RhoB induces apoptosis via direct interaction with TNFAIP1 in HeLa cells

Dong Myung Kim, Kyung Sook Chung, Shin Jung Choi, Yu Jin Jung, Song Kyu Park, Gyoon Hee Han, Jae Seok Ha, Kyung Bin Song, Nam Song Choi, Hwan Mook Kim, Misun Won, Yeon Soo Seo

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

RhoB, a tumor suppressor, has emerged as an interesting cancer target, and extensive studies aimed at understanding its role in apoptosis have been performed. In our study, we investigated the involvement of RhoB-interacting molecules in apoptosis. To identify RhoB-interacting proteins, we performed yeast-two hybrid screening assays using RhoB as a bait and isolated TNFAIP1, a TNFα-induced protein containing the BTB/POZ domain. The interaction between RhoB and TNFAIP1 was demonstrated in vivo through coimmunoprecipitation studies and in vitro binding assays. RFP-TNFAIP1 was found to be partially colocalized with EGFP-RhoB. The partial colocalization of RhoB and TNFAIP1 in endosomes suggests that RhoB-TNFAIP1 interactions may have a functional role in apoptosis. TNFAIP1 elicited proapoptotic activity, while simultaneous expression of RhoB and TNFAIP1 resulted in a dramatic increase in apoptosis in HeLa cells. Furthermore, knockdown of RhoB using siRNA clearly rescued cells from apoptosis induced by TNFAIP1. This finding suggests that interactions between RhoB and TNFAIP1 are crucial for induction of apoptosis in HeLa cells. The observation of increased SAPK/JNK phosphorylation in apoptotic cells and the finding that a JNK inhibitor suppressed apoptosis indicates that SAPK/JNK signaling may be involved in apoptosis induced by RhoB-TNFAIP1 interactions. In conclusion, we found that RhoB interacts with TNFAIP1 to regulate apoptosis via a SAPK/JNK-mediated signal transduction mechanism.

Original languageEnglish
Pages (from-to)2520-2527
Number of pages8
JournalInternational Journal of Cancer
Volume125
Issue number11
DOIs
Publication statusPublished - 2009 Dec 1

Fingerprint

HeLa Cells
Apoptosis
rhoB GTP-Binding Protein
Two-Hybrid System Techniques
Endosomes
Small Interfering RNA
Signal Transduction
Neoplasms
Phosphorylation
Observation

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Kim, D. M., Chung, K. S., Choi, S. J., Jung, Y. J., Park, S. K., Han, G. H., ... Seo, Y. S. (2009). RhoB induces apoptosis via direct interaction with TNFAIP1 in HeLa cells. International Journal of Cancer, 125(11), 2520-2527. https://doi.org/10.1002/ijc.24617
Kim, Dong Myung ; Chung, Kyung Sook ; Choi, Shin Jung ; Jung, Yu Jin ; Park, Song Kyu ; Han, Gyoon Hee ; Ha, Jae Seok ; Song, Kyung Bin ; Choi, Nam Song ; Kim, Hwan Mook ; Won, Misun ; Seo, Yeon Soo. / RhoB induces apoptosis via direct interaction with TNFAIP1 in HeLa cells. In: International Journal of Cancer. 2009 ; Vol. 125, No. 11. pp. 2520-2527.
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title = "RhoB induces apoptosis via direct interaction with TNFAIP1 in HeLa cells",
abstract = "RhoB, a tumor suppressor, has emerged as an interesting cancer target, and extensive studies aimed at understanding its role in apoptosis have been performed. In our study, we investigated the involvement of RhoB-interacting molecules in apoptosis. To identify RhoB-interacting proteins, we performed yeast-two hybrid screening assays using RhoB as a bait and isolated TNFAIP1, a TNFα-induced protein containing the BTB/POZ domain. The interaction between RhoB and TNFAIP1 was demonstrated in vivo through coimmunoprecipitation studies and in vitro binding assays. RFP-TNFAIP1 was found to be partially colocalized with EGFP-RhoB. The partial colocalization of RhoB and TNFAIP1 in endosomes suggests that RhoB-TNFAIP1 interactions may have a functional role in apoptosis. TNFAIP1 elicited proapoptotic activity, while simultaneous expression of RhoB and TNFAIP1 resulted in a dramatic increase in apoptosis in HeLa cells. Furthermore, knockdown of RhoB using siRNA clearly rescued cells from apoptosis induced by TNFAIP1. This finding suggests that interactions between RhoB and TNFAIP1 are crucial for induction of apoptosis in HeLa cells. The observation of increased SAPK/JNK phosphorylation in apoptotic cells and the finding that a JNK inhibitor suppressed apoptosis indicates that SAPK/JNK signaling may be involved in apoptosis induced by RhoB-TNFAIP1 interactions. In conclusion, we found that RhoB interacts with TNFAIP1 to regulate apoptosis via a SAPK/JNK-mediated signal transduction mechanism.",
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Kim, DM, Chung, KS, Choi, SJ, Jung, YJ, Park, SK, Han, GH, Ha, JS, Song, KB, Choi, NS, Kim, HM, Won, M & Seo, YS 2009, 'RhoB induces apoptosis via direct interaction with TNFAIP1 in HeLa cells', International Journal of Cancer, vol. 125, no. 11, pp. 2520-2527. https://doi.org/10.1002/ijc.24617

RhoB induces apoptosis via direct interaction with TNFAIP1 in HeLa cells. / Kim, Dong Myung; Chung, Kyung Sook; Choi, Shin Jung; Jung, Yu Jin; Park, Song Kyu; Han, Gyoon Hee; Ha, Jae Seok; Song, Kyung Bin; Choi, Nam Song; Kim, Hwan Mook; Won, Misun; Seo, Yeon Soo.

In: International Journal of Cancer, Vol. 125, No. 11, 01.12.2009, p. 2520-2527.

Research output: Contribution to journalArticle

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T1 - RhoB induces apoptosis via direct interaction with TNFAIP1 in HeLa cells

AU - Kim, Dong Myung

AU - Chung, Kyung Sook

AU - Choi, Shin Jung

AU - Jung, Yu Jin

AU - Park, Song Kyu

AU - Han, Gyoon Hee

AU - Ha, Jae Seok

AU - Song, Kyung Bin

AU - Choi, Nam Song

AU - Kim, Hwan Mook

AU - Won, Misun

AU - Seo, Yeon Soo

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