Requirement for Pak3 in Rac1-induced organization of actin and myosin during Drosophila larval wound healing

Seung Hee Baek, Hae Weon Cho, Young Chang Kwon, Ji Hyun Lee, Moon Jong Kim, Hyangkyu Lee, Kwang Min Choe

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15 Citations (Scopus)


Rho-family small GTPases regulate epithelial cell sheet migration by organizing actin and myosin during wound healing. Here, we report that Pak3, but not Pak1, is a downstream target protein for Rac1 in wound closure of the Drosophila larval epidermis. Pak3-deficient larvae failed to close a wound hole and this defect was not rescued by Pak1 expression, indicating differential functions of the two proteins. Pak3 localized to the wound margin, which selectively required Rac1. Pak3-deficient larvae showed severe defects in actin-myosin organization at the wound margin and in submarginal cells, which was reminiscent of the phenotypes of Rac1-deficient larvae. These results suggest that Pak3 specifically mediates Rac1 signaling in organizing actin and myosin during Drosophila epidermal wound healing.

Original languageEnglish
Pages (from-to)772-777
Number of pages6
JournalFEBS Letters
Issue number6
Publication statusPublished - 2012 Mar 23


All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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