Retinoic acid modulates chondrogenesis in the developing mouse cranial base

Hyuk Jae Kwon, Jeong Oh Shin, Jong Min Lee, Kyoung Won Cho, Min Jung Lee, Sung Won Cho, Hansung Jung

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The retinoic acid (RA) signaling pathway is known to play important roles during craniofacial development and skeletogenesis. However, the specific mechanism involving RA in cranial base development has not yet been clearly described. This study investigated how RA modulates endochondral bone development of the cranial base by monitoring the RA receptor RARγ, BMP4, and markers of proliferation, programmed cell death, chondrogenesis, and osteogenesis. We first examined the dynamic morphological and molecular changes in the sphenooccipital synchondrosis-forming region in the mouse embryo cranial bases at E12-E16. In vitro organ cultures employing beads soaked in RA and retinoid-signaling inhibitor citral were compared. In the RA study, the sphenooccipital synchondrosis showed reduced cartilage matrix and lower BMP4 expression while hypertrophic chondrocytes were replaced with proliferating chondrocytes. Retardation of chondrocyte hypertrophy was exhibited in citral-treated specimens, while BMP4 expression was slightly increased and programmed cell death was induced within the sphenooccipital synchondrosis. Our results demonstrate that RA modulates chondrocytes to proliferate, differentiate, or undergo programmed cell death during endochondral bone formation in the developing cranial base.

Original languageEnglish
Pages (from-to)574-583
Number of pages10
JournalJournal of Experimental Zoology Part B: Molecular and Developmental Evolution
Volume316 B
Issue number8
DOIs
Publication statusPublished - 2011 Dec 15

Fingerprint

chondrogenesis
Chondrogenesis
retinoic acid
Skull Base
Tretinoin
citral
Chondrocytes
chondrocytes
acid
mice
Cell Death
apoptosis
Osteogenesis
bone formation
bone
Retinoic Acid Receptors
Organ Culture Techniques
Bone Development
Retinoids
Molecular Dynamics Simulation

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Medicine
  • Animal Science and Zoology
  • Genetics
  • Developmental Biology

Cite this

Kwon, Hyuk Jae ; Shin, Jeong Oh ; Lee, Jong Min ; Cho, Kyoung Won ; Lee, Min Jung ; Cho, Sung Won ; Jung, Hansung. / Retinoic acid modulates chondrogenesis in the developing mouse cranial base. In: Journal of Experimental Zoology Part B: Molecular and Developmental Evolution. 2011 ; Vol. 316 B, No. 8. pp. 574-583.
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Retinoic acid modulates chondrogenesis in the developing mouse cranial base. / Kwon, Hyuk Jae; Shin, Jeong Oh; Lee, Jong Min; Cho, Kyoung Won; Lee, Min Jung; Cho, Sung Won; Jung, Hansung.

In: Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, Vol. 316 B, No. 8, 15.12.2011, p. 574-583.

Research output: Contribution to journalArticle

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