Rice bending lamina 2 (bla2) mutants are defective in a Cytochrome P450 (CYP734A6) gene predicted to mediate brassinosteroid catabolism

Wanki Park, Bang Kim Ho, Taek Kim Woo, Bum Park Phun, Gynheung An, Sunghwa Choe

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9 Citations (Scopus)

Abstract

Desirable morphological traits in rice plants, such as shorter stalks and erect leaves, are being pursued in breeding programs. Rice brassinosteroid (BR)-deficient mutants display the phenotype of reduced heights and erect lamina. Therefore, biotechnology can be used for controlled deactivation of bioactive BRs in specific tissues. Here, we isolated a gene encoding for a BR-deactivating enzyme. Based on its sequence identity with a known enzyme, Arabidopsis Cytochrome P450 734A1, we identified a rice homolog named OsCYP734A6 (LOC_Os01g29150). A search of the rice T-DNA mutant population for insertional mutants of this gene revealed two alleles. These T-DNA loss-of-function mutants displayed a strongly bending lamina phenotype similar to the morphology of rice plants treated with BRs. These two mutants were named bending lamina (bla)2-1 and bla2-2. In Arabidopsis, the transcript level of a putative BR biosynthetic gene, OsDWARF4, is feed-back down-regulated in response to exogenous application of BRs. Similarly, a steady-state level of OsDWARF4 transcripts was significantly down-regulated here in the bla2 mutant background. Based on their sequence similarity, the bending-lamina phenotype in the bla2 mutant, and down-regulation of the BR biosynthetic gene OsDWARF4, we therefore propose that OsCYP734A6 (BLA2) is likely to be involved in BR deactivation.

Original languageEnglish
Pages (from-to)469-476
Number of pages8
JournalJournal of Plant Biology
Volume49
Issue number6
DOIs
Publication statusPublished - 2006 Dec 31

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brassinosteroids
laminae (animals)
cytochrome P-450
leaf blade
rice
mutants
metabolism
genes
phenotype
Arabidopsis
DNA
enzymes
biotechnology
alleles
breeding
leaves

All Science Journal Classification (ASJC) codes

  • Plant Science

Cite this

Park, Wanki ; Ho, Bang Kim ; Woo, Taek Kim ; Phun, Bum Park ; An, Gynheung ; Choe, Sunghwa. / Rice bending lamina 2 (bla2) mutants are defective in a Cytochrome P450 (CYP734A6) gene predicted to mediate brassinosteroid catabolism. In: Journal of Plant Biology. 2006 ; Vol. 49, No. 6. pp. 469-476.
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abstract = "Desirable morphological traits in rice plants, such as shorter stalks and erect leaves, are being pursued in breeding programs. Rice brassinosteroid (BR)-deficient mutants display the phenotype of reduced heights and erect lamina. Therefore, biotechnology can be used for controlled deactivation of bioactive BRs in specific tissues. Here, we isolated a gene encoding for a BR-deactivating enzyme. Based on its sequence identity with a known enzyme, Arabidopsis Cytochrome P450 734A1, we identified a rice homolog named OsCYP734A6 (LOC_Os01g29150). A search of the rice T-DNA mutant population for insertional mutants of this gene revealed two alleles. These T-DNA loss-of-function mutants displayed a strongly bending lamina phenotype similar to the morphology of rice plants treated with BRs. These two mutants were named bending lamina (bla)2-1 and bla2-2. In Arabidopsis, the transcript level of a putative BR biosynthetic gene, OsDWARF4, is feed-back down-regulated in response to exogenous application of BRs. Similarly, a steady-state level of OsDWARF4 transcripts was significantly down-regulated here in the bla2 mutant background. Based on their sequence similarity, the bending-lamina phenotype in the bla2 mutant, and down-regulation of the BR biosynthetic gene OsDWARF4, we therefore propose that OsCYP734A6 (BLA2) is likely to be involved in BR deactivation.",
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Rice bending lamina 2 (bla2) mutants are defective in a Cytochrome P450 (CYP734A6) gene predicted to mediate brassinosteroid catabolism. / Park, Wanki; Ho, Bang Kim; Woo, Taek Kim; Phun, Bum Park; An, Gynheung; Choe, Sunghwa.

In: Journal of Plant Biology, Vol. 49, No. 6, 31.12.2006, p. 469-476.

Research output: Contribution to journalArticle

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