RNA aptamer-based sensitive detection of SARS coronavirus nucleocapsid protein

Dae Gyun Ahn, Il Ji Jeon, Jung Dong Kim, Min Sun Song, Seung Ryul Han, Seong Wook Lee, Hyungil Jung, Jong Won Oh

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Severe acute respiratory syndrome coronavirus (SARS-CoV) is the etiological agent of a newly emerged disease SARS. The SARS-CoV nucleocapsid (N) protein is one of the most abundant structural proteins and serves as a diagnostic marker for accurate and sensitive detection of the virus. Using a SELEX (systematic evolution of ligand by exponential enrichment) procedure and recombinant N protein, we selected a high-affinity RNA aptamer capable of binding to N protein with a dissociation constant of 1.65 nM. Electrophoretic mobility shift assays and RNA competition experiments showed that the selected aptamer recognized selectively the C-terminal region of N protein with high specificity. Using a chemiluminescence immunosorbent assay and a nanoarray aptamer chip with the selected aptamer as an antigen-capturing agent, we could sensitively detect N protein at a concentration as low as 2 pg/ml. These aptamer-antibody hybrid immunoassays may be useful for rapid, sensitive detection of SARS-CoV N protein.

Original languageEnglish
Pages (from-to)1896-1901
Number of pages6
JournalAnalyst
Volume134
Issue number9
DOIs
Publication statusPublished - 2009

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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  • Cite this

    Ahn, D. G., Jeon, I. J., Kim, J. D., Song, M. S., Han, S. R., Lee, S. W., Jung, H., & Oh, J. W. (2009). RNA aptamer-based sensitive detection of SARS coronavirus nucleocapsid protein. Analyst, 134(9), 1896-1901. https://doi.org/10.1039/b906788d