Role of p53 in antioxidant defense of HPV-positive cervical carcinoma cells following H2O2 exposure

Boxiao Ding, Sung Gil Chi, Se Heon Kim, Suki Kang, Jae Ho Cho, Dong Su Kim, Nam Hoon Cho

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

In HPV-positive cervical carcinoma cells, p53 protein is functionally antagonized by the E6 oncoprotein. We investigated a possible role of p53 in antioxidant defense of HPV-positive cervical cancer cell lines. We found that SiHa cells containing integrated HPV 16 had higher expression of p53 and exhibited the greatest resistant to H2O2-induced oxidative damage, compared with HeLa, CaSki and ME180 cell lines. Downregulation of p53 resulted in the inhibition of p53-regulated antioxidant enzymes and elevated intracellular ROS in SiHa cells. By contrast, the ROS level was not affected in HeLa, CaSki and ME180 cell lines after inhibition of the p53 protein. Under mild or severe H2O2-induced stress, p53-deficient SiHa cells exhibited much higher ROS levels than control SiHa cells. Furthermore, we analyzed cell viability and apoptosis after H2O2 treatment and found that p53 deficiency sensitized SiHa cells to H2O2 damage. Inhibition of p53 resulted in excessive oxidation of DNA; control SiHa cells exhibited a more rapid removal of 8-oxo-7,8-dihydro-2′-deoxyguanosine from DNA compared with p53-deficient SiHa cells exposed to the same level of H2O2 challenge. These data collectively show that endogenous p53 in SiHa cells has an antioxidant function and involves in the reinforcement of the antioxidant defense.

Original languageEnglish
Pages (from-to)2284-2294
Number of pages11
JournalJournal of cell science
Volume120
Issue number13
DOIs
Publication statusPublished - 2007 Jul 1

Fingerprint

Antioxidants
Carcinoma
Cell Line
Human papillomavirus 16
Oncogene Proteins
DNA
Uterine Cervical Neoplasms
Cell Survival
Proteins
Down-Regulation
Apoptosis
Enzymes

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Ding, Boxiao ; Chi, Sung Gil ; Kim, Se Heon ; Kang, Suki ; Cho, Jae Ho ; Kim, Dong Su ; Cho, Nam Hoon. / Role of p53 in antioxidant defense of HPV-positive cervical carcinoma cells following H2O2 exposure. In: Journal of cell science. 2007 ; Vol. 120, No. 13. pp. 2284-2294.
@article{60c03067ab4f41db87ac877763c7c146,
title = "Role of p53 in antioxidant defense of HPV-positive cervical carcinoma cells following H2O2 exposure",
abstract = "In HPV-positive cervical carcinoma cells, p53 protein is functionally antagonized by the E6 oncoprotein. We investigated a possible role of p53 in antioxidant defense of HPV-positive cervical cancer cell lines. We found that SiHa cells containing integrated HPV 16 had higher expression of p53 and exhibited the greatest resistant to H2O2-induced oxidative damage, compared with HeLa, CaSki and ME180 cell lines. Downregulation of p53 resulted in the inhibition of p53-regulated antioxidant enzymes and elevated intracellular ROS in SiHa cells. By contrast, the ROS level was not affected in HeLa, CaSki and ME180 cell lines after inhibition of the p53 protein. Under mild or severe H2O2-induced stress, p53-deficient SiHa cells exhibited much higher ROS levels than control SiHa cells. Furthermore, we analyzed cell viability and apoptosis after H2O2 treatment and found that p53 deficiency sensitized SiHa cells to H2O2 damage. Inhibition of p53 resulted in excessive oxidation of DNA; control SiHa cells exhibited a more rapid removal of 8-oxo-7,8-dihydro-2′-deoxyguanosine from DNA compared with p53-deficient SiHa cells exposed to the same level of H2O2 challenge. These data collectively show that endogenous p53 in SiHa cells has an antioxidant function and involves in the reinforcement of the antioxidant defense.",
author = "Boxiao Ding and Chi, {Sung Gil} and Kim, {Se Heon} and Suki Kang and Cho, {Jae Ho} and Kim, {Dong Su} and Cho, {Nam Hoon}",
year = "2007",
month = "7",
day = "1",
doi = "10.1242/jcs.002345",
language = "English",
volume = "120",
pages = "2284--2294",
journal = "Journal of Cell Science",
issn = "0021-9533",
publisher = "Company of Biologists Ltd",
number = "13",

}

Role of p53 in antioxidant defense of HPV-positive cervical carcinoma cells following H2O2 exposure. / Ding, Boxiao; Chi, Sung Gil; Kim, Se Heon; Kang, Suki; Cho, Jae Ho; Kim, Dong Su; Cho, Nam Hoon.

In: Journal of cell science, Vol. 120, No. 13, 01.07.2007, p. 2284-2294.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Role of p53 in antioxidant defense of HPV-positive cervical carcinoma cells following H2O2 exposure

AU - Ding, Boxiao

AU - Chi, Sung Gil

AU - Kim, Se Heon

AU - Kang, Suki

AU - Cho, Jae Ho

AU - Kim, Dong Su

AU - Cho, Nam Hoon

PY - 2007/7/1

Y1 - 2007/7/1

N2 - In HPV-positive cervical carcinoma cells, p53 protein is functionally antagonized by the E6 oncoprotein. We investigated a possible role of p53 in antioxidant defense of HPV-positive cervical cancer cell lines. We found that SiHa cells containing integrated HPV 16 had higher expression of p53 and exhibited the greatest resistant to H2O2-induced oxidative damage, compared with HeLa, CaSki and ME180 cell lines. Downregulation of p53 resulted in the inhibition of p53-regulated antioxidant enzymes and elevated intracellular ROS in SiHa cells. By contrast, the ROS level was not affected in HeLa, CaSki and ME180 cell lines after inhibition of the p53 protein. Under mild or severe H2O2-induced stress, p53-deficient SiHa cells exhibited much higher ROS levels than control SiHa cells. Furthermore, we analyzed cell viability and apoptosis after H2O2 treatment and found that p53 deficiency sensitized SiHa cells to H2O2 damage. Inhibition of p53 resulted in excessive oxidation of DNA; control SiHa cells exhibited a more rapid removal of 8-oxo-7,8-dihydro-2′-deoxyguanosine from DNA compared with p53-deficient SiHa cells exposed to the same level of H2O2 challenge. These data collectively show that endogenous p53 in SiHa cells has an antioxidant function and involves in the reinforcement of the antioxidant defense.

AB - In HPV-positive cervical carcinoma cells, p53 protein is functionally antagonized by the E6 oncoprotein. We investigated a possible role of p53 in antioxidant defense of HPV-positive cervical cancer cell lines. We found that SiHa cells containing integrated HPV 16 had higher expression of p53 and exhibited the greatest resistant to H2O2-induced oxidative damage, compared with HeLa, CaSki and ME180 cell lines. Downregulation of p53 resulted in the inhibition of p53-regulated antioxidant enzymes and elevated intracellular ROS in SiHa cells. By contrast, the ROS level was not affected in HeLa, CaSki and ME180 cell lines after inhibition of the p53 protein. Under mild or severe H2O2-induced stress, p53-deficient SiHa cells exhibited much higher ROS levels than control SiHa cells. Furthermore, we analyzed cell viability and apoptosis after H2O2 treatment and found that p53 deficiency sensitized SiHa cells to H2O2 damage. Inhibition of p53 resulted in excessive oxidation of DNA; control SiHa cells exhibited a more rapid removal of 8-oxo-7,8-dihydro-2′-deoxyguanosine from DNA compared with p53-deficient SiHa cells exposed to the same level of H2O2 challenge. These data collectively show that endogenous p53 in SiHa cells has an antioxidant function and involves in the reinforcement of the antioxidant defense.

UR - http://www.scopus.com/inward/record.url?scp=34447521989&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34447521989&partnerID=8YFLogxK

U2 - 10.1242/jcs.002345

DO - 10.1242/jcs.002345

M3 - Article

C2 - 17567683

AN - SCOPUS:34447521989

VL - 120

SP - 2284

EP - 2294

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

IS - 13

ER -