Retinoblastoma protein regulates cell proliferation, differentiation, and endoreduplication in plants

Jong A. Park, Joon Woo Ahn, Yu Kyung Kim, Jung Kim Su, Ju Kon Kim, Woo Taek Kim, Hyunsook Pai

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Retinoblastoma protein (Rb) plays a key role in cell cycle control, cell differentiation, and apoptosis in animals. In this study, we used virus-induced gene silencing (VIGS) to investigate the cellular functions of Rb in higher plants. VIGS of NbRBR1, which encodes the Nicotiana benthamiana Rb homolog, resulted in growth retardation and abnormal organ development. At the cellular level, Rb suppression caused prolonged cell proliferation in tissues that are normally differentiated, which indicates that Rb is a negative regulator of plant cell division. Furthermore, differentiation of the epidermal pavement cells and trichomes was partially retarded, and stomatal clusters formed in the epidermis, likely due to uncontrolled cell division of stomata precursor cells. Rb suppression also caused extra DNA replication in endoreduplicating leaf cells, suggesting a role of Rb in the endocycle. These Rb phenotypes were accompanied by stimulated transcription of E2F and E2F-regulated S-phase genes. Thus, disruption of Rb function in plants leads to ectopic cell division in major organs that correlates with a delay in cell differentiation as well as increased endoreduplication, which indicates that Rb coordinates these processes in plant organ development.

Original languageEnglish
Pages (from-to)153-163
Number of pages11
JournalPlant Journal
Volume42
Issue number2
DOIs
Publication statusPublished - 2005 Apr 1

Fingerprint

Endoreduplication
endopolyploidy
Retinoblastoma Protein
Cell Division
cell division
Cell Differentiation
cell proliferation
Cell Proliferation
Gene Silencing
gene silencing
cell differentiation
Plant Organogenesis
Trichomes
Viruses
viruses
proteins
Nicotiana benthamiana
Plant Cells
DNA replication
cells

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Park, Jong A. ; Ahn, Joon Woo ; Kim, Yu Kyung ; Su, Jung Kim ; Kim, Ju Kon ; Kim, Woo Taek ; Pai, Hyunsook. / Retinoblastoma protein regulates cell proliferation, differentiation, and endoreduplication in plants. In: Plant Journal. 2005 ; Vol. 42, No. 2. pp. 153-163.
@article{e675af5371e54792a0038bdeac46d7c6,
title = "Retinoblastoma protein regulates cell proliferation, differentiation, and endoreduplication in plants",
abstract = "Retinoblastoma protein (Rb) plays a key role in cell cycle control, cell differentiation, and apoptosis in animals. In this study, we used virus-induced gene silencing (VIGS) to investigate the cellular functions of Rb in higher plants. VIGS of NbRBR1, which encodes the Nicotiana benthamiana Rb homolog, resulted in growth retardation and abnormal organ development. At the cellular level, Rb suppression caused prolonged cell proliferation in tissues that are normally differentiated, which indicates that Rb is a negative regulator of plant cell division. Furthermore, differentiation of the epidermal pavement cells and trichomes was partially retarded, and stomatal clusters formed in the epidermis, likely due to uncontrolled cell division of stomata precursor cells. Rb suppression also caused extra DNA replication in endoreduplicating leaf cells, suggesting a role of Rb in the endocycle. These Rb phenotypes were accompanied by stimulated transcription of E2F and E2F-regulated S-phase genes. Thus, disruption of Rb function in plants leads to ectopic cell division in major organs that correlates with a delay in cell differentiation as well as increased endoreduplication, which indicates that Rb coordinates these processes in plant organ development.",
author = "Park, {Jong A.} and Ahn, {Joon Woo} and Kim, {Yu Kyung} and Su, {Jung Kim} and Kim, {Ju Kon} and Kim, {Woo Taek} and Hyunsook Pai",
year = "2005",
month = "4",
day = "1",
doi = "10.1111/j.1365-313X.2005.02361.x",
language = "English",
volume = "42",
pages = "153--163",
journal = "Plant Journal",
issn = "0960-7412",
publisher = "Wiley-Blackwell",
number = "2",

}

Retinoblastoma protein regulates cell proliferation, differentiation, and endoreduplication in plants. / Park, Jong A.; Ahn, Joon Woo; Kim, Yu Kyung; Su, Jung Kim; Kim, Ju Kon; Kim, Woo Taek; Pai, Hyunsook.

In: Plant Journal, Vol. 42, No. 2, 01.04.2005, p. 153-163.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Retinoblastoma protein regulates cell proliferation, differentiation, and endoreduplication in plants

AU - Park, Jong A.

AU - Ahn, Joon Woo

AU - Kim, Yu Kyung

AU - Su, Jung Kim

AU - Kim, Ju Kon

AU - Kim, Woo Taek

AU - Pai, Hyunsook

PY - 2005/4/1

Y1 - 2005/4/1

N2 - Retinoblastoma protein (Rb) plays a key role in cell cycle control, cell differentiation, and apoptosis in animals. In this study, we used virus-induced gene silencing (VIGS) to investigate the cellular functions of Rb in higher plants. VIGS of NbRBR1, which encodes the Nicotiana benthamiana Rb homolog, resulted in growth retardation and abnormal organ development. At the cellular level, Rb suppression caused prolonged cell proliferation in tissues that are normally differentiated, which indicates that Rb is a negative regulator of plant cell division. Furthermore, differentiation of the epidermal pavement cells and trichomes was partially retarded, and stomatal clusters formed in the epidermis, likely due to uncontrolled cell division of stomata precursor cells. Rb suppression also caused extra DNA replication in endoreduplicating leaf cells, suggesting a role of Rb in the endocycle. These Rb phenotypes were accompanied by stimulated transcription of E2F and E2F-regulated S-phase genes. Thus, disruption of Rb function in plants leads to ectopic cell division in major organs that correlates with a delay in cell differentiation as well as increased endoreduplication, which indicates that Rb coordinates these processes in plant organ development.

AB - Retinoblastoma protein (Rb) plays a key role in cell cycle control, cell differentiation, and apoptosis in animals. In this study, we used virus-induced gene silencing (VIGS) to investigate the cellular functions of Rb in higher plants. VIGS of NbRBR1, which encodes the Nicotiana benthamiana Rb homolog, resulted in growth retardation and abnormal organ development. At the cellular level, Rb suppression caused prolonged cell proliferation in tissues that are normally differentiated, which indicates that Rb is a negative regulator of plant cell division. Furthermore, differentiation of the epidermal pavement cells and trichomes was partially retarded, and stomatal clusters formed in the epidermis, likely due to uncontrolled cell division of stomata precursor cells. Rb suppression also caused extra DNA replication in endoreduplicating leaf cells, suggesting a role of Rb in the endocycle. These Rb phenotypes were accompanied by stimulated transcription of E2F and E2F-regulated S-phase genes. Thus, disruption of Rb function in plants leads to ectopic cell division in major organs that correlates with a delay in cell differentiation as well as increased endoreduplication, which indicates that Rb coordinates these processes in plant organ development.

UR - http://www.scopus.com/inward/record.url?scp=17844380018&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=17844380018&partnerID=8YFLogxK

U2 - 10.1111/j.1365-313X.2005.02361.x

DO - 10.1111/j.1365-313X.2005.02361.x

M3 - Article

VL - 42

SP - 153

EP - 163

JO - Plant Journal

JF - Plant Journal

SN - 0960-7412

IS - 2

ER -