RIP: A novel protein containing a death domain that interacts with Fas/APO-1 (CD95) in yeast and causes cell death

Ben Z. Stanger, Philip Leder, Tae Ho Lee, Emily Kim, Brian Seed

Research output: Contribution to journalArticle

788 Citations (Scopus)

Abstract

Ligation of the extracellular domain of the cell surface receptor Fas/APO-1 (CD95) elicits a characteristic programmed death response in susceptible cells. Using a genetic selection based on protein-protein interaction in yeast, we have identified two gene products that associate with the intracellular domain of Fas: Fas itself, and a novel 74 kDa protein we have named RIP, for receptor interacting protein. RIP also interacts weakly with the p55 tumor necrosis factor receptor (TNFRI) intracellular domain, but not with a mutant version of Fas corresponding to the murine Iprocg mutation. RIP contains an N-terminal region with homology to protein kinases and a C-terminal region containing a cytoplasmic motif (death domain) present in the Fas and TNFR1 intracellular domains. Transient over-expression of RIP causes transfected cells to undergo the morphological changes characteristic of apoptosis. Taken together, these properties indicate that RIP is a novel form of apoptosis-inducing protein.

Original languageEnglish
Pages (from-to)513-523
Number of pages11
JournalCell
Volume81
Issue number4
DOIs
Publication statusPublished - 1995 May 19

Fingerprint

Cell death
Yeast
Cause of Death
Cell Death
Yeasts
Receptor-Interacting Protein Serine-Threonine Kinases
Receptors, Tumor Necrosis Factor, Type I
Apoptosis Regulatory Proteins
Proteins
Tumor Necrosis Factor Receptors
Genetic Selection
Cell Surface Receptors
Protein Kinases
Protein Kinase C
Ligation
Genes
Apoptosis
Mutation
Death Domain

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Stanger, Ben Z. ; Leder, Philip ; Lee, Tae Ho ; Kim, Emily ; Seed, Brian. / RIP : A novel protein containing a death domain that interacts with Fas/APO-1 (CD95) in yeast and causes cell death. In: Cell. 1995 ; Vol. 81, No. 4. pp. 513-523.
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RIP : A novel protein containing a death domain that interacts with Fas/APO-1 (CD95) in yeast and causes cell death. / Stanger, Ben Z.; Leder, Philip; Lee, Tae Ho; Kim, Emily; Seed, Brian.

In: Cell, Vol. 81, No. 4, 19.05.1995, p. 513-523.

Research output: Contribution to journalArticle

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