Role of regulators of G-protein signaling 4 in Ca 2+ signaling in mouse pancreatic acinar cells

Soonhong Park, Syng Ill Lee, Dong Min Shin

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Regulators of G-protein signaling (RGS) proteins are regulators of Ca 2+ signaling that accelerate the GTPase activity of the G-protein α-subunit. RGS1, RGS2, RGS4, and RGS16 are expressed in the pancreas, and RGS2 regulates G-protein coupled receptor (GPCR)-induced Ca 2+ oscillations. However, the role of RGS4 in Ca 2+ signaling in pancreatic acinar cells is unknown. In this study, we investigated the mechanism of GPCR-induced Ca 2+ signaling in pancreatic acinar cells derived from RGS4 -/- mice. RGS4 -/- acinar cells showed an enhanced stimulus intensity response to a muscarinic receptor agonist in pancreatic acinar cells. Moreover, deletion of RGS4 increased the frequency of Ca 2+ oscillations. RGS4 -/- cells also showed increased expression of sarco/endoplasmic reticulum Ca 2+ ATPase type 2. However, there were no significant alterations, such as Ca 2+ signaling in treated high dose of agonist and its related amylase secretion activity, in acinar cells from RGS4 -/- mice. These results indicate that RGS4 protein regulates Ca 2+ signaling in mouse pancreatic acinar cells.

Original languageEnglish
Pages (from-to)383-388
Number of pages6
JournalKorean Journal of Physiology and Pharmacology
Volume15
Issue number6
DOIs
Publication statusPublished - 2011 Dec 1

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All Science Journal Classification (ASJC) codes

  • Physiology
  • Pharmacology

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