Abstract
Telomeres are protective nucleoprotein structures at the ends of linear eukaryotic chromosomes. In contrast to double-stranded-specific telomerebinding proteins, the cellular roles of single-stranded-specific telomeric proteins are not well understood in higher plants. Three highly conserved tobacco G-strand-specific telomere-binding protein paralogs (NtGTBP1, NtGTBP2 and NtGTBP3) were identified and characterized. All three NtGTBPs were able to bind specifically to the plant singlestranded telomeric repeat elements in vitro with similar affinities. Suppression of NtGTBP1 by means of the RNAimediated gene knock-down method resulted in developmental defects in transgenic tobacco plants accompanied by lengthened telomeres, extra-chromosomal telomeric circles and abnormal anaphase bridges. These results suggest that the downregulation of NtGTBP1 results in genome instability. NtGTBP1 prevented in vitro strand invasion, a prerequisite process for inter-chromosomal telomeric recombination. Therefore, tobacco NtGTBP1 is one of the essential factors for telomere stability. Because abnormal telomeric elongation and recombination due to the suppression of NtGTBP1 are reminiscent of the recombinational telomere lengthening mechanism that purportedly operates in telomerase negative cancer cells, it is of interest to investigate whether telomeric recombination is associated with cell death in animal systems.
| Original language | English |
|---|---|
| Pages (from-to) | 523-525 |
| Number of pages | 3 |
| Journal | Plant Signaling and Behavior |
| Volume | 6 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2011 Apr 1 |
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All Science Journal Classification (ASJC) codes
- Plant Science
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Roles of NtGTBP1 in telomere stability. / Lee, Yong Woo; Kim, Woo Taek.
In: Plant Signaling and Behavior, Vol. 6, No. 4, 01.04.2011, p. 523-525.Research output: Contribution to journal › Article
TY - JOUR
T1 - Roles of NtGTBP1 in telomere stability
AU - Lee, Yong Woo
AU - Kim, Woo Taek
PY - 2011/4/1
Y1 - 2011/4/1
N2 - Telomeres are protective nucleoprotein structures at the ends of linear eukaryotic chromosomes. In contrast to double-stranded-specific telomerebinding proteins, the cellular roles of single-stranded-specific telomeric proteins are not well understood in higher plants. Three highly conserved tobacco G-strand-specific telomere-binding protein paralogs (NtGTBP1, NtGTBP2 and NtGTBP3) were identified and characterized. All three NtGTBPs were able to bind specifically to the plant singlestranded telomeric repeat elements in vitro with similar affinities. Suppression of NtGTBP1 by means of the RNAimediated gene knock-down method resulted in developmental defects in transgenic tobacco plants accompanied by lengthened telomeres, extra-chromosomal telomeric circles and abnormal anaphase bridges. These results suggest that the downregulation of NtGTBP1 results in genome instability. NtGTBP1 prevented in vitro strand invasion, a prerequisite process for inter-chromosomal telomeric recombination. Therefore, tobacco NtGTBP1 is one of the essential factors for telomere stability. Because abnormal telomeric elongation and recombination due to the suppression of NtGTBP1 are reminiscent of the recombinational telomere lengthening mechanism that purportedly operates in telomerase negative cancer cells, it is of interest to investigate whether telomeric recombination is associated with cell death in animal systems.
AB - Telomeres are protective nucleoprotein structures at the ends of linear eukaryotic chromosomes. In contrast to double-stranded-specific telomerebinding proteins, the cellular roles of single-stranded-specific telomeric proteins are not well understood in higher plants. Three highly conserved tobacco G-strand-specific telomere-binding protein paralogs (NtGTBP1, NtGTBP2 and NtGTBP3) were identified and characterized. All three NtGTBPs were able to bind specifically to the plant singlestranded telomeric repeat elements in vitro with similar affinities. Suppression of NtGTBP1 by means of the RNAimediated gene knock-down method resulted in developmental defects in transgenic tobacco plants accompanied by lengthened telomeres, extra-chromosomal telomeric circles and abnormal anaphase bridges. These results suggest that the downregulation of NtGTBP1 results in genome instability. NtGTBP1 prevented in vitro strand invasion, a prerequisite process for inter-chromosomal telomeric recombination. Therefore, tobacco NtGTBP1 is one of the essential factors for telomere stability. Because abnormal telomeric elongation and recombination due to the suppression of NtGTBP1 are reminiscent of the recombinational telomere lengthening mechanism that purportedly operates in telomerase negative cancer cells, it is of interest to investigate whether telomeric recombination is associated with cell death in animal systems.
UR - http://www.scopus.com/inward/record.url?scp=79955608518&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79955608518&partnerID=8YFLogxK
U2 - 10.4161/psb.6.4.14749
DO - 10.4161/psb.6.4.14749
M3 - Article
C2 - 21474994
AN - SCOPUS:79955608518
VL - 6
SP - 523
EP - 525
JO - Plant Signaling and Behavior
JF - Plant Signaling and Behavior
SN - 1559-2316
IS - 4
ER -