Role of the purine repressor hinge sequence in repressor function

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

A protease-hypersensitive hinge sequence in Escherichia coli purine repressor (PurR) connects an N-terminal DNA-binding domain with a contiguous corepressor-binding domain. Binding of one molecule of dimeric repressor to operator DNA protects the hinge against proteolytic cleavage. Mutations in the hinge region impair repressor function in vivo. Several nonfunctional hinge mutants were defective in low-affinity binding to operator DNA in the absence of corepressor as well as in high-affinity corepressor-dependent binding to operator DNA, although binding of corepressor was similar to binding of the wild-type repressor. These results establish a role for the hinge region in operator binding and lead to a proposal for two routes to form the holoPurR-operator complex.

Original languageEnglish
Pages (from-to)1767-1772
Number of pages6
JournalJournal of Bacteriology
Volume176
Issue number6
Publication statusPublished - 1994 Jan 1

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Co-Repressor Proteins
DNA
Repressor Proteins
Peptide Hydrolases
Escherichia coli
Mutation
purine

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

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abstract = "A protease-hypersensitive hinge sequence in Escherichia coli purine repressor (PurR) connects an N-terminal DNA-binding domain with a contiguous corepressor-binding domain. Binding of one molecule of dimeric repressor to operator DNA protects the hinge against proteolytic cleavage. Mutations in the hinge region impair repressor function in vivo. Several nonfunctional hinge mutants were defective in low-affinity binding to operator DNA in the absence of corepressor as well as in high-affinity corepressor-dependent binding to operator DNA, although binding of corepressor was similar to binding of the wild-type repressor. These results establish a role for the hinge region in operator binding and lead to a proposal for two routes to form the holoPurR-operator complex.",
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Role of the purine repressor hinge sequence in repressor function. / Kang Yell Choi, Yell Choi; Zalkin, H.

In: Journal of Bacteriology, Vol. 176, No. 6, 01.01.1994, p. 1767-1772.

Research output: Contribution to journalArticle

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