Ribonucleoprotein complexes of hepatitis delta virus

W. S. Ryu, H. J. Netter, M. Bayer, J. Taylor

Research output: Contribution to journalArticle

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Abstract

Human hepatitis delta virus (HDV) is a subviral satellite agent of hepatitis B virus (HBV). The envelope proteins of HDV are provided by the helper virus, HBV, but very little is known about the internal structure of HDV. The particles contain multiple copies of the delta antigen and an unusual RNA genome that is small, about 1,700 nucleotides in length, single stranded, and circular. By using UV cross-linking, equilibrium density centrifugation, and immunoprecipitation, we obtained evidence consistent with the interpretation that delta antigen and genomic RNA form a stable ribonucleoprotein (RNP) complex within the virion. Furthermore, electron- microscopic examination of the purified viral RNP revealed a roughly spherical core-like structure with a diameter of 18.7 ± 2.5 nm. We also isolated HDV-specific RNP structures from the nuclei of cells undergoing HDV genome replication; both the genome and antigenome (a complement of the genome) of HDV were found to be in such complexes. From the equilibrium density analyses of the viral and nuclear RNPs, we were able to deduce the number of molecules of delta antigen per molecule of HDV RNA. For virions, this number was predominantly ca. 70, which was larger than for the nuclear RNPs, which were more heterogeneous, with an average value of ca. 30.

Original languageEnglish
Pages (from-to)3281-3287
Number of pages7
JournalJournal of Virology
Volume67
Issue number6
Publication statusPublished - 1993 Jan 1

Fingerprint

Hepatitis delta virus
Hepatitis Delta Virus
ribonucleoproteins
Ribonucleoproteins
Hepatitis delta Antigens
Genome
genome
Hepatitis B virus
RNA
antigens
virion
Virion
Cell Nucleus Structures
Helper Viruses
cell nucleus
Virus Replication
Centrifugation
crosslinking
Immunoprecipitation
centrifugation

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Ryu, W. S., Netter, H. J., Bayer, M., & Taylor, J. (1993). Ribonucleoprotein complexes of hepatitis delta virus. Journal of Virology, 67(6), 3281-3287.
Ryu, W. S. ; Netter, H. J. ; Bayer, M. ; Taylor, J. / Ribonucleoprotein complexes of hepatitis delta virus. In: Journal of Virology. 1993 ; Vol. 67, No. 6. pp. 3281-3287.
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Ryu, WS, Netter, HJ, Bayer, M & Taylor, J 1993, 'Ribonucleoprotein complexes of hepatitis delta virus', Journal of Virology, vol. 67, no. 6, pp. 3281-3287.

Ribonucleoprotein complexes of hepatitis delta virus. / Ryu, W. S.; Netter, H. J.; Bayer, M.; Taylor, J.

In: Journal of Virology, Vol. 67, No. 6, 01.01.1993, p. 3281-3287.

Research output: Contribution to journalArticle

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Ryu WS, Netter HJ, Bayer M, Taylor J. Ribonucleoprotein complexes of hepatitis delta virus. Journal of Virology. 1993 Jan 1;67(6):3281-3287.