ROS-dependent HMGB1 secretion upregulates IL-8 in upper airway epithelial cells under hypoxic condition

H. J. Min, J. H. Kim, J. E. Yoo, J. H. Oh, K. S. Kim, J. H. Yoon, Chang-Hoon Kim

Research output: Contribution to journalArticle

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Abstract

High-mobility group box 1 (HMGB1) mediates various functions according to the location. We tried to investigate the role of HMGB1 in upper airway under hypoxic conditions. We cultured primary normal human nasal epithelium (NHNE) cells under hypoxic conditions and evaluated the movement of HMGB1 by western blotting, immunofluorescence, and enzyme-linked immunosorbent assay (ELISA). Reactive oxygen species (ROS) level was evaluated to estimate the translocation mechanism of HMGB1. The role of secreted HMGB1 was evaluated by ELISA assay. Furthermore, we collected human nasal mucosa samples and nasal lavage fluids from patients conditioned under hypoxic and non-hypoxic environment, and compared the expression of HMGB1 in human nasal mucosa samples by immunohistochemistry and the levels of HMGB1 in lavage fluids using ELISA assay. Hypoxia induced translocation of HMGB1 into the extracellular area and it was dependent on ROS produced by dual oxidase 2. Secreted HMGB1 was involved in the upregulation of interleukin (IL)-8. In human samples, HMGB1 was translocated from nucleus to the cytoplasm in hypoxic-conditioned nasal mucosa. HMGB1 was increased in nasal lavage samples of chronic rhinosinusitis patients, whose sinus mucosa was supposed to be hypoxic as compared with controls. We suggest that HMGB1 is secreted in hypoxic condition via ROS-dependent mechanism and secreted HMGB1 participates in IL-8 upregulation mediating inflammatory response.

Original languageEnglish
Pages (from-to)685-694
Number of pages10
JournalMucosal Immunology
Volume10
Issue number3
DOIs
Publication statusPublished - 2017 May 1

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Nasal Mucosa
Interleukin-8
Reactive Oxygen Species
Up-Regulation
Epithelial Cells
Enzyme-Linked Immunosorbent Assay
Nasal Lavage Fluid
Nasal Lavage
Therapeutic Irrigation
Fluorescent Antibody Technique
Oxidoreductases
Cytoplasm
Mucous Membrane
Western Blotting
Immunohistochemistry

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Min, H. J. ; Kim, J. H. ; Yoo, J. E. ; Oh, J. H. ; Kim, K. S. ; Yoon, J. H. ; Kim, Chang-Hoon. / ROS-dependent HMGB1 secretion upregulates IL-8 in upper airway epithelial cells under hypoxic condition. In: Mucosal Immunology. 2017 ; Vol. 10, No. 3. pp. 685-694.
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ROS-dependent HMGB1 secretion upregulates IL-8 in upper airway epithelial cells under hypoxic condition. / Min, H. J.; Kim, J. H.; Yoo, J. E.; Oh, J. H.; Kim, K. S.; Yoon, J. H.; Kim, Chang-Hoon.

In: Mucosal Immunology, Vol. 10, No. 3, 01.05.2017, p. 685-694.

Research output: Contribution to journalArticle

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