Abstract
The operons encoding the transformation of phthalate to protocatechuate are duplicated and present on two different megaplasmids [pDK2 (330 kb) and pDK3 (750 kb)] in Rhodococcus sp. strain DK17. RT-PCR experiments using gene-specific primers showed that both the pDK2- and the pDK3-encoded dihydroxyphthalate decarboxylase genes are simultaneously expressed during growth on phthalate. The doubling time of the pDK2-cured mutant strain DK176 in minimal liquid medium with 5 mM phthalate is 52.5% of that of the wild-type strain DK17. The data indicate that both copies of the phthalate operon are equally functional in DK17, and gene dosage is the main reason for slower growth of DK176 on phthalate.
| Original language | English |
|---|---|
| Pages (from-to) | 766-771 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 357 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2007 Jun 8 |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
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Requirement of duplicated operons for maximal metabolism of phthalate by Rhodococcus sp. strain DK17. / Choi, Ki Young; Kim, Dockyu; Chae, Jong Chan; Zylstra, Gerben J.; KIm, Eungbin.
In: Biochemical and Biophysical Research Communications, Vol. 357, No. 3, 08.06.2007, p. 766-771.Research output: Contribution to journal › Article
TY - JOUR
T1 - Requirement of duplicated operons for maximal metabolism of phthalate by Rhodococcus sp. strain DK17
AU - Choi, Ki Young
AU - Kim, Dockyu
AU - Chae, Jong Chan
AU - Zylstra, Gerben J.
AU - KIm, Eungbin
PY - 2007/6/8
Y1 - 2007/6/8
N2 - The operons encoding the transformation of phthalate to protocatechuate are duplicated and present on two different megaplasmids [pDK2 (330 kb) and pDK3 (750 kb)] in Rhodococcus sp. strain DK17. RT-PCR experiments using gene-specific primers showed that both the pDK2- and the pDK3-encoded dihydroxyphthalate decarboxylase genes are simultaneously expressed during growth on phthalate. The doubling time of the pDK2-cured mutant strain DK176 in minimal liquid medium with 5 mM phthalate is 52.5% of that of the wild-type strain DK17. The data indicate that both copies of the phthalate operon are equally functional in DK17, and gene dosage is the main reason for slower growth of DK176 on phthalate.
AB - The operons encoding the transformation of phthalate to protocatechuate are duplicated and present on two different megaplasmids [pDK2 (330 kb) and pDK3 (750 kb)] in Rhodococcus sp. strain DK17. RT-PCR experiments using gene-specific primers showed that both the pDK2- and the pDK3-encoded dihydroxyphthalate decarboxylase genes are simultaneously expressed during growth on phthalate. The doubling time of the pDK2-cured mutant strain DK176 in minimal liquid medium with 5 mM phthalate is 52.5% of that of the wild-type strain DK17. The data indicate that both copies of the phthalate operon are equally functional in DK17, and gene dosage is the main reason for slower growth of DK176 on phthalate.
UR - http://www.scopus.com/inward/record.url?scp=34247569068&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34247569068&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2007.04.009
DO - 10.1016/j.bbrc.2007.04.009
M3 - Article
C2 - 17449009
AN - SCOPUS:34247569068
VL - 357
SP - 766
EP - 771
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -