Role of nitric oxide and mucus in ischemia/reperfusion-induced gastric mucosal injury in rats

Hyeyoung Kim, Kyung Hwan Kim

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The present study aims at investigating the role of nitric oxide (NO) on the oxidative damage in gastric mucosa of rats which received ischemia/reperfusion (I/R) and its relation to mucus. NO synthesis modulators such as L-arginine and NG-nitro-L-arginine methyl ester (L-NAME) were injected intraperitoneally to the rats 30 min prior to I/R which was induced by clamping the celiac artery and the superior mesenteric artery for 30 min and reperfusion for 1 h. As a result, I/R increased lipid peroxide production and decreased the contents of glutathione (GSH), cGMP and mucus as well as GSH peroxidase activities of gastric mucosa. I/R decreased the activity and protein of NO synthase (NOS) in gastric mucosa. Pretreatment of L-arginine, a substrate for NOS, prevented I/R-induced alterations of gastric mucosa. However, L-NAME, an NOS inhibitor, deteriorated oxidative damage induced by I/R. In conclusion, NO has an antioxidant defensive role on gastric mucosa by maintaining mucus, GSH and GSH peroxidase, which were related to preservation of cGMP and NOS in gastric mucosa.

Original languageEnglish
Pages (from-to)200-207
Number of pages8
JournalPharmacology
Volume62
Issue number4
DOIs
Publication statusPublished - 2001 May 29

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this