Rhynchosia volubilis Promotes Cell Survival via cAMP-PKA/ERK-CREB Pathway

Sang Hyun Ahn; Jung Soo Suh; Yoon Kwan Jang; Heon Su Kim; Gyu Ho Choi; Eunhye Kim; Tae Jin Kim

doi:10.3390/ph15010073

Rhynchosia volubilis Promotes Cell Survival via cAMP-PKA/ERK-CREB Pathway

Sang Hyun Ahn, Jung Soo Suh, Yoon Kwan Jang, Heon Su Kim, Gyu Ho Choi, Eunhye Kim, Tae Jin Kim

Department of Internal Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Rhynchosia volubilis, a small black bean, has been used as a traditional remedy to treat diseases and maintain health in East Asia, but its cellular effects and molecular mechanisms are not fully understood. The purpose of this study was to investigate the effect of ethanol extract from Rhynchosia volubilis (EERV) on cell survival and to elucidate the biochemical signaling pathways. Our results showed that EERV stimulated the cyclic AMP (cAMP) signal revealed by a fluorescent protein (FP)-based intensiometric sensor. Using a Förster resonance energy transfer (FRET)-based sensor, we further revealed that EERV could activate PKA and ERK signals, which are downstream effectors of cAMP. In addition, we reported that EERV could induce the phosphorylation of CREB, a key signal for cell survival. Thus, our results suggested that EERV protects against apoptosis by activating the cell survival pathway through the cAMP-PKA/ERK-CREB pathway.

Original language	English
Article number	73
Journal	Pharmaceuticals
Volume	15
Issue number	1
DOIs	https://doi.org/10.3390/ph15010073
Publication status	Published - 2022 Jan

Bibliographical note

Funding Information:
Funding: This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (2020R1C1C1010107).

Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.

All Science Journal Classification (ASJC) codes

Molecular Medicine
Pharmaceutical Science
Drug Discovery

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title = "Rhynchosia volubilis Promotes Cell Survival via cAMP-PKA/ERK-CREB Pathway",

abstract = "Rhynchosia volubilis, a small black bean, has been used as a traditional remedy to treat diseases and maintain health in East Asia, but its cellular effects and molecular mechanisms are not fully understood. The purpose of this study was to investigate the effect of ethanol extract from Rhynchosia volubilis (EERV) on cell survival and to elucidate the biochemical signaling pathways. Our results showed that EERV stimulated the cyclic AMP (cAMP) signal revealed by a fluorescent protein (FP)-based intensiometric sensor. Using a F{\"o}rster resonance energy transfer (FRET)-based sensor, we further revealed that EERV could activate PKA and ERK signals, which are downstream effectors of cAMP. In addition, we reported that EERV could induce the phosphorylation of CREB, a key signal for cell survival. Thus, our results suggested that EERV protects against apoptosis by activating the cell survival pathway through the cAMP-PKA/ERK-CREB pathway.",

author = "Ahn, {Sang Hyun} and Suh, {Jung Soo} and Jang, {Yoon Kwan} and Kim, {Heon Su} and Choi, {Gyu Ho} and Eunhye Kim and Kim, {Tae Jin}",

note = "Funding Information: Funding: This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (2020R1C1C1010107). Publisher Copyright: {\textcopyright} 2022 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2022",

month = jan,

doi = "10.3390/ph15010073",

language = "English",

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AU - Ahn, Sang Hyun

AU - Suh, Jung Soo

AU - Jang, Yoon Kwan

AU - Kim, Heon Su

AU - Choi, Gyu Ho

AU - Kim, Eunhye

AU - Kim, Tae Jin

N1 - Funding Information: Funding: This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (2020R1C1C1010107). Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2022/1

Y1 - 2022/1

N2 - Rhynchosia volubilis, a small black bean, has been used as a traditional remedy to treat diseases and maintain health in East Asia, but its cellular effects and molecular mechanisms are not fully understood. The purpose of this study was to investigate the effect of ethanol extract from Rhynchosia volubilis (EERV) on cell survival and to elucidate the biochemical signaling pathways. Our results showed that EERV stimulated the cyclic AMP (cAMP) signal revealed by a fluorescent protein (FP)-based intensiometric sensor. Using a Förster resonance energy transfer (FRET)-based sensor, we further revealed that EERV could activate PKA and ERK signals, which are downstream effectors of cAMP. In addition, we reported that EERV could induce the phosphorylation of CREB, a key signal for cell survival. Thus, our results suggested that EERV protects against apoptosis by activating the cell survival pathway through the cAMP-PKA/ERK-CREB pathway.

AB - Rhynchosia volubilis, a small black bean, has been used as a traditional remedy to treat diseases and maintain health in East Asia, but its cellular effects and molecular mechanisms are not fully understood. The purpose of this study was to investigate the effect of ethanol extract from Rhynchosia volubilis (EERV) on cell survival and to elucidate the biochemical signaling pathways. Our results showed that EERV stimulated the cyclic AMP (cAMP) signal revealed by a fluorescent protein (FP)-based intensiometric sensor. Using a Förster resonance energy transfer (FRET)-based sensor, we further revealed that EERV could activate PKA and ERK signals, which are downstream effectors of cAMP. In addition, we reported that EERV could induce the phosphorylation of CREB, a key signal for cell survival. Thus, our results suggested that EERV protects against apoptosis by activating the cell survival pathway through the cAMP-PKA/ERK-CREB pathway.

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Rhynchosia volubilis Promotes Cell Survival via cAMP-PKA/ERK-CREB Pathway

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