Requirement of duplicated operons for maximal metabolism of phthalate by Rhodococcus sp. strain DK17

Ki Young Choi; Dockyu Kim; Jong Chan Chae; Gerben J. Zylstra; Eungbin Kim

doi:10.1016/j.bbrc.2007.04.009

Requirement of duplicated operons for maximal metabolism of phthalate by Rhodococcus sp. strain DK17

Ki Young Choi, Dockyu Kim, Jong Chan Chae, Gerben J. Zylstra, Eungbin Kim

Department of Systems Biology

Research output: Contribution to journal › Article

11 Citations (Scopus)

Abstract

The operons encoding the transformation of phthalate to protocatechuate are duplicated and present on two different megaplasmids [pDK2 (330 kb) and pDK3 (750 kb)] in Rhodococcus sp. strain DK17. RT-PCR experiments using gene-specific primers showed that both the pDK2- and the pDK3-encoded dihydroxyphthalate decarboxylase genes are simultaneously expressed during growth on phthalate. The doubling time of the pDK2-cured mutant strain DK176 in minimal liquid medium with 5 mM phthalate is 52.5% of that of the wild-type strain DK17. The data indicate that both copies of the phthalate operon are equally functional in DK17, and gene dosage is the main reason for slower growth of DK176 on phthalate.

Original language	English
Pages (from-to)	766-771
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	357
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2007.04.009
Publication status	Published - 2007 Jun 8

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All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Cite this

Choi, K. Y., Kim, D., Chae, J. C., Zylstra, G. J., & Kim, E. (2007). Requirement of duplicated operons for maximal metabolism of phthalate by Rhodococcus sp. strain DK17. Biochemical and Biophysical Research Communications, 357(3), 766-771. https://doi.org/10.1016/j.bbrc.2007.04.009

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Access to Document

10.1016/j.bbrc.2007.04.009