Requirement of duplicated operons for maximal metabolism of phthalate by Rhodococcus sp. strain DK17

Ki Young Choi, Dockyu Kim, Jong Chan Chae, Gerben J. Zylstra, Eungbin KIm

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The operons encoding the transformation of phthalate to protocatechuate are duplicated and present on two different megaplasmids [pDK2 (330 kb) and pDK3 (750 kb)] in Rhodococcus sp. strain DK17. RT-PCR experiments using gene-specific primers showed that both the pDK2- and the pDK3-encoded dihydroxyphthalate decarboxylase genes are simultaneously expressed during growth on phthalate. The doubling time of the pDK2-cured mutant strain DK176 in minimal liquid medium with 5 mM phthalate is 52.5% of that of the wild-type strain DK17. The data indicate that both copies of the phthalate operon are equally functional in DK17, and gene dosage is the main reason for slower growth of DK176 on phthalate.

Original languageEnglish
Pages (from-to)766-771
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume357
Issue number3
DOIs
Publication statusPublished - 2007 Jun 8

Fingerprint

Rhodococcus
Operon
Metabolism
Genes
Carboxy-Lyases
Gene Dosage
Growth
phthalic acid
Polymerase Chain Reaction
Liquids
Experiments

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Choi, Ki Young ; Kim, Dockyu ; Chae, Jong Chan ; Zylstra, Gerben J. ; KIm, Eungbin. / Requirement of duplicated operons for maximal metabolism of phthalate by Rhodococcus sp. strain DK17. In: Biochemical and Biophysical Research Communications. 2007 ; Vol. 357, No. 3. pp. 766-771.
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Requirement of duplicated operons for maximal metabolism of phthalate by Rhodococcus sp. strain DK17. / Choi, Ki Young; Kim, Dockyu; Chae, Jong Chan; Zylstra, Gerben J.; KIm, Eungbin.

In: Biochemical and Biophysical Research Communications, Vol. 357, No. 3, 08.06.2007, p. 766-771.

Research output: Contribution to journalArticle

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AB - The operons encoding the transformation of phthalate to protocatechuate are duplicated and present on two different megaplasmids [pDK2 (330 kb) and pDK3 (750 kb)] in Rhodococcus sp. strain DK17. RT-PCR experiments using gene-specific primers showed that both the pDK2- and the pDK3-encoded dihydroxyphthalate decarboxylase genes are simultaneously expressed during growth on phthalate. The doubling time of the pDK2-cured mutant strain DK176 in minimal liquid medium with 5 mM phthalate is 52.5% of that of the wild-type strain DK17. The data indicate that both copies of the phthalate operon are equally functional in DK17, and gene dosage is the main reason for slower growth of DK176 on phthalate.

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