RNA-dependent assembly of chimeric antigen nanoparticles as an efficient H5N1 pre-pandemic vaccine platform

Jongkwan Lim; Yucheol Cheong; Young Seok Kim; Wonil Chae; Beom Jeung Hwang; Jinhee Lee; Yo Han Jang; Young Hoon Roh; Sang Uk Seo; Baik L. Seong

doi:10.1016/j.nano.2021.102438

RNA-dependent assembly of chimeric antigen nanoparticles as an efficient H5N1 pre-pandemic vaccine platform

Jongkwan Lim, Yucheol Cheong, Young Seok Kim, Wonil Chae, Beom Jeung Hwang, Jinhee Lee, Yo Han Jang, Young Hoon Roh, Sang Uk Seo, Baik L. Seong

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Highly pathogenic avian influenza viruses (HPAIVs) pose a significant threat to human health, with high mortality rates, and require effective vaccines. We showed that, harnessed with novel RNA-mediated chaperone function, hemagglutinin (HA) of H5N1 HPAIV could be displayed as an immunologically relevant conformation on self-assembled chimeric nanoparticles (cNP). A tri-partite monomeric antigen was designed including: i) an RNA-interaction domain (RID) as a docking tag for RNA to enable chaperna function (chaperna: chaperone + RNA), ii) globular head domain (gd) of HA as a target antigen, and iii) ferritin as a scaffold for 24 mer-assembly. The immunization of mice with the nanoparticles (~46 nm) induced a 25–30 fold higher neutralizing capacity of the antibody and provided cross-protection from homologous and heterologous lethal challenges. This study suggests that cNP assembly is conducive to eliciting antibodies against the conserved region in HA, providing potent and broad protective efficacy.

Original language	English
Article number	102438
Journal	Nanomedicine: Nanotechnology, Biology, and Medicine
Volume	37
DOIs	https://doi.org/10.1016/j.nano.2021.102438
Publication status	Published - 2021 Oct

Bibliographical note

Funding Information:
This work was supported by the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), the Ministry of Health & Welfare, Republic of Korea [grant number HV20C0070 ]; the National Research Foundation of Korea [grant number 2018M3A9H4079358 ]; Brain Korea 21 (BK21) FOUR program .

Funding Information:
Support: This work was supported by the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), the Ministry of Health & Welfare, Republic of Korea [grant number HV20C0070]; the National Research Foundation of Korea [grant number 2018M3A9H4079358]; Brain Korea 21 (BK21) FOUR program.

Publisher Copyright:
© 2021 The Authors

All Science Journal Classification (ASJC) codes

Bioengineering
Medicine (miscellaneous)
Molecular Medicine
Biomedical Engineering
Materials Science(all)
Pharmaceutical Science

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.nano.2021.102438

Cite this

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title = "RNA-dependent assembly of chimeric antigen nanoparticles as an efficient H5N1 pre-pandemic vaccine platform",

abstract = "Highly pathogenic avian influenza viruses (HPAIVs) pose a significant threat to human health, with high mortality rates, and require effective vaccines. We showed that, harnessed with novel RNA-mediated chaperone function, hemagglutinin (HA) of H5N1 HPAIV could be displayed as an immunologically relevant conformation on self-assembled chimeric nanoparticles (cNP). A tri-partite monomeric antigen was designed including: i) an RNA-interaction domain (RID) as a docking tag for RNA to enable chaperna function (chaperna: chaperone + RNA), ii) globular head domain (gd) of HA as a target antigen, and iii) ferritin as a scaffold for 24 mer-assembly. The immunization of mice with the nanoparticles (~46 nm) induced a 25–30 fold higher neutralizing capacity of the antibody and provided cross-protection from homologous and heterologous lethal challenges. This study suggests that cNP assembly is conducive to eliciting antibodies against the conserved region in HA, providing potent and broad protective efficacy.",

author = "Jongkwan Lim and Yucheol Cheong and Kim, {Young Seok} and Wonil Chae and Hwang, {Beom Jeung} and Jinhee Lee and Jang, {Yo Han} and Roh, {Young Hoon} and Seo, {Sang Uk} and Seong, {Baik L.}",

note = "Funding Information: This work was supported by the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), the Ministry of Health & Welfare, Republic of Korea [grant number HV20C0070 ]; the National Research Foundation of Korea [grant number 2018M3A9H4079358 ]; Brain Korea 21 (BK21) FOUR program . Funding Information: Support: This work was supported by the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), the Ministry of Health & Welfare, Republic of Korea [grant number HV20C0070]; the National Research Foundation of Korea [grant number 2018M3A9H4079358]; Brain Korea 21 (BK21) FOUR program. Publisher Copyright: {\textcopyright} 2021 The Authors",

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AU - Lim, Jongkwan

AU - Cheong, Yucheol

AU - Kim, Young Seok

AU - Chae, Wonil

AU - Hwang, Beom Jeung

AU - Lee, Jinhee

AU - Jang, Yo Han

AU - Roh, Young Hoon

AU - Seo, Sang Uk

AU - Seong, Baik L.

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N2 - Highly pathogenic avian influenza viruses (HPAIVs) pose a significant threat to human health, with high mortality rates, and require effective vaccines. We showed that, harnessed with novel RNA-mediated chaperone function, hemagglutinin (HA) of H5N1 HPAIV could be displayed as an immunologically relevant conformation on self-assembled chimeric nanoparticles (cNP). A tri-partite monomeric antigen was designed including: i) an RNA-interaction domain (RID) as a docking tag for RNA to enable chaperna function (chaperna: chaperone + RNA), ii) globular head domain (gd) of HA as a target antigen, and iii) ferritin as a scaffold for 24 mer-assembly. The immunization of mice with the nanoparticles (~46 nm) induced a 25–30 fold higher neutralizing capacity of the antibody and provided cross-protection from homologous and heterologous lethal challenges. This study suggests that cNP assembly is conducive to eliciting antibodies against the conserved region in HA, providing potent and broad protective efficacy.

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RNA-dependent assembly of chimeric antigen nanoparticles as an efficient H5N1 pre-pandemic vaccine platform

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

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