Rhizosphere microbiome structure alters to enable wilt resistance in tomato

Min Jung Kwak, Hyun Gi Kong, Kihyuck Choi, Soon Kyeong Kwon, Ju Yeon Song, Jidam Lee, Pyeong An Lee, Soo Yeon Choi, Minseok Seo, Hyoung Ju Lee, Eun Joo Jung, Hyein Park, Nazish Roy, Heebal Kim, Myeong Min Lee, Edward M. Rubin, Seon Woo Lee, Jihyun F. Kim

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Tomato variety Hawaii 7996 is resistant to the soil-borne pathogen Ralstonia solanacearum, whereas the Moneymaker variety is susceptible to the pathogen. To evaluate whether plant-associated microorganisms have a role in disease resistance, we analyzed the rhizosphere microbiomes of both varieties in a mesocosm experiment. Microbiome structures differed between the two cultivars. Transplantation of rhizosphere microbiota from resistant plants suppressed disease symptoms in susceptible plants. Comparative analyses of rhizosphere metagenomes from resistant and susceptible plants enabled the identification and assembly of a flavobacterial genome that was far more abundant in the resistant plant rhizosphere microbiome than in that of the susceptible plant. We cultivated this flavobacterium, named TRM1, and found that it could suppress R. solanacearum-disease development in a susceptible plant in pot experiments. Our findings reveal a role for native microbiota in protecting plants from microbial pathogens, and our approach charts a path toward the development of probiotics to ameliorate plant diseases.

Original languageEnglish
Pages (from-to)1100-1116
Number of pages17
JournalNature Biotechnology
Volume36
Issue number11
DOIs
Publication statusPublished - 2018 Dec 1

Fingerprint

Rhizosphere
Microbiota
Lycopersicon esculentum
Pathogens
Ralstonia solanacearum
Plant Diseases
Microorganisms
Metagenome
Flavobacterium
Genes
Experiments
Disease Resistance
Soils
Probiotics
Soil
Transplantation
Genome

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

Cite this

Kwak, M. J., Kong, H. G., Choi, K., Kwon, S. K., Song, J. Y., Lee, J., ... Kim, J. F. (2018). Rhizosphere microbiome structure alters to enable wilt resistance in tomato. Nature Biotechnology, 36(11), 1100-1116. https://doi.org/10.1038/nbt.4232
Kwak, Min Jung ; Kong, Hyun Gi ; Choi, Kihyuck ; Kwon, Soon Kyeong ; Song, Ju Yeon ; Lee, Jidam ; Lee, Pyeong An ; Choi, Soo Yeon ; Seo, Minseok ; Lee, Hyoung Ju ; Jung, Eun Joo ; Park, Hyein ; Roy, Nazish ; Kim, Heebal ; Lee, Myeong Min ; Rubin, Edward M. ; Lee, Seon Woo ; Kim, Jihyun F. / Rhizosphere microbiome structure alters to enable wilt resistance in tomato. In: Nature Biotechnology. 2018 ; Vol. 36, No. 11. pp. 1100-1116.
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Kwak, MJ, Kong, HG, Choi, K, Kwon, SK, Song, JY, Lee, J, Lee, PA, Choi, SY, Seo, M, Lee, HJ, Jung, EJ, Park, H, Roy, N, Kim, H, Lee, MM, Rubin, EM, Lee, SW & Kim, JF 2018, 'Rhizosphere microbiome structure alters to enable wilt resistance in tomato', Nature Biotechnology, vol. 36, no. 11, pp. 1100-1116. https://doi.org/10.1038/nbt.4232

Rhizosphere microbiome structure alters to enable wilt resistance in tomato. / Kwak, Min Jung; Kong, Hyun Gi; Choi, Kihyuck; Kwon, Soon Kyeong; Song, Ju Yeon; Lee, Jidam; Lee, Pyeong An; Choi, Soo Yeon; Seo, Minseok; Lee, Hyoung Ju; Jung, Eun Joo; Park, Hyein; Roy, Nazish; Kim, Heebal; Lee, Myeong Min; Rubin, Edward M.; Lee, Seon Woo; Kim, Jihyun F.

In: Nature Biotechnology, Vol. 36, No. 11, 01.12.2018, p. 1100-1116.

Research output: Contribution to journalArticle

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AU - Kim, Heebal

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Kwak MJ, Kong HG, Choi K, Kwon SK, Song JY, Lee J et al. Rhizosphere microbiome structure alters to enable wilt resistance in tomato. Nature Biotechnology. 2018 Dec 1;36(11):1100-1116. https://doi.org/10.1038/nbt.4232