Role of Vascular Endothelial Growth Factor-D (VEGF-D) on IL-6 expression in cerulein-stimulated pancreatic acinar cells

Jangwon Lee, Hwan Kim Kyung, Hyeyoung Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

Cerulein pancreatitis is similar to human edematous pancreatitis with dysregulation of the digestive enzyme production and cytoplasmic vacuolization, the death of acinar cells, edema formation, and an infiltration of inflammatory cells into the pancreas. Our preliminary proteomic analysis showed increased expression of vascular endothelial growth factor-D (VEGF-D) in cerulein (10 -8M)-treated pancreatic acinar cells. We hypothesized that VEGF-D may have a role in cerulein-induced cytokine expression in pancreatic acinar cells. This article was designed to elucidate whether cerulein induces VEGF-D expression in pancreatic AR42J cells, and whether VEGF-D expression is related to interleukin-6 (IL-6) expression by transfecting antisense oligonucleotide (AS ODN) for VEGF-D into pancreatic acinar AR42J cells. Cerulein-induced AP-1 activation was monitored in the cells transfected with AS ODN or sense ODN (S ODN) for VEGF-D. mRNA expression of IL-6 was determined by RT-PCR analysis. AP-1 activation was assessed by electrophoretic mobility shift assay (EMSA). VEGF-D expressionwas assessed by Western blotting. Cerulein induced IL-6 expression and VEGFD expression in AR42J cells. Cerulein-induced AP-1 activation and IL-6 expressionwas inhibited in the cells transfected with ASODN for VEGFD as compared to S ODN. Cerulein-induced VEGF-D expression may have a role in AP-1 activation and IL-6 expression in pancreatic acinar cells.

Original languageEnglish
Title of host publicationSignal Transduction Pathways, Part C
Subtitle of host publicationCell Signaling in Health and Disease
PublisherBlackwell Publishing Inc.
Pages129-133
Number of pages5
ISBN (Print)1573316954, 9781573316958
DOIs
Publication statusPublished - 2007 Jan

Publication series

NameAnnals of the New York Academy of Sciences
Volume1095
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

Fingerprint Dive into the research topics of 'Role of Vascular Endothelial Growth Factor-D (VEGF-D) on IL-6 expression in cerulein-stimulated pancreatic acinar cells'. Together they form a unique fingerprint.

  • Cite this

    Lee, J., Kyung, H. K., & Kim, H. (2007). Role of Vascular Endothelial Growth Factor-D (VEGF-D) on IL-6 expression in cerulein-stimulated pancreatic acinar cells. In Signal Transduction Pathways, Part C: Cell Signaling in Health and Disease (pp. 129-133). (Annals of the New York Academy of Sciences; Vol. 1095). Blackwell Publishing Inc.. https://doi.org/10.1196/annals.1397.016