Reusable surface amplified nanobiosensor for the sub PFU/mL level detection of airborne virus

Junghyun Shin, Hyeong Rae Kim, Pan Kee Bae, Haneul Yoo, Jeongsu Kim, Yoonji Choi, Yong Beom Shin, Jungho Hwang, Seunghun Hong

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


We developed a reusable surface-amplified nanobiosensor for monitoring airborne viruses with a sub-PFU/mL level detection limit. Here, sandwich structures consisted of magnetic particles functionalized with antibodies, target viruses, and alkaline phosphatases (ALPs) were formed, and they were magnetically concentrated on Ni patterns near an electrochemical sensor transducer. Then, the electrical signals from electrochemical markers generated by ALPs were measured with the sensor transducer, enabling highly-sensitive virus detection. The sandwich structures in the used sensor chip could be removed by applying an external magnetic field, and we could reuse the sensor transducer chip. As a proof of concepts, the repeated detection of airborne influenza virus using a single sensor chip was demonstrated with a detection limit down to a sub-PFU/mL level. Using a single reusable sensor transducer chip, the hemagglutinin (HA) of influenza A (H1N1) virus with different concentrations were measured down to 10 aM level. Importantly, our sensor chip exhibited reliable sensing signals even after more than 18 times of the repeated HA sensing measurements. Furthermore, airborne influenza viruses collected from the air could be measured down to 0.01 PFU/mL level. Interestingly, the detailed quantitative analysis of the measurement results revealed the degradation of HA proteins on the viruses after the air exposure. Considering the ultrasensitivity and reusability of our sensors, it can provide a powerful tool to help preventing epidemics by airborne pathogens in the future.

Original languageEnglish
Article number16776
JournalScientific reports
Issue number1
Publication statusPublished - 2021 Dec

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (MSIT) of Korea (No. 2013M3A6B2078961, 2013M3A6B2078950 and 2020R1A2B5B02002152). S.H. would like to acknowledge the support from the Ministry of Trade, Industry & Energy (MOTIE, Korea) (20012390), Samsung Electronics Co. Ltd. (No. 201209-07908-01) and the European Research Council (ERC) under the European Union’s Horizon 2020 programme (Grant Agreement No. 682286). J.H. would like to acknowledge the support from the Korea Environment Industry & Technology Institute (KEITI) through Technology Development Project for Biological Hazards Management in Indoor Air Program funded by Korea Ministry of Environment (MOE) (G232021010381).

Publisher Copyright:
© 2021, The Author(s).

All Science Journal Classification (ASJC) codes

  • General


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