Restoration of heat shock protein70 suppresses gastric mucosal inducible nitric oxide synthase expression induced by Helicobacter pylori

Marie Yeo, Hyun Kyung Park, Dong Kyu Kim, Won Cho Sung, Suk Kim Young, Yun Cho Sang, Young-Ki Paik, Ki Baik Hahm

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Heat shock proteins (HSPs) are crucial for the maintenance of cell integrity during normal cellular growth as well as during pathophysiological conditions. While functioning mainly as molecular chaperones, HSPs also appear to be involved in diverse biological activities, such as apoptosis, carcinogenesis, and cytoprotection from cytotoxic damage. Infection with Helicobacter pylori causes inflammation in the gastric mucosa, leading to gastritis, gastric ulcers, duodenal ulcer disease, and even gastric cancer, but the role of HSPs in H. pylori-associated gastropathy is not known. Using two-dimensional electrophoretic analysis, we have observed significant shifts in HSP profiles after H. pylori infection in RGM-1 cells. We therefore evaluated the effect of treatments that induce HSPs on H. pylori-induced inducible nitric oxide synthase (iNOS) expression. We found that H. pylori infection significantly attenuated the expression of HSP70, whereas exposure of cells to noncytotoxic heat shock or geranylgeranylacetone restored HSP70 expression, as well as suppressing the expression of iNOS, a major cause of H. pylori-induced gastric tissue damage. Our results suggest that induction of HSP70 confers cytoprotection against H. pylori infection by inhibiting the expression of iNOS. In conclusion, these results provide important insights into the flux in HSPs profiles in response to H. pylori infection and highlight the cytoprotective role of HSP70 in H. pylori infection.

Original languageEnglish
Pages (from-to)3335-3342
Number of pages8
JournalProteomics
Volume4
Issue number11
DOIs
Publication statusPublished - 2004 Nov 1

Fingerprint

Nitric Oxide Synthase Type II
Heat-Shock Proteins
Helicobacter pylori
Restoration
Shock
Stomach
Hot Temperature
Helicobacter Infections
geranylgeranylacetone
Cytoprotection
Molecular Chaperones
Bioactivity
Duodenal Diseases
Gastritis
Stomach Ulcer
Tissue
Apoptosis
Duodenal Ulcer
Fluxes
Gastric Mucosa

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Yeo, Marie ; Park, Hyun Kyung ; Kim, Dong Kyu ; Sung, Won Cho ; Young, Suk Kim ; Sang, Yun Cho ; Paik, Young-Ki ; Hahm, Ki Baik. / Restoration of heat shock protein70 suppresses gastric mucosal inducible nitric oxide synthase expression induced by Helicobacter pylori. In: Proteomics. 2004 ; Vol. 4, No. 11. pp. 3335-3342.
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Restoration of heat shock protein70 suppresses gastric mucosal inducible nitric oxide synthase expression induced by Helicobacter pylori. / Yeo, Marie; Park, Hyun Kyung; Kim, Dong Kyu; Sung, Won Cho; Young, Suk Kim; Sang, Yun Cho; Paik, Young-Ki; Hahm, Ki Baik.

In: Proteomics, Vol. 4, No. 11, 01.11.2004, p. 3335-3342.

Research output: Contribution to journalArticle

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