RGD peptide and graphene oxide co-functionalized PLGA nanofiber scaffolds for vascular tissue engineering

Yong Cheol Shin, Jeonghyo Kim, Sung Eun Kim, Su Jin Song, Suck Won Hong, Jin Woo Oh, Jaebeom Lee, Jong Chul Park, Suong Hyu Hyon, Dong Wook Han

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

In recent years, much research has been suggested and examined for the development of tissue engineering scaffolds to promote cellular behaviors. In our study, RGD peptide and graphene oxide (GO) co-functionalized poly(lactide-co-glycolide, PLGA) (RGD-GO-PLGA) nanofiber mats were fabricated via electrospinning, and their physicochemical and thermal properties were characterized to explore their potential as biofunctional scaffolds for vascular tissue engineering. Scanning electron microscopy images revealed that the RGD-GO-PLGA nanofiber mats were readily fabricated and composed of randomoriented electrospun nanofibers with average diameter of 558nm. The successful co-functionalization of RGD peptide and GO into the PLGA nanofibers was confirmed by Fourier-transform infrared spectroscopic analysis. Moreover, the surface hydrophilicity of the nanofiber mats was markedly increased by co-functionalizing with RGD peptide and GO. It was found that the mats were thermally stable under the cell culture condition. Furthermore, the initial attachment and proliferation of primarily cultured vascular smoothmuscle cells (VSMCs) on the RGD-GO-PLGA nanofibermats were evaluated. It was revealed that the RGD-GO-PLGA nanofibermats can effectively promote the growth of VSMCs. In conclusion, our findings suggest that the RGD-GO-PLGA nanofiber mats can be promising candidates for tissue engineering scaffolds effective for the regeneration of vascular smoothmuscle.

Original languageEnglish
Pages (from-to)159-166
Number of pages8
JournalRegenerative Biomaterials
Volume4
Issue number3
DOIs
Publication statusPublished - 2017 Jun 1

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Biomaterials

Cite this

Shin, Y. C., Kim, J., Kim, S. E., Song, S. J., Hong, S. W., Oh, J. W., Lee, J., Park, J. C., Hyon, S. H., & Han, D. W. (2017). RGD peptide and graphene oxide co-functionalized PLGA nanofiber scaffolds for vascular tissue engineering. Regenerative Biomaterials, 4(3), 159-166. https://doi.org/10.1093/rb/rbx001