Role of NADPH oxidase and calcium in cerulein-induced apoptosis: Involvement of apoptosis-inducing factor

Hoon Yu Ji, Hwan Kim Kyung, Hyeyoung Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

26 Citations (Scopus)

Abstract

Apoptosis linked to oxidative stress has been implicated in pancreatitis. NADPH oxidase has been considered as a major source of reactive oxygen species (ROS) during inflammation and apoptosis in pancreatic acinar cells. Recently we demonstrated that NADPH oxidase subunits Nox1, p27phox, p47 phox, and p67phox are constitutively expressed in pancreatic acinar cells and may contribute to apoptosis in pancreatic acinar AR42J cells stimulated with cerulein. The present study aims to investigate the apoptotic mechanism of pancreatic acinar cells stimulated with cerulein by determining whether cerulein induces apoptosis-inducing factor (AIF) expression and whether cerulein-induced expression of AIF is inhibited by transfection with antisense oligonucleotide (AS ODN) of p47phox or p67phox or treatment with a Ca2+ chelator BAPTA-AM. As a result, cerulein induced the expression of apoptotic gene AIF. Transfection with AS ODN of p47phox or p67phox or treatment with BAPTA-AM inhibited cerulein-induced AIF expression in pancreatic acinar AR42J cells. These results demonstrate that NADPH oxidase and calcium have a role in cerulein-induced apoptosis in pancreatic acinar AR42J cells by inducing the expression of AIF. In conclusion, the increase in intracellular Ca2+ and NADPH oxidase activity may be the upstream event of apoptotic gene (AIF) expression, which contributes to cerulein-induced apoptosis in pancreatic acinar AR42 cells.

Original languageEnglish
Title of host publicationSignal Transduction Pathways, Part A
Subtitle of host publicationApoptotic and Extracellular Signalling
PublisherBlackwell Publishing Inc.
Pages292-297
Number of pages6
ISBN (Print)1573316458, 9781573316453
DOIs
Publication statusPublished - 2006 Dec

Publication series

NameAnnals of the New York Academy of Sciences
Volume1090
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

Fingerprint Dive into the research topics of 'Role of NADPH oxidase and calcium in cerulein-induced apoptosis: Involvement of apoptosis-inducing factor'. Together they form a unique fingerprint.

  • Cite this

    Ji, H. Y., Kyung, H. K., & Kim, H. (2006). Role of NADPH oxidase and calcium in cerulein-induced apoptosis: Involvement of apoptosis-inducing factor. In Signal Transduction Pathways, Part A: Apoptotic and Extracellular Signalling (pp. 292-297). (Annals of the New York Academy of Sciences; Vol. 1090). Blackwell Publishing Inc.. https://doi.org/10.1196/annals.1378.031