RNA aptamer-based sensitive detection of SARS coronavirus nucleocapsid protein

Dae Gyun Ahn, Il Ji Jeon, Jung Dong Kim, Min Sun Song, Seung Ryul Han, Seong Wook Lee, Hyungil Jung, Jong-Won Oh

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Severe acute respiratory syndrome coronavirus (SARS-CoV) is the etiological agent of a newly emerged disease SARS. The SARS-CoV nucleocapsid (N) protein is one of the most abundant structural proteins and serves as a diagnostic marker for accurate and sensitive detection of the virus. Using a SELEX (systematic evolution of ligand by exponential enrichment) procedure and recombinant N protein, we selected a high-affinity RNA aptamer capable of binding to N protein with a dissociation constant of 1.65 nM. Electrophoretic mobility shift assays and RNA competition experiments showed that the selected aptamer recognized selectively the C-terminal region of N protein with high specificity. Using a chemiluminescence immunosorbent assay and a nanoarray aptamer chip with the selected aptamer as an antigen-capturing agent, we could sensitively detect N protein at a concentration as low as 2 pg/ml. These aptamer-antibody hybrid immunoassays may be useful for rapid, sensitive detection of SARS-CoV N protein.

Original languageEnglish
Pages (from-to)1896-1901
Number of pages6
JournalAnalyst
Volume134
Issue number9
DOIs
Publication statusPublished - 2009 Sep 28

Fingerprint

SARS Virus
Nucleotide Aptamers
severe acute respiratory syndrome
RNA
Severe Acute Respiratory Syndrome
Proteins
protein
SELEX Aptamer Technique
Immunosorbents
Coronavirus
Assays
Electrophoretic Mobility Shift Assay
Luminescence
Immunoassay
Recombinant Proteins
Nucleocapsid
Electrophoretic mobility
assay
Chemiluminescence
Viruses

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Spectroscopy
  • Electrochemistry
  • Biochemistry
  • Environmental Chemistry

Cite this

Ahn, D. G., Jeon, I. J., Kim, J. D., Song, M. S., Han, S. R., Lee, S. W., ... Oh, J-W. (2009). RNA aptamer-based sensitive detection of SARS coronavirus nucleocapsid protein. Analyst, 134(9), 1896-1901. https://doi.org/10.1039/b906788d
Ahn, Dae Gyun ; Jeon, Il Ji ; Kim, Jung Dong ; Song, Min Sun ; Han, Seung Ryul ; Lee, Seong Wook ; Jung, Hyungil ; Oh, Jong-Won. / RNA aptamer-based sensitive detection of SARS coronavirus nucleocapsid protein. In: Analyst. 2009 ; Vol. 134, No. 9. pp. 1896-1901.
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Ahn, DG, Jeon, IJ, Kim, JD, Song, MS, Han, SR, Lee, SW, Jung, H & Oh, J-W 2009, 'RNA aptamer-based sensitive detection of SARS coronavirus nucleocapsid protein', Analyst, vol. 134, no. 9, pp. 1896-1901. https://doi.org/10.1039/b906788d

RNA aptamer-based sensitive detection of SARS coronavirus nucleocapsid protein. / Ahn, Dae Gyun; Jeon, Il Ji; Kim, Jung Dong; Song, Min Sun; Han, Seung Ryul; Lee, Seong Wook; Jung, Hyungil; Oh, Jong-Won.

In: Analyst, Vol. 134, No. 9, 28.09.2009, p. 1896-1901.

Research output: Contribution to journalArticle

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Ahn DG, Jeon IJ, Kim JD, Song MS, Han SR, Lee SW et al. RNA aptamer-based sensitive detection of SARS coronavirus nucleocapsid protein. Analyst. 2009 Sep 28;134(9):1896-1901. https://doi.org/10.1039/b906788d