closure of the Drosophila larval epidermis. Pak3-deficient larvae failed to close a wound hole and this defect was not rescued by Pak1 expression, indicating differential functions of the two proteins. Pak3 localized to the wound margin, which selectively required Rac1. Pak3-deficient larvae showed severe defects in actin-myosin organization at the wound margin and in submarginal cells, which was reminiscent of the phenotypes of Rac1-deficient larvae. These results suggest that Pak3 specifically mediates Rac1 signaling in organizing actin and myosin during Drosophila epidermal wound healing.",
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Requirement for Pak3 in Rac1-induced organization of actin and myosin during Drosophila larval wound healing. / Baek, Seung Hee; Cho, Hae Weon; Kwon, Young Chang; Lee, Ji Hyun; Kim, Moon Jong; Lee, Hyangkyu; Choe, Kwang-Min.

In: FEBS Letters, Vol. 586, No. 6, 23.03.2012, p. 772-777.

Research output: Contribution to journalArticle

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AU - Baek, Seung Hee

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AB - Rho-family small GTPases regulate epithelial cell sheet migration by organizing actin and myosin during wound healing. Here, we report that Pak3, but not Pak1, is a downstream target protein for Rac1 in wound closure of the Drosophila larval epidermis. Pak3-deficient larvae failed to close a wound hole and this defect was not rescued by Pak1 expression, indicating differential functions of the two proteins. Pak3 localized to the wound margin, which selectively required Rac1. Pak3-deficient larvae showed severe defects in actin-myosin organization at the wound margin and in submarginal cells, which was reminiscent of the phenotypes of Rac1-deficient larvae. These results suggest that Pak3 specifically mediates Rac1 signaling in organizing actin and myosin during Drosophila epidermal wound healing.

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