S6 kinase 1 plays a key role in mitochondrial morphology and cellular energy flow

Quangdon Tran, Jae Hun Jung, Jisoo Park, Hyunji Lee, Youngeun Hong, Hyeonjeong Cho, Minhee Kim, Sungjin Park, So Hee Kwon, Seon Hwan Kim, George Thomas, Kwang Pyo Kim, Myung Haing Cho, Jongsun Park

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Mitochondrial morphology, which is associated with changes in metabolism, cell cycle, cell development and cell death, is tightly regulated by the balance between fusion and fission. In this study, we found that S6 kinase 1 (S6K1) contributes to mitochondrial dynamics, homeostasis and function. Mouse embryo fibroblasts lacking S6K1 (S6K1-KO MEFs) exhibited more fragmented mitochondria and a higher level of Dynamin related protein 1 (Drp1) and active Drp1 (pS616) in both whole cell extracts and mitochondrial fraction. In addition, there was no evidence for autophagy and mitophagy induction in S6K1 depleted cells. Glycolysis and mitochondrial respiratory activity was higher in S6K1-KO MEFs, whereas OxPhos ATP production was not altered. However, inhibition of Drp1 by Mdivi1 (Drp1 inhibitor) resulted in higher OxPhos ATP production and lower mitochondrial membrane potential. Taken together the depletion of S6K1 increased Drp1-mediated fission, leading to the enhancement of glycolysis. The fission form of mitochondria resulted in lower yield for OxPhos ATP production as well as in higher mitochondrial membrane potential. Thus, these results have suggested a potential role of S6K1 in energy metabolism by modulating mitochondrial respiratory capacity and mitochondrial morphology.

Original languageEnglish
Pages (from-to)13-24
Number of pages12
JournalCellular Signalling
Volume48
DOIs
Publication statusPublished - 2018 Aug

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Ribosomal Protein S6 Kinases
Dynamin I
Dynamins
Adenosine Triphosphate
Mitochondrial Membrane Potential
Glycolysis
Proteins
Mitochondria
Mitochondrial Degradation
Mitochondrial Dynamics
Autophagy
Cell Extracts
Energy Metabolism
Cell Cycle
Homeostasis
Cell Death
Embryonic Structures
Fibroblasts

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Tran, Q., Jung, J. H., Park, J., Lee, H., Hong, Y., Cho, H., ... Park, J. (2018). S6 kinase 1 plays a key role in mitochondrial morphology and cellular energy flow. Cellular Signalling, 48, 13-24. https://doi.org/10.1016/j.cellsig.2018.04.002
Tran, Quangdon ; Jung, Jae Hun ; Park, Jisoo ; Lee, Hyunji ; Hong, Youngeun ; Cho, Hyeonjeong ; Kim, Minhee ; Park, Sungjin ; Kwon, So Hee ; Kim, Seon Hwan ; Thomas, George ; Kim, Kwang Pyo ; Cho, Myung Haing ; Park, Jongsun. / S6 kinase 1 plays a key role in mitochondrial morphology and cellular energy flow. In: Cellular Signalling. 2018 ; Vol. 48. pp. 13-24.
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abstract = "Mitochondrial morphology, which is associated with changes in metabolism, cell cycle, cell development and cell death, is tightly regulated by the balance between fusion and fission. In this study, we found that S6 kinase 1 (S6K1) contributes to mitochondrial dynamics, homeostasis and function. Mouse embryo fibroblasts lacking S6K1 (S6K1-KO MEFs) exhibited more fragmented mitochondria and a higher level of Dynamin related protein 1 (Drp1) and active Drp1 (pS616) in both whole cell extracts and mitochondrial fraction. In addition, there was no evidence for autophagy and mitophagy induction in S6K1 depleted cells. Glycolysis and mitochondrial respiratory activity was higher in S6K1-KO MEFs, whereas OxPhos ATP production was not altered. However, inhibition of Drp1 by Mdivi1 (Drp1 inhibitor) resulted in higher OxPhos ATP production and lower mitochondrial membrane potential. Taken together the depletion of S6K1 increased Drp1-mediated fission, leading to the enhancement of glycolysis. The fission form of mitochondria resulted in lower yield for OxPhos ATP production as well as in higher mitochondrial membrane potential. Thus, these results have suggested a potential role of S6K1 in energy metabolism by modulating mitochondrial respiratory capacity and mitochondrial morphology.",
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Tran, Q, Jung, JH, Park, J, Lee, H, Hong, Y, Cho, H, Kim, M, Park, S, Kwon, SH, Kim, SH, Thomas, G, Kim, KP, Cho, MH & Park, J 2018, 'S6 kinase 1 plays a key role in mitochondrial morphology and cellular energy flow', Cellular Signalling, vol. 48, pp. 13-24. https://doi.org/10.1016/j.cellsig.2018.04.002

S6 kinase 1 plays a key role in mitochondrial morphology and cellular energy flow. / Tran, Quangdon; Jung, Jae Hun; Park, Jisoo; Lee, Hyunji; Hong, Youngeun; Cho, Hyeonjeong; Kim, Minhee; Park, Sungjin; Kwon, So Hee; Kim, Seon Hwan; Thomas, George; Kim, Kwang Pyo; Cho, Myung Haing; Park, Jongsun.

In: Cellular Signalling, Vol. 48, 08.2018, p. 13-24.

Research output: Contribution to journalArticle

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AU - Tran, Quangdon

AU - Jung, Jae Hun

AU - Park, Jisoo

AU - Lee, Hyunji

AU - Hong, Youngeun

AU - Cho, Hyeonjeong

AU - Kim, Minhee

AU - Park, Sungjin

AU - Kwon, So Hee

AU - Kim, Seon Hwan

AU - Thomas, George

AU - Kim, Kwang Pyo

AU - Cho, Myung Haing

AU - Park, Jongsun

PY - 2018/8

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