Reserpine treatment activates AMP activated protein kinase (AMPK)

Rackhyun Park, Kang Il Lee, Hyunju Kim, Minsu Jang, Thi Kim Quy Ha, Won Keun Oh, Junsoo Park

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Reserpine is a well-known medicine for the treatment of hypertension, however the role of reserpine in cell signaling is not fully understood. Here, we report that reserpine treatment induces the phosphorylation of AMP activated protein kinase (AMPK) at threonine 172 (T172) in PC12 cells. Phosphorylation of AMPK T172 is regulated by upstream signaling molecules, and the increase of phospho-T172 indicates that AMPK is activated. When we examined the FOXO3a dependent transcription by using the FHRE-Luc reporter assay, reserpine treatment repressed the FHRE-Luc reporter activity in a dose dependent manner. Finally, we showed that reserpine treatment induced the phosphorylation of AMPK as well as cell death in MCF-7 cells. These results suggest that AMPK is a potential cellular target of reserpine.

Original languageEnglish
Pages (from-to)157-161
Number of pages5
JournalNatural Product Sciences
Volume23
Issue number3
DOIs
Publication statusPublished - 2017 Jan 1

Fingerprint

AMP-Activated Protein Kinases
Reserpine
Phosphorylation
Threonine
Cell signaling
PC12 Cells
MCF-7 Cells
Cell death
Transcription
Medicine
Assays
Cell Death
Hypertension
Molecules

All Science Journal Classification (ASJC) codes

  • Drug Discovery
  • Organic Chemistry

Cite this

Park, Rackhyun ; Lee, Kang Il ; Kim, Hyunju ; Jang, Minsu ; Ha, Thi Kim Quy ; Oh, Won Keun ; Park, Junsoo. / Reserpine treatment activates AMP activated protein kinase (AMPK). In: Natural Product Sciences. 2017 ; Vol. 23, No. 3. pp. 157-161.
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Reserpine treatment activates AMP activated protein kinase (AMPK). / Park, Rackhyun; Lee, Kang Il; Kim, Hyunju; Jang, Minsu; Ha, Thi Kim Quy; Oh, Won Keun; Park, Junsoo.

In: Natural Product Sciences, Vol. 23, No. 3, 01.01.2017, p. 157-161.

Research output: Contribution to journalArticle

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AU - Park, Rackhyun

AU - Lee, Kang Il

AU - Kim, Hyunju

AU - Jang, Minsu

AU - Ha, Thi Kim Quy

AU - Oh, Won Keun

AU - Park, Junsoo

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AB - Reserpine is a well-known medicine for the treatment of hypertension, however the role of reserpine in cell signaling is not fully understood. Here, we report that reserpine treatment induces the phosphorylation of AMP activated protein kinase (AMPK) at threonine 172 (T172) in PC12 cells. Phosphorylation of AMPK T172 is regulated by upstream signaling molecules, and the increase of phospho-T172 indicates that AMPK is activated. When we examined the FOXO3a dependent transcription by using the FHRE-Luc reporter assay, reserpine treatment repressed the FHRE-Luc reporter activity in a dose dependent manner. Finally, we showed that reserpine treatment induced the phosphorylation of AMPK as well as cell death in MCF-7 cells. These results suggest that AMPK is a potential cellular target of reserpine.

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