Reversible Cell Layering for Heterogeneous Cell Assembly Mediated by Ionic Cross-Linking of Chitosan and a Functionalized Cell Surface Membrane

Seungmi Ryu, Hyunbum Kim, Seokyung Kang, Kwangsoo Shin, Seon Yeop Jung, Jiwoong Heo, Jin Han, Jeong Kee Yoon, Ju Ro Lee, Jinkee Hong, Kyung Hyun Ahn, Taeghwan Hyeon, Nathaniel Suk Yeon Hwang, Byung Soo Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Current heterogeneous cell assembly techniques in coculture systems rely on irreversible cell layering or a cell separation membrane. However, the techniques possess major drawbacks of inefficiency in direct interactions of the assembled cell layers and cell separation following coculture, which hamper characterization and therapeutic applications of the cells following coculture. Here, we develop a reversible cell layering platform for assembly of heterogeneous cells that allows both active direct cell-cell interactions and facile cell separation. Anionic maleimide-chondroitin-sulfate is grafted onto the surface membrane of myogenic C2C12 cells and human mesenchymal stem cells (hMSCs) to modify the surface charge of the cells without cytotoxicity. A highly porous chitosan thin film is formed in situ interspacing between the heterogeneous cell layers via ionic cross-linking of cationic chitosan and anionic functionalized cells, forming compactly assembled double-layered cell constructs. The chitosan film enables layering of the cells, which allows active direct interactions between the cell layers, and facile delayering of the cells through simple treatment with mild shear stress. The developed platform promotes the myogenic commitment of hMSCs via direct contact with C2C12 cells, mimicking the interactions that trigger stem cell differentiation in vivo. Delivery of the myogenic committed cells to muscle-injured animal models shows evident muscle regeneration.

Original languageEnglish
Pages (from-to)5294-5305
Number of pages12
JournalChemistry of Materials
Volume29
Issue number12
DOIs
Publication statusPublished - 2017 Jun 27

Fingerprint

Chitosan
Stem cells
Cells
Membranes
Muscle
Chondroitin Sulfates
Cytotoxicity
Surface charge
Shear stress
Animals
Thin films

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)
  • Materials Chemistry

Cite this

Ryu, Seungmi ; Kim, Hyunbum ; Kang, Seokyung ; Shin, Kwangsoo ; Jung, Seon Yeop ; Heo, Jiwoong ; Han, Jin ; Yoon, Jeong Kee ; Lee, Ju Ro ; Hong, Jinkee ; Ahn, Kyung Hyun ; Hyeon, Taeghwan ; Hwang, Nathaniel Suk Yeon ; Kim, Byung Soo. / Reversible Cell Layering for Heterogeneous Cell Assembly Mediated by Ionic Cross-Linking of Chitosan and a Functionalized Cell Surface Membrane. In: Chemistry of Materials. 2017 ; Vol. 29, No. 12. pp. 5294-5305.
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abstract = "Current heterogeneous cell assembly techniques in coculture systems rely on irreversible cell layering or a cell separation membrane. However, the techniques possess major drawbacks of inefficiency in direct interactions of the assembled cell layers and cell separation following coculture, which hamper characterization and therapeutic applications of the cells following coculture. Here, we develop a reversible cell layering platform for assembly of heterogeneous cells that allows both active direct cell-cell interactions and facile cell separation. Anionic maleimide-chondroitin-sulfate is grafted onto the surface membrane of myogenic C2C12 cells and human mesenchymal stem cells (hMSCs) to modify the surface charge of the cells without cytotoxicity. A highly porous chitosan thin film is formed in situ interspacing between the heterogeneous cell layers via ionic cross-linking of cationic chitosan and anionic functionalized cells, forming compactly assembled double-layered cell constructs. The chitosan film enables layering of the cells, which allows active direct interactions between the cell layers, and facile delayering of the cells through simple treatment with mild shear stress. The developed platform promotes the myogenic commitment of hMSCs via direct contact with C2C12 cells, mimicking the interactions that trigger stem cell differentiation in vivo. Delivery of the myogenic committed cells to muscle-injured animal models shows evident muscle regeneration.",
author = "Seungmi Ryu and Hyunbum Kim and Seokyung Kang and Kwangsoo Shin and Jung, {Seon Yeop} and Jiwoong Heo and Jin Han and Yoon, {Jeong Kee} and Lee, {Ju Ro} and Jinkee Hong and Ahn, {Kyung Hyun} and Taeghwan Hyeon and Hwang, {Nathaniel Suk Yeon} and Kim, {Byung Soo}",
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Ryu, S, Kim, H, Kang, S, Shin, K, Jung, SY, Heo, J, Han, J, Yoon, JK, Lee, JR, Hong, J, Ahn, KH, Hyeon, T, Hwang, NSY & Kim, BS 2017, 'Reversible Cell Layering for Heterogeneous Cell Assembly Mediated by Ionic Cross-Linking of Chitosan and a Functionalized Cell Surface Membrane', Chemistry of Materials, vol. 29, no. 12, pp. 5294-5305. https://doi.org/10.1021/acs.chemmater.7b01484

Reversible Cell Layering for Heterogeneous Cell Assembly Mediated by Ionic Cross-Linking of Chitosan and a Functionalized Cell Surface Membrane. / Ryu, Seungmi; Kim, Hyunbum; Kang, Seokyung; Shin, Kwangsoo; Jung, Seon Yeop; Heo, Jiwoong; Han, Jin; Yoon, Jeong Kee; Lee, Ju Ro; Hong, Jinkee; Ahn, Kyung Hyun; Hyeon, Taeghwan; Hwang, Nathaniel Suk Yeon; Kim, Byung Soo.

In: Chemistry of Materials, Vol. 29, No. 12, 27.06.2017, p. 5294-5305.

Research output: Contribution to journalArticle

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AU - Ryu, Seungmi

AU - Kim, Hyunbum

AU - Kang, Seokyung

AU - Shin, Kwangsoo

AU - Jung, Seon Yeop

AU - Heo, Jiwoong

AU - Han, Jin

AU - Yoon, Jeong Kee

AU - Lee, Ju Ro

AU - Hong, Jinkee

AU - Ahn, Kyung Hyun

AU - Hyeon, Taeghwan

AU - Hwang, Nathaniel Suk Yeon

AU - Kim, Byung Soo

PY - 2017/6/27

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N2 - Current heterogeneous cell assembly techniques in coculture systems rely on irreversible cell layering or a cell separation membrane. However, the techniques possess major drawbacks of inefficiency in direct interactions of the assembled cell layers and cell separation following coculture, which hamper characterization and therapeutic applications of the cells following coculture. Here, we develop a reversible cell layering platform for assembly of heterogeneous cells that allows both active direct cell-cell interactions and facile cell separation. Anionic maleimide-chondroitin-sulfate is grafted onto the surface membrane of myogenic C2C12 cells and human mesenchymal stem cells (hMSCs) to modify the surface charge of the cells without cytotoxicity. A highly porous chitosan thin film is formed in situ interspacing between the heterogeneous cell layers via ionic cross-linking of cationic chitosan and anionic functionalized cells, forming compactly assembled double-layered cell constructs. The chitosan film enables layering of the cells, which allows active direct interactions between the cell layers, and facile delayering of the cells through simple treatment with mild shear stress. The developed platform promotes the myogenic commitment of hMSCs via direct contact with C2C12 cells, mimicking the interactions that trigger stem cell differentiation in vivo. Delivery of the myogenic committed cells to muscle-injured animal models shows evident muscle regeneration.

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