Soluble fractions prepared from cells of Pseudomonas carboxydovorans bearing a small plasmid (1.76 × 106) exhibited proteolytic activity on the β-subunit of CO dehydrogenase (CO-DH) in plasmid-cured cells of the same strain, implying that the plasmid carries gene(s) for processing the β subunit of the enzyme at the post-translational level. The protease was found to be a constitutive enzyme. It did not hydrolyze the β subunit of CO-DH in Pseudomonas carboxydohydrogena. Analysis of CO-DH after transformation of the cured cells with the small plasmid confirmed that the plasmid plays a role in the modification of the β subunit of CO-DH in P. carboxydovorans.
|Number of pages||4|
|Journal||FEMS Microbiology Letters|
|Publication status||Published - 1986 Oct|
Bibliographical noteFunding Information:
This work was supported by a grant from the Korea Research Foundation (1985-1986).
All Science Journal Classification (ASJC) codes
- Molecular Biology