S6K1 controls epigenetic plasticity for the expression of pancreatic α/β cell marker genes

Sang Ah Yi, Jieun Lee, Jong Woo Park, Jihoon Han, Min Gyu Lee, Ki Hong Nam, Jee Hun Park, Hwamok Oh, Sung Jin Ahn, Saetbyul Kim, So Hee Kwon, Dong Gyu Jo, Jeung Whan Han

Research output: Contribution to journalArticle

Abstract

The failure of insulin production by pancreatic β cells is a common hallmark of type 1 diabetes mellitus (T1DM). Because administration of exogenous insulin is associated with diabetes-derived complications, endogenous α to β cell transition can be an attractive alternative. Although decreased β cell size and hypoinsulinaemia have been observed in S6K1-deficient mice, the molecular mechanism underlying the involvement of S6K1 in the transcriptional regulation of insulin remains elusive. Here, we show that the hypoinsulinaemic phenotype of S6K1-deficient mice stems from the dysregulated transcription of a set of genes required for insulin and glucagon production. First, we observed that increased expression of α cell marker genes and decreased expression of β cell marker genes in pancreas tissues from S6K1-deficient mice. Furthermore, S6K1 was highly activated in murine β cell line, βTC6, compared to murine α cell line αTC1. In both α and β cells, active S6K1 promoted the transcription of β cell marker genes, including insulin, whereas S6K1 inhibition increased the transcription of α cell marker genes. Moreover, S6K1 mediated pancreatic gene regulation by modifying two histone marks (activating H3K4me3 and repressing H3K27me3) on gene promoters. These results suggest that S6K1 drives the α to β transition through the epigenetic regulation of cell-specific genes, including insulin and glucagon. This novel role of S6K1 in islet cells provides basic clues to establish therapeutic strategies against T1DM.

Original languageEnglish
Pages (from-to)6674-6683
Number of pages10
JournalJournal of Cellular Biochemistry
Volume119
Issue number8
DOIs
Publication statusPublished - 2018 Aug

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Epigenomics
Plasticity
Genes
Insulin
Cells
Transcription
Medical problems
Glucagon
Type 1 Diabetes Mellitus
Histone Code
Cell Line
Gene expression
Histones
Diabetes Complications
Islets of Langerhans
Cell Size
Pancreas
Tissue
Phenotype
Gene Expression

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Yi, S. A., Lee, J., Park, J. W., Han, J., Lee, M. G., Nam, K. H., ... Han, J. W. (2018). S6K1 controls epigenetic plasticity for the expression of pancreatic α/β cell marker genes. Journal of Cellular Biochemistry, 119(8), 6674-6683. https://doi.org/10.1002/jcb.26853
Yi, Sang Ah ; Lee, Jieun ; Park, Jong Woo ; Han, Jihoon ; Lee, Min Gyu ; Nam, Ki Hong ; Park, Jee Hun ; Oh, Hwamok ; Ahn, Sung Jin ; Kim, Saetbyul ; Kwon, So Hee ; Jo, Dong Gyu ; Han, Jeung Whan. / S6K1 controls epigenetic plasticity for the expression of pancreatic α/β cell marker genes. In: Journal of Cellular Biochemistry. 2018 ; Vol. 119, No. 8. pp. 6674-6683.
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Yi, SA, Lee, J, Park, JW, Han, J, Lee, MG, Nam, KH, Park, JH, Oh, H, Ahn, SJ, Kim, S, Kwon, SH, Jo, DG & Han, JW 2018, 'S6K1 controls epigenetic plasticity for the expression of pancreatic α/β cell marker genes', Journal of Cellular Biochemistry, vol. 119, no. 8, pp. 6674-6683. https://doi.org/10.1002/jcb.26853

S6K1 controls epigenetic plasticity for the expression of pancreatic α/β cell marker genes. / Yi, Sang Ah; Lee, Jieun; Park, Jong Woo; Han, Jihoon; Lee, Min Gyu; Nam, Ki Hong; Park, Jee Hun; Oh, Hwamok; Ahn, Sung Jin; Kim, Saetbyul; Kwon, So Hee; Jo, Dong Gyu; Han, Jeung Whan.

In: Journal of Cellular Biochemistry, Vol. 119, No. 8, 08.2018, p. 6674-6683.

Research output: Contribution to journalArticle

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T1 - S6K1 controls epigenetic plasticity for the expression of pancreatic α/β cell marker genes

AU - Yi, Sang Ah

AU - Lee, Jieun

AU - Park, Jong Woo

AU - Han, Jihoon

AU - Lee, Min Gyu

AU - Nam, Ki Hong

AU - Park, Jee Hun

AU - Oh, Hwamok

AU - Ahn, Sung Jin

AU - Kim, Saetbyul

AU - Kwon, So Hee

AU - Jo, Dong Gyu

AU - Han, Jeung Whan

PY - 2018/8

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N2 - The failure of insulin production by pancreatic β cells is a common hallmark of type 1 diabetes mellitus (T1DM). Because administration of exogenous insulin is associated with diabetes-derived complications, endogenous α to β cell transition can be an attractive alternative. Although decreased β cell size and hypoinsulinaemia have been observed in S6K1-deficient mice, the molecular mechanism underlying the involvement of S6K1 in the transcriptional regulation of insulin remains elusive. Here, we show that the hypoinsulinaemic phenotype of S6K1-deficient mice stems from the dysregulated transcription of a set of genes required for insulin and glucagon production. First, we observed that increased expression of α cell marker genes and decreased expression of β cell marker genes in pancreas tissues from S6K1-deficient mice. Furthermore, S6K1 was highly activated in murine β cell line, βTC6, compared to murine α cell line αTC1. In both α and β cells, active S6K1 promoted the transcription of β cell marker genes, including insulin, whereas S6K1 inhibition increased the transcription of α cell marker genes. Moreover, S6K1 mediated pancreatic gene regulation by modifying two histone marks (activating H3K4me3 and repressing H3K27me3) on gene promoters. These results suggest that S6K1 drives the α to β transition through the epigenetic regulation of cell-specific genes, including insulin and glucagon. This novel role of S6K1 in islet cells provides basic clues to establish therapeutic strategies against T1DM.

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