RNA-binding as chaperones of DNA binding proteins from starved cells

Chan Park, Yoontae Jin, Young Jun Kim, Hotcherl Jeong, Baik L. Seong

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

DNA-binding proteins from starved cells (Dps) in Escherichia coli protects DNA from multiple stresses during the stationary phase by forming a stable Dps-DNA complex. In contrast, Dps cannot bind to DNA during the exponential phase and it has not been clear why Dps conditionally binds to DNA depending on the growth phase. In this study, we show that DNA-free Dps in the exponential phase can also bind to RNA and the preemptive binding of RNA precludes DNA from interacting with Dps. The critical role of RNA in modulating the stability and functional competence of Dps and their morphology, leads us to propose a two-state model of Dps in executing stress responses. In the exponential phase, Dps is present predominantly as ribonucleoprotein complex. Under starvation, RNAs are degraded by up-regulated RNases, activating Dps to bind with chromosomal DNAs protecting them from diverse stresses. A dual role of RNA as an inhibitor of DNA binding and chaperone to keep dynamic functional status of Dps would be crucial for operating an immediate protection of chromosomal DNAs on starvation. The holdase-type chaperoning role of RNA in Dps-mediated stress responses would shed light on the role of RNAs as chaperone (Chaperna).

Original languageEnglish
Pages (from-to)484-489
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume524
Issue number2
DOIs
Publication statusPublished - 2020 Apr 2

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'RNA-binding as chaperones of DNA binding proteins from starved cells'. Together they form a unique fingerprint.

  • Cite this