Requirement for Pak3 in Rac1-induced organization of actin and myosin during Drosophila larval wound healing

Seung Hee Baek, Hae Weon Cho, Young Chang Kwon, Ji Hyun Lee, Moon Jong Kim, Hyangkyu Lee, Kwang Min Choe

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Rho-family small GTPases regulate epithelial cell sheet migration by organizing actin and myosin during wound healing. Here, we report that Pak3, but not Pak1, is a downstream target protein for Rac1 in wound closure of the Drosophila larval epidermis. Pak3-deficient larvae failed to close a wound hole and this defect was not rescued by Pak1 expression, indicating differential functions of the two proteins. Pak3 localized to the wound margin, which selectively required Rac1. Pak3-deficient larvae showed severe defects in actin-myosin organization at the wound margin and in submarginal cells, which was reminiscent of the phenotypes of Rac1-deficient larvae. These results suggest that Pak3 specifically mediates Rac1 signaling in organizing actin and myosin during Drosophila epidermal wound healing.

Original languageEnglish
Pages (from-to)772-777
Number of pages6
JournalFEBS Letters
Volume586
Issue number6
DOIs
Publication statusPublished - 2012 Mar 23

Fingerprint

Myosins
Wound Healing
Drosophila
Actins
Larva
Wounds and Injuries
rac1 GTP-Binding Protein
Defects
Monomeric GTP-Binding Proteins
Epidermis
Cell Movement
Epithelial Cells
Phenotype
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Baek, Seung Hee ; Cho, Hae Weon ; Kwon, Young Chang ; Lee, Ji Hyun ; Kim, Moon Jong ; Lee, Hyangkyu ; Choe, Kwang Min. / Requirement for Pak3 in Rac1-induced organization of actin and myosin during Drosophila larval wound healing. In: FEBS Letters. 2012 ; Vol. 586, No. 6. pp. 772-777.
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abstract = "Rho-family small GTPases regulate epithelial cell sheet migration by organizing actin and myosin during wound healing. Here, we report that Pak3, but not Pak1, is a downstream target protein for Rac1 in wound closure of the Drosophila larval epidermis. Pak3-deficient larvae failed to close a wound hole and this defect was not rescued by Pak1 expression, indicating differential functions of the two proteins. Pak3 localized to the wound margin, which selectively required Rac1. Pak3-deficient larvae showed severe defects in actin-myosin organization at the wound margin and in submarginal cells, which was reminiscent of the phenotypes of Rac1-deficient larvae. These results suggest that Pak3 specifically mediates Rac1 signaling in organizing actin and myosin during Drosophila epidermal wound healing.",
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Requirement for Pak3 in Rac1-induced organization of actin and myosin during Drosophila larval wound healing. / Baek, Seung Hee; Cho, Hae Weon; Kwon, Young Chang; Lee, Ji Hyun; Kim, Moon Jong; Lee, Hyangkyu; Choe, Kwang Min.

In: FEBS Letters, Vol. 586, No. 6, 23.03.2012, p. 772-777.

Research output: Contribution to journalArticle

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AU - Baek, Seung Hee

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AB - Rho-family small GTPases regulate epithelial cell sheet migration by organizing actin and myosin during wound healing. Here, we report that Pak3, but not Pak1, is a downstream target protein for Rac1 in wound closure of the Drosophila larval epidermis. Pak3-deficient larvae failed to close a wound hole and this defect was not rescued by Pak1 expression, indicating differential functions of the two proteins. Pak3 localized to the wound margin, which selectively required Rac1. Pak3-deficient larvae showed severe defects in actin-myosin organization at the wound margin and in submarginal cells, which was reminiscent of the phenotypes of Rac1-deficient larvae. These results suggest that Pak3 specifically mediates Rac1 signaling in organizing actin and myosin during Drosophila epidermal wound healing.

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