S1 domain-containing STF modulates plastid transcription and chloroplast biogenesis in Nicotiana benthamiana

Young Jeon, Hyun Ju Jung, Hunseung Kang, Youn Il Park, Soon Hee Lee, Hyun Sook Pai

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

• In this study, we examined the biochemical and physiological functions of Nicotiana benthamiana S1 domain-containing Transcription-Stimulating Factor (STF) using virus-induced gene silencing (VIGS), cosuppression, and overexpression strategies. • STF:green fluorescent protein (GFP) fusion protein colocalized with sulfite reductase (SiR), a chloroplast nucleoid-associated protein also present in the stroma. Full-length STF and its S1 domain preferentially bound to RNA, probably in a sequence-nonspecific manner. • STF silencing by VIGS or cosuppression resulted in severe leaf yellowing caused by disrupted chloroplast development. STF deficiency significantly perturbed plastid-encoded multimeric RNA polymerase (PEP)-dependent transcript accumulation. Chloroplast transcription run-on assays revealed that the transcription rate of PEP-dependent plastid genes was reduced in the STF-silenced leaves. Conversely, the exogenously added recombinant STF protein increased the transcription rate, suggesting a direct role of STF in plastid transcription. Etiolated seedlings of STF cosuppression lines showed defects in the light-triggered transition from etioplasts to chloroplasts, accompanied by reduced light-induced expression of plastid-encoded genes. • These results suggest that STF plays a critical role as an auxiliary factor of the PEP transcription complex in the regulation of plastid transcription and chloroplast biogenesis in higher plants.

Original languageEnglish
Pages (from-to)349-363
Number of pages15
JournalNew Phytologist
Volume193
Issue number2
DOIs
Publication statusPublished - 2012 Jan 1

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Plastids
Nicotiana benthamiana
Chloroplasts
plastids
Tobacco
chloroplasts
transcription (genetics)
RNA Interference
Gene Silencing
gene silencing
Oxidoreductases Acting on Sulfur Group Donors
Transcription Factors
Viruses
etioplasts
Light
viruses
gene overexpression
Proteins
proteins
sulfites

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

Cite this

Jeon, Young ; Jung, Hyun Ju ; Kang, Hunseung ; Park, Youn Il ; Lee, Soon Hee ; Pai, Hyun Sook. / S1 domain-containing STF modulates plastid transcription and chloroplast biogenesis in Nicotiana benthamiana. In: New Phytologist. 2012 ; Vol. 193, No. 2. pp. 349-363.
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S1 domain-containing STF modulates plastid transcription and chloroplast biogenesis in Nicotiana benthamiana. / Jeon, Young; Jung, Hyun Ju; Kang, Hunseung; Park, Youn Il; Lee, Soon Hee; Pai, Hyun Sook.

In: New Phytologist, Vol. 193, No. 2, 01.01.2012, p. 349-363.

Research output: Contribution to journalArticle

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